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CD362/SDC2/Syndecan-2 Antibody (F-5) - Western Blotting - Image 154113 — Syndecan-2 抗体 (F-5): sc-376160. 非转染: sc-117752 (A) 和小鼠 Syndecan-2 转染: sc-123870 (B) 293T 全细胞裂解液中 Syndecan-2 表达的 Western 印迹分析.

Syndecan-2 抗体 (F-5): sc-376160. 非转染: sc-117752 (A) 和小鼠 Syndecan-2 转染: sc-123870 (B) 293T 全细胞裂解液中 Syndecan-2 表达的 Western 印迹分析.

CD362/SDC2/Syndecan-2 抗体 (F-5): sc-376160

Name: CD362/SDC2/Syndecan-2抗体 (F-5)
Brand: SCBIO
SKU: sc-376160
Price: 2377.0 CNY
Availability: InStock

5.0(4)

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说明书

CD362/SDC2/Syndecan-2抗体(F-5)是小鼠单克隆IgG₁ κ, 在3篇文献中引用，规格为200 µg/ml
特定于mouse起源的Syndecan-2的N-terminal胞外结构域内氨基酸59-95之间的表位图谱
抗-CD362/SDC2/Syndecan-2 抗体 (F-5) 推荐用于 WB, IP, IF 和 ELISA，检测mouse 和rat 来源的 Syndecan-2
抗CD362/SDC2/Syndecan-2抗体（F-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取CD362/SDC2/Syndecan-2 (F-5): sc-376160的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
1 BP-HRP">m-IgG₁ BP-HRP和m-IgGκ BP-HRP是CD362/SDC2/Syndecan-2 Antibody (F-5) 适用于 WB 应用。的首选辅助检测试剂。这些试剂现与CD362/SDC2/Syndecan-2 Antibody (F-5) 打包提供（请参阅下面的订购信息）。

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関連項目

额外Syndecan抗体，包括Syndecan-1, Syndecan-2, Syndecan-3和Syndecan-4

CD362/SDC2/Syndecan-2抗体（F-5）是一种IgG1 κ小鼠单克隆Syndecan-2抗体，可通过WB、IP、IF和ELISA检测小鼠和大鼠来源的Syndecan-2。CD362/SDC2/Syndecan-2抗体（F-5）可作为非偶联抗Syndecan-2抗体形式提供，也可作为多种偶联形式的抗Syndecan-2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Syndecans是I型整体膜蛋白聚糖，含有硫酸软骨素和硫酸乙酰肝素基团。Syndecans参与细胞-细胞外基质粘附和生长因子结合。Syndecan-1（SYND1，也称为CD138）是一种细胞外基质受体，与胶原蛋白、纤维连接蛋白和血小板反应蛋白结合。Syndecan-1和Syndecan-3（也称为N-Syndecan）与MK（中期因子）相互作用，MK是一种参与中枢神经系统胚胎发育的生长/分化因子。Syndecan-2（也称为fibroglycan或HSPG）在形态发生活性高的区域，如上皮-间充质界面以及前软骨和前成骨间充质凝集中高度表达。Syndecan-4（也称为amphiglycan或ryudocan）在细胞扩散、局灶性粘附组装和肌动蛋白应力纤维组装过程中与整合素协同作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor^® 是Molecular Probes Inc., OR., USA的商标

