Date published: 2025-9-9

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Sulfiredoxin 抗体 (E-2): sc-166747

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说明书
  • Sulfiredoxin 抗体 E-2 是小鼠单克隆 IgG2b κ,Sulfiredoxin抗体, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Sulfiredoxin的C-terminus的氨基酸121-137之间
  • Sulfiredoxin 抗体 (E-2) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Sulfiredoxin; 也和以下物种反应,包括: 和 equine, bovine and porcine
  • 抗Sulfiredoxin抗体(E-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Sulfiredoxin (E-2): sc-166747免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是Sulfiredoxin Antibody (E-2) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Sulfiredoxin Antibody (E-2) 打包提供(请参阅下面的订购信息)。

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関連項目

Sulfiredoxin抗体(E-2)是一种IgG2b κ鼠单克隆Sulfiredoxin抗体,可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源性的Sulfiredoxin蛋白。Sulfiredoxin抗体(E-2)既可以是非偶联的抗Sulfiredoxin抗体形式,也可以是多种偶联形式的抗Sulfiredoxin抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Sulfiredoxin,也称为Sulfiredoxin-1和染色体20开放阅读框139(C20orf139),是一种细胞质抗氧化蛋白,通过催化减少氧化修饰参与信号传导。它通过将保守的半胱氨酸从亚磺酸还原为亚磺酸来调节过氧化物酶(PRXs),这是一类减少过氧化氢的蛋白质家族。这影响了PRX在其他下游转录因子和激酶信号通路中的作用。Sulfiredoxin蛋白特异性地作用于PRDX1、PRDX2、PRDX3和PRDX4过氧化物酶,但不作用于PRDX5或PRDX6。Sulfiredoxin作为磷酸转移酶和硫醇转移酶起作用,并且在体内广泛表达,在肺、脾、肾和胸腺组织中检测到最高水平。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Sulfiredoxin 抗体 (E-2) 参考文献:

  1. 哺乳动物磺胺氧化还原酶的特征及其通过将活性位点的半胱氨酸亚硫酸还原为半胱氨酸而重新激活过氧化过氧化还原酶。  |  Chang, TS., et al. 2004. J Biol Chem. 279: 50994-1001. PMID: 15448164
  2. 磺胺氧化还原半胱氨酸亚硫酸是 2-cys过氧化还原酶的特异性作用。  |  Woo, HA., et al. 2005. J Biol Chem. 280: 3125-8. PMID: 15590625
  3. 真核生物 2-Cys 过氧化还原酶的氧化作用是一个分子开关,它控制着对不断增加的过氧化氢水平的转录反应。  |  Bozonet, SM., et al. 2005. J Biol Chem. 280: 23319-27. PMID: 15824112
  4. 真核半胱氨酸亚硫酸还原酶 sulfiredoxin (Srx) 从细菌染色体分离蛋白 ParB 演化而来。  |  Basu, MK. and Koonin, EV. 2005. Cell Cycle. 4: 947-52. PMID: 15917647
  5. 人硫脂还原酶逆还原失活过氧化物酶的结构基础。  |  Jönsson, TJ., et al. 2005. Biochemistry. 44: 8634-42. PMID: 15952770
  6. 磺胺毒素:一种潜在的治疗剂?  |  Findlay, VJ., et al. 2005. Biomed Pharmacother. 59: 374-9. PMID: 16102934
  7. 人类磺胺毒素(hSrx)的 1H, 15N 和 13C 化学位移分布。  |  Lee, DY., et al. 2005. J Biomol NMR. 32: 339. PMID: 16211494
  8. 硫氧化还原蛋白,一种2-半胱氨酸过氧化还原蛋白特异的半胱氨酸亚磺酸还原酶:其发现、作用机制和生物学意义。  |  Rhee, SG., et al. 2007. Kidney Int Suppl. S3-8. PMID: 17653208
  9. 硫氧还蛋白还原真核生物中典型的2-半胱氨酸过氧化还原酶中的半胱氨酸亚磺酸。  |  Lowther, WT. and Haynes, AC. 2011. Antioxid Redox Signal. 15: 99-109. PMID: 20712415
  10. 硫氧化还原蛋白/过氧化还原蛋白/硫氧化还原蛋白系统:目前关于其在植物中的氧化还原功能以及活性氧和氮调节的概述。  |  Sevilla, F., et al. 2015. J Exp Bot. 66: 2945-55. PMID: 25873657
  11. 硫氧还蛋白通过调节miR143-Fascin轴促进癌细胞侵袭。  |  Jiang, H., et al. 2022. Mol Cell Biol. 42: e0005122. PMID: 35412358
  12. 糖尿病患者牙周炎中硫氧还蛋白和总氧化应激水平的比较分析:病例对照研究。  |  Tk, F., et al. 2023. J Periodontol. 94: 785-792. PMID: 36542371

