Date published: 2025-9-11
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SV40 T Ag Antibody (Pab 108) - Western Blotting - Image 412949
SV40 T Ag 抗体（Pab 108）：sc-148。近红外蛋白质印迹分析 MLZ（A）和 GM637（B）全细胞裂解物中 SV40 T Ag 的表达。用 UltraCruz® 阻断试剂阻断：sc-516214。检测试剂：m-IgG2a BP-CFL 680：sc-542739。
SV40 T Ag 抗体（Pab 108）：sc-148。近红外蛋白质印迹分析 MLZ（A）和 GM637（B）全细胞裂解物中 SV40 T Ag 的表达。用 UltraCruz® 阻断试剂阻断：sc-516214。检测试剂：m-IgG2a BP-CFL 680：sc-542739。

SV40 T Ag 抗体 (Pab 108): sc-148

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寫評論提問

说明书
  • SV40 T Ag抗体(Pab 108)是小鼠单克隆IgG2a κ, 在61篇文献中引用，规格为200 µg/ml
  • 针对小鼠来源的SV-40转化细胞系B4
  • SV40 T Ag 抗体 (Pab 108) 推荐用于WB, IP, IF 和 IHC(P)，检测SV40 来源的 the N-terminus of large SV40 T antigen, small SV40 T antigen and BK virus T antigen
  • 抗SV40 T Ag抗体（Pab 108）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SV40 T Ag (Pab 108): sc-148的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 SV40 T Ag 抗体 (Pab 108) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SV40 T Ag 抗体 (Pab 108) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
说明书与实验方案
研究信息

関連項目

SV40 T Ag 抗体（Pab 108）是一种鼠单克隆 IgG2a kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测 SV40 起源的 SV40 T Ag。抗SV40 T抗原抗体（Pab 108）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。SV40 T抗原在病毒生命周期中起着至关重要的作用，对于宿主细胞的转化也必不可少，因为SV40 T抗原与调节细胞生长和分裂的关键细胞蛋白相互作用。值得注意的是，SV40大T抗原与视网膜母细胞瘤（Rb）肿瘤抑制蛋白结合，特别针对其"口袋"结构域，这也是E2F转录因子的结合位点。这种相互作用导致Rb失活，从而释放E2F并促进细胞周期进展所需的基因转录。SV40 T抗原能够破坏正常的细胞周期调节，这凸显了它在肿瘤发生和细胞转化研究中的重要性，使SV40 T抗原单克隆抗体（Pab 108）成为研究人员研究癌症发展和病毒发病机制的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

SV40 T Ag 抗体 (Pab 108)

sc-148
200 µg/ml
RMB2377.00

SV40 T Ag (Pab 108): m-IgG Fc BP-HRP 套装

sc-525420
200 µg Ab; 10 µg BP
RMB2662.00

SV40 T Ag (Pab 108): m-IgG2a BP-HRP 套装

sc-546244
200 µg Ab; 10 µg BP
RMB2662.00

SV40 T Ag 抗体 (Pab 108) AC

sc-148 AC
500 µg/ml, 25% agarose
RMB3129.00

SV40 T Ag 抗体 (Pab 108) HRP

sc-148 HRP
200 µg/ml
RMB2377.00

SV40 T Ag 抗体 (Pab 108) FITC

sc-148 FITC
200 µg/ml
RMB2482.00

SV40 T Ag 抗体 (Pab 108) PE

sc-148 PE
200 µg/ml
RMB2580.00

SV40 T Ag 抗体 (Pab 108) Alexa Fluor® 488

sc-148 AF488
200 µg/ml
RMB2685.00

SV40 T Ag 抗体 (Pab 108) Alexa Fluor® 546

sc-148 AF546
200 µg/ml
RMB2685.00

SV40 T Ag 抗体 (Pab 108) Alexa Fluor® 594

sc-148 AF594
200 µg/ml
RMB2685.00

SV40 T Ag 抗体 (Pab 108) Alexa Fluor® 647

sc-148 AF647
200 µg/ml
RMB2685.00

SV40 T Ag 抗体 (Pab 108) Alexa Fluor® 680

sc-148 AF680
200 µg/ml
RMB2685.00

SV40 T Ag 抗体 (Pab 108) Alexa Fluor® 790

sc-148 AF790
200 µg/ml
RMB2685.00

I am using SV40 T Ag (Pab 108): sc-148 to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
Yes, this antibody has been validated for use in IHC with paraffin-embedded tissue sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 7
Date published: 2017-01-24
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Rated 5 out of 5 by from LT-Ag - IF & WBLT-Ag antibody worked well on both Methanol:Acetone (50:50) and 10% formalin fixed slides at 1:200. Western blotting was effective at 1:250.
Date published: 2022-10-04
Rated 3 out of 5 by from Good antibody for immunofluorescenceGood antibody for immunofluorescence of SV40LT-transduced cells but unsuitable for WB. I've tested it for WB in several human SV40LT-transduced cell lines (including TC28a2 chondrocytes and immortalized MSC lines) and several non-specific bands were present for all transduced and non-transduced cells.
Date published: 2021-03-25
Rated 4 out of 5 by from good productIt works ok for our western blot experiment in our lab.
Date published: 2018-08-22
Rated 2 out of 5 by from Non specific bandsI did a western blot in order to detect the expression of SV40 LT Ag in SV40 LT transfected human cells. I've tried blocking with milk 5% and BSA 3% several times but I am always getting the same result: one specific band of approximately 90 kDa, but accompained by non specific bands (the most intense very near to the specific band of 90 kDa, apppearing in both negative and positive controls). I've tried primary mesenchymal stem cells (MSCs) from several patients as negative controls and T/C28a2 cell line anlong with Phoenix-A cell line (derived from 293T cell line) -both known to express SV40 LT Ag- as positive controls.
Date published: 2017-02-06
Rated 5 out of 5 by from Specific for SV40 T AgConsistency is important in research! This antibody fits the bill, nice and reliable, strong bands in WB and specific staining in IHC.
Date published: 2016-12-30
Rated 5 out of 5 by from Robust signal in WB in MLZRobust signal in WB in MLZ, GM637 and RAW 264.7 whole cell lysates. -SCBT QC
Date published: 2015-05-03
Rated 5 out of 5 by from Published in IHC in mouse brain tissuePublished in IHC in mouse brain tissue. -SCBT Publication Review
Date published: 2015-05-01
Rated 5 out of 5 by from Published for IPPublished for IP, WB and IF in human WI38 and BJ. -SCBT Publication Review
Date published: 2015-04-14
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SV40 T Ag 抗体 (Pab 108) is rated 4.5 out of 5 by 11.
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