LI-COR^®和 Odyssey^® 是LI-COR Biosciences的注册商标

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
CD362/SDC2/Syndecan-2 抗体 (F-5)	sc-376160	200 µg/ml	RMB2377.00
CD362/SDC2/Syndecan-2 (F-5): m-IgGκ BP-HRP 套装	sc-522856	200 µg Ab, 40 µg BP	RMB2662.00
CD362/SDC2/Syndecan-2 (F-5): m-IgG₁ BP-HRP 套装	sc-533528	200 µg Ab; 20 µg BP	RMB2662.00
CD362/SDC2/Syndecan-2 抗体 (F-5) AC	sc-376160 AC	500 µg/ml, 25% agarose	RMB3129.00
CD362/SDC2/Syndecan-2 抗体 (F-5) HRP	sc-376160 HRP	200 µg/ml	RMB2377.00
CD362/SDC2/Syndecan-2 抗体 (F-5) FITC	sc-376160 FITC	200 µg/ml	RMB2482.00
CD362/SDC2/Syndecan-2 抗体 (F-5) PE	sc-376160 PE	200 µg/ml	RMB2580.00
CD362/SDC2/Syndecan-2 抗体 (F-5) Alexa Fluor^® 488	sc-376160 AF488	200 µg/ml	RMB2685.00
CD362/SDC2/Syndecan-2 抗体 (F-5) Alexa Fluor^® 546	sc-376160 AF546	200 µg/ml	RMB2685.00
CD362/SDC2/Syndecan-2 抗体 (F-5) Alexa Fluor^® 594	sc-376160 AF594	200 µg/ml	RMB2685.00
CD362/SDC2/Syndecan-2 抗体 (F-5) Alexa Fluor^® 647	sc-376160 AF647	200 µg/ml	RMB2685.00
CD362/SDC2/Syndecan-2 抗体 (F-5) Alexa Fluor^® 680	sc-376160 AF680	200 µg/ml	RMB2685.00
CD362/SDC2/Syndecan-2 抗体 (F-5) Alexa Fluor^® 790	sc-376160 AF790	200 µg/ml	RMB2685.00
CD362/SDC2/Syndecan-2 (F-5) 中和勝肽	sc-376160 P	100 µg/0.5 ml	RMB511.00

The datasheet for this antibody says that it is specific for an epitope mapping between amino acids 59-95 within an internal region of ms Syndecan-2. However, amino acids 19–145 in ms Syndecan-2 are the extracellular, not cytoplasmic (internal?) domain?

Asked by: TFL Wishart

Thank you for your question. This antibody does have an epitope that is mapping within the extracellular region of Syndecan-2. The description of the epitope mapping within an "internal region" is misleading, and will be updated; it was intended to describe the epitope mapping as neither N-terminal, or C-terminal, but rather in between.

Answered by: Technical Support

Date published: 2019-07-10

Can you please clarify this statement about the antibody being "specific for an epitope mapping between amino acids 59-95 within an internal region of Syndecan-2 of mouse origin"? AA 59-95 are within the ectodomain of syndecan-2, so how is this possible?

Asked by: Tincan615

Thank you for your question. The term 'internal region' refers to the epitope being neither N-terminal nor C-terminal. It does not specify whether the epitope is within an intracellular or extracellular domain of the protein.

Answered by: Tech Support Europe

Date published: 2019-05-28

The description states that sc-376160 is "specific for an epitope mapping between amino acids 59-95 within an internal region of Syndecan-2 of mouse origin". Amino acids 59-95 reside in the extracellular portion of Syndecan-2. Can you please explain?

Asked by: ctermini

Thank you for your question. The epitope for this antibody maps within amino acids 59-95 of Syndecan-2 of mouse origin (accession#P43407). The exact epitope has not been mapped.

Answered by: Technical Support

Date published: 2019-01-26

What application is the blocking peptide sc-376160 P appropriate for?

Asked by: AbPolly

Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization

Answered by: Technical Support

Date published: 2017-02-28

Rated 5 out of 5 by caijin from work wellThis antibody work well in the western blot with the dilution 1:1000.

Date published: 2017-06-03

Rated 5 out of 5 by Junyi from Good ihc data.We usually use the dilution 1:800, bands came out very clear.Good ihc data.

Date published: 2017-04-28

Rated 5 out of 5 by herobar from good working qualityClean band in western blot analysis of MCT2 expression in mouse heart tissue extract,a good antibody.

Date published: 2017-02-12

Rated 5 out of 5 by TO from Produced positive Western blot data of SyndecanProduced positive Western blot data of Syndecan-2 expression in non-transfected and mouse Syndecan-2 transfected 293T whole cell lysates. -SCBT QC

Date published: 2013-12-07

CD362/SDC2/Syndecan-2 抗体 (F-5) is rated 5.0 out of 5 by 4.

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CD362/SDC2/Syndecan-2 抗体 (F-5): sc-376160

CD362/SDC2/Syndecan-2 抗体 (F-5) 规格

替代产品名称: Syndecan-2

产品克隆名称: F-5

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: 59-95

抗原表位靶标: Syndecan-2

抗原表位种属: mouse

产品反应性/检测: Syndecan-2

种属反应性/检测: mouse, rat

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 3篇各种科学出版物的引文

優選的 CD362/SDC2/Syndecan-2 抗體是 CD362/SDC2/Syndecan-2 抗體 (H-7): sc-365624.