订购信息

产品名称产品编号规格价格数量收藏夹

Sulfiredoxin 抗体 (E-2)

sc-166747
200 µg/ml
RMB2377.00

Sulfiredoxin (E-2): m-IgGκ BP-HRP 套装

sc-521730
200 µg Ab, 40 µg BP
RMB2662.00

Sulfiredoxin (E-2): m-IgG2b BP-HRP 套装

sc-548961
200 µg Ab; 10 µg BP
RMB2662.00

Sulfiredoxin 抗体 (E-2) AC

sc-166747 AC
500 µg/ml, 25% agarose
RMB3129.00

Sulfiredoxin 抗体 (E-2) HRP

sc-166747 HRP
200 µg/ml
RMB2377.00

Sulfiredoxin 抗体 (E-2) FITC

sc-166747 FITC
200 µg/ml
RMB2482.00

Sulfiredoxin 抗体 (E-2) PE

sc-166747 PE
200 µg/ml
RMB2580.00

Sulfiredoxin 抗体 (E-2) Alexa Fluor® 488

sc-166747 AF488
200 µg/ml
RMB2685.00

Sulfiredoxin 抗体 (E-2) Alexa Fluor® 546

sc-166747 AF546
200 µg/ml
RMB2685.00

Sulfiredoxin 抗体 (E-2) Alexa Fluor® 594

sc-166747 AF594
200 µg/ml
RMB2685.00

Sulfiredoxin 抗体 (E-2) Alexa Fluor® 647

sc-166747 AF647
200 µg/ml
RMB2685.00

Sulfiredoxin 抗体 (E-2) Alexa Fluor® 680

sc-166747 AF680
200 µg/ml
RMB2685.00

Sulfiredoxin 抗体 (E-2) Alexa Fluor® 790

sc-166747 AF790
200 µg/ml
RMB2685.00

Sulfiredoxin (E-2) 中和勝肽

sc-166747 P
100 µg/0.5 ml
RMB511.00

Dear sirs, can you provide the information on optimal antibody dillution for flow cytometry? Thank you in advance! Best regards, Katja

Asked by: flow_q
Thank you for your question. We recommend using 1 µg of fluorochrome-conjugated antibodies to tubes containing 1 million cells in 100 µl.
Answered by: Tech Support Europe
Date published: 2025-08-27

What application is the blocking peptide sc-166747 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 1 out of 5 by from No detection in mouse macrophagesWe tried W Blots several times, but in mouse macrophages even with Ab dillution 1:100, we could not detect sulfiredoxin. Perhaps the Ab sample got frozen in the frige? Is it possible that the frozen sample does not work at all, or it just has lower affinity?
Date published: 2018-02-27
Rated 5 out of 5 by from Satisfactory Western Blot data of SulfiredoxinSatisfactory Western Blot data of Sulfiredoxin expression in non-transfected and human Sulfiredoxin transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-02-28
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Sulfiredoxin 抗体 (E-2) is rated 3.0 out of 5 by 2.
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