Date published: 2025-9-8
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Substance P Antibody (NC1/34HL) - Immunohistochemistry - Image 387741
P 物质抗体 (NC1/34HL): sc-21715. 对福尔马林固定, 石蜡包埋的小鼠脑组织进行免疫过氧化物酶染色, 显示神经元细胞和内皮细胞的胞浆染色.
Substance P Antibody (NC1/34HL) - Immunohistochemistry - Image 387741
P 物质抗体 (NC1/34HL): sc-21715. 对福尔马林固定, 石蜡包埋的小鼠脑组织进行免疫过氧化物酶染色, 显示神经元细胞和内皮细胞的胞浆染色.
P 物质抗体 (NC1/34HL): sc-21715. 对福尔马林固定, 石蜡包埋的小鼠脑组织进行免疫过氧化物酶染色, 显示神经元细胞和内皮细胞的胞浆染色.

Substance P 抗体 (NC1/34HL): sc-21715

4.3(6)
寫評論提問

说明书
  • Substance P 抗体 NC1/34HL 是大鼠单克隆 IgG，Substance P抗体, 在27篇文献中引用，规格为200 µg/ml
  • C-terminus 附近的表位图谱
  • Substance P 抗体 (NC1/34HL) 推荐用于IF 和 IHC(P)，检测multiple species 来源的 Substance P and Neurokinins A and B; 不会与 [Leu] enkephalin, [Met] enkephalin, somatostatin and β-endorphin产生交联反应
  • 抗Substance P抗体（NC1/34HL）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Substance P (NC1/34HL): sc-21715的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Substance P 抗体 (NC1/34HL)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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研究信息

関連項目

Tachykinin 抗体

物质P抗体（NC1/34HL）是一种大鼠单克隆IgG，靶向物质P，一种属于激肽家族的神经肽，也称为神经激肽-1（NK1）配体。物质P是一种11个氨基酸的肽，对于传递疼痛信号和调节神经系统的炎症反应至关重要，在疼痛感知、情绪调节和神经源性炎症中发挥着重要作用。物质P大鼠单克隆抗体（NC1/34HL）与物质P C末端附近的表位结合。物质P单克隆抗体（NC1/34HL）与物质P和多种物种来源的神经激肽A和B反应 在免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）应用中，与相关肽（如[Leu]脑啡肽、[Met]脑啡肽、生长抑素或β-内啡肽）无交叉反应。通过检测物质P，抗物质P抗体（NC1/34HL）可以帮助研究人员研究疼痛途径和炎症过程，从而有助于开发治疗神经和免疫疾病的干预措施。物质P抗体（NC1/34HL）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，为不同的实验设置提供了多功能性。了解物质P的功能至关重要，因为物质P与NK1受体相互作用，影响血管舒张、细胞生长和免疫反应，因此物质P成为生物医学研究的重要靶标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Substance P 抗体 (NC1/34HL) 参考文献:

  1. 大脑中的速激肽 NK1 受体：药理学和假定功能。  |  Saria, A. 1999. Eur J Pharmacol. 375: 51-60. PMID: 10443564
  2. P 物质对 B 细胞分化的细胞因子样作用。  |  Pascual, DW., et al. 1992. Reg Immunol. 4: 100-4. PMID: 1380278
  3. P 物质能促进大鼠视前腹外侧区的睡眠。  |  Zhang, G., et al. 2004. Brain Res. 1028: 225-32. PMID: 15527748
  4. 开发自动体外选择协议以获得基于 RNA 的适配体：鉴定生物稳定的 P 物质拮抗剂。  |  Eulberg, D., et al. 2005. Nucleic Acids Res. 33: e45. PMID: 15745995
  5. IL-12 诱导脾脏和炎症部位的小鼠巨噬细胞中编码 P 物质的 mRNA。  |  Arsenescu, R., et al. 2005. J Immunol. 174: 3906-11. PMID: 15778345
  6. 脊髓μ-和δ-阿片激动剂对传入诱发的物质P释放的抑制。  |  Kondo, I., et al. 2005. J Neurosci. 25: 3651-60. PMID: 15814796
  7. P 物质在调节血压和高血压中的作用。  |  Chen, J., et al. 1991. Ann N Y Acad Sci. 632: 413-4. PMID: 1719891
  8. P 物质诱发新生大鼠离体脊髓释放 GABA。  |  Sakuma, M., et al. 1991. Neuroscience. 45: 323-30. PMID: 1722288
  9. 神经肽物质 P 与免疫反应。  |  Mashaghi, A., et al. 2016. Cell Mol Life Sci. 73: 4249-4264. PMID: 27314883
  10. 物质P与纤维化疾病。  |  Peng, L., et al. 2019. Neuropeptides. 76: 101941. PMID: 31256921
  11. 人类 beta-前激肽（物质 P 和神经激肽 A 的共同前体）的 cDNA 序列。  |  Harmar, AJ., et al. 1986. FEBS Lett. 208: 67-72. PMID: 3770210
  12. P 物质诱导小鼠巨噬细胞产生 IL-12  |  Kincy-Cain, T. and Bost, KL. 1997. J Immunol. 158: 2334-9. PMID: 9036982

