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SV40 T Ag Antibody (PAb419) - Western Blotting - Image 15630
SV40 T Ag (Pab 419): sc-58665. GM637 (A) 和 XP12RO (B) 全细胞裂解液中 SV40 T Ag 表达的 Western 印迹分析.
SV40 T Ag Antibody (PAb419) - Western Blotting - Image 15630
SV40 T Ag (Pab 419): sc-58665. GM637 (A) 和 XP12RO (B) 全细胞裂解液中 SV40 T Ag 表达的 Western 印迹分析.
SV40 T Ag (Pab 419): sc-58665. GM637 (A) 和 XP12RO (B) 全细胞裂解液中 SV40 T Ag 表达的 Western 印迹分析.

SV40 T Ag 抗体 (PAb419): sc-58665

3.7(3)
寫評論提問

说明书
  • SV40 T Ag抗体(PAb419)是小鼠单克隆IgG2a κ, 在13篇文献中引用，规格为200 µg/ml
  • 针对全长T抗原的抗体，源自SV40
  • 推荐用于 SV40 来源的N-terminal domain of large and small T antigen WB, IP, IF 和 ELISA检测
  • 看 SV40 T Ag (Pab 101): sc-147，可查看有标记的 SV40 T Ag 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是SV40 T Ag Antibody (PAb419) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与SV40 T Ag Antibody (PAb419) 打包提供（请参阅下面的订购信息）。

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猿猴病毒SV40为研究恶性转化中的细胞机制提供了重要模型。主要的SV40翻译产物包括大T抗原和小T抗原，两者均由SV40病毒基因组的早期区域编码。大T抗原与p53抑制基因复合，导致其功能失活，从而促进细胞转化。此外，SV40大T抗原与DNA聚合酶和转录因子AP-2结合。它还与第二个肿瘤抑制基因编码的蛋白质Rb 105形成复合物。SV40 T抗原的结合对Rb p105的"pocket"结构域具有特异性，该结构域也是E2F细胞转录因子的结合位点。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

SV40 T Ag 抗体 (PAb419)

sc-58665
200 µg/ml
RMB2377.00

SV40 T Ag (PAb419): m-IgG Fc BP-HRP 套装

sc-539091
200 µg Ab; 10 µg BP
RMB2662.00

SV40 T Ag (PAb419): m-IgG2a BP-HRP 套装

sc-546668
200 µg Ab; 10 µg BP
RMB2662.00

What is the recommended secondary for SV40 T Ag (Pab 419): sc-58665 monoclonal antibody?

Asked by: cjMara
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-03-07
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Rated 1 out of 5 by from It did not work at all. Western Blot.I tried a dilution of even 1:100 and had no signal whatsoever. I made sure -of course- that the secondary-HRP work (it does) and that the proteins contained the proteins to be recognized (by using another monoclonal, which it worked).
Date published: 2020-12-01
Rated 5 out of 5 by from WB data published with tissue culture cellsWB data published with tissue culture cells - SCBT Publication Review
Date published: 2015-02-15
Rated 5 out of 5 by from Antibody published in WB with mice IDGAntibody published in WB with mice IDG-SW3 cells - SCBT Publication Review
Date published: 2014-01-29
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SV40 T Ag 抗体 (PAb419) is rated 3.7 out of 5 by 3.
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