订购信息

产品名称产品编号规格价格数量收藏夹

Substance P 抗体 (NC1/34HL)

sc-21715
200 µg/ml
$316.00

Substance P 抗体 (NC1/34HL) AC

sc-21715 AC
500 µg/ml, 25% agarose
$416.00

Substance P 抗体 (NC1/34HL) HRP

sc-21715 HRP
200 µg/ml
$316.00

Substance P 抗体 (NC1/34HL) FITC

sc-21715 FITC
200 µg/ml
$330.00

Substance P 抗体 (NC1/34HL) PE

sc-21715 PE
200 µg/ml
$343.00

Substance P 抗体 (NC1/34HL) Alexa Fluor® 488

sc-21715 AF488
200 µg/ml
$357.00

Substance P 抗体 (NC1/34HL) Alexa Fluor® 546

sc-21715 AF546
200 µg/ml
$357.00

Substance P 抗体 (NC1/34HL) Alexa Fluor® 594

sc-21715 AF594
200 µg/ml
$357.00

Substance P 抗体 (NC1/34HL) Alexa Fluor® 647

sc-21715 AF647
200 µg/ml
$357.00

Substance P 抗体 (NC1/34HL) Alexa Fluor® 680

sc-21715 AF680
200 µg/ml
$357.00

Substance P 抗体 (NC1/34HL) Alexa Fluor® 790

sc-21715 AF790
200 µg/ml
$357.00

Does the anti-SP conjugated to HRP (sc-21715 HRP) work well for indirect ELISAs?

Asked by: Moni
Thank you for your question. HRP conjugated antibody can be used for indirect ELISA as long as you have the proper substrate. https://www.scbt.com/p/3-3-prime-5-5-prime-tetramethylbenzidine-54827-17-7
Answered by: BlakeJ
Date published: 2025-03-13

10 ug sample request for sc-21715 

Asked by: Greg
Thank you for your interest in our sample program. To request a sample please email [email protected]
Answered by: BlakeJ
Date published: 2024-08-28

Looking for an isotype control for the sc-21715 AF488 antibody. That is, rat IgG AlexaFluor 488 conjugated. Is the IgG (H+L), or other? Thank you!

Asked by: MZTMC2023
Thank you for your question . Here is the recommended isotype control : https://www.scbt.com/p/normal-rat-igg-alexa-fluor-488
Answered by: BlakeJ
Date published: 2023-03-21

Hi, Does this antibody can be used in WB?

Asked by: Shuyan
Thank you for your question. This antibody has not yet been tested for Western bloting so that is not a recommended application at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2019-10-31

Which type of antibody is best for IF? Thank you very much.

Asked by: xwang113
This rat monoclonal antibody, sc-21715, is recommended for IF and has been cited in the literature for this application. Please refer to the product citations as a reference.
Answered by: Technical Support
Date published: 2019-01-06

Substance P 抗体 (NC1/34HL): sc-21715 ，这个抗体做western bolt 的molecular weight是多少？急需答复

Asked by: XIAOXIANG
感谢您的提问。Substance P Antibody (NC1/34HL): sc-21715 不推荐用于western blot. Thank you for your question. Substance P Antibody (NC1/34HL): sc-21715 is not recommended for western blot.
Answered by: Technical Support 8
Date published: 2018-12-21

Dear Customer Representive, What is immunogene origin? C-terminus information is only available. Best regards,

Asked by: mustafa4415
Thank you for your question. This is a rat monoclonal where the exact epitope recognized has not yet been determined. Substance P is a processed active peptide with the amino acid sequence below as referenced on the Uniprot sequence page (link provided below as a reference). sp|P20366|58-68 RPKPQQFFGLM https://www.uniprot.org/uniprot/P20366#ptm_processing
Answered by: Technical Support
Date published: 2018-12-03

Can Substance P (NC1/34HL): sc-21715 antibody be used for detection of Substance P of canine origin?

Asked by: cjMara
Substance P is a processed active peptide that is highly conserved among different species so it is likely very possible it will work for canine. To confirm, please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support 5
Date published: 2017-01-25
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Rated 4 out of 5 by from ant-Substance P (NC1/34HL) in gut of pigAt necropsy, intestinal tract of pig were dissected. Sample was washed in NaCl 0.9% and post-fixed by immersion for 7 h in 10% buffered formalin (pH 7.4), dehydrated through a graded series of ethanol and finally embedded in Paraplast X-TRA. Four-µm thick sections were cut in the transversal plane and collected on silane-coated slides. After deparaffinization and rehydratation, slides were incubated overnight at room temperature in a dark moist chamber with ant-Substance P (NC1/34HL) (sc-21715) diluted 1:100 in PBS. After washing in phosphate buffer saline (PBS), sections were incubated for one hour with the secondary antibody Alexa Fluor 488-conjugated Anti-Rat (1:100, Thermo Fisher scientific, Invitrogen, Waltham, MA, USA) in a dark moist chamber. Then the nuclei were counterstained for 10 min with 0.1 μg/mL Hoechst 33,258 (Sigma Aldrich, Milan, Italy). After PBS washing, coverslips were mounted in a drop of Mowiol (Calbiochem, San Diego, CA, USA). Our results clearly demonstrated Substance P-immunopositive neurons in the myenteric plexus of pig, characterized by the presence of green cytoplasmatic immunopositivity (Figure)
Date published: 2022-12-12
Rated 5 out of 5 by from Strong immunostainingStrong immunostaining of Auerbach's and Meissner's plexuses, fine fibres within muscularis externa and also occasional cells within the enterocyte layer
Date published: 2017-02-16
Rated 5 out of 5 by from Cited in PublicationsSubstance P Antibody (NC1/34HL) has been cited in more than a dozen publications!
Date published: 2017-01-17
Rated 4 out of 5 by from IHC publication with paraformaldehyde fixedIHC publication with paraformaldehyde fixed human colon. -SCBT Publication Review
Date published: 2015-05-03
Rated 4 out of 5 by from Publishable IHC data in various human synovialPublishable IHC data in various human synovial tissue -SCBT Publication Review
Date published: 2015-03-13
Rated 4 out of 5 by from Good data with paraffinGood data with paraffin-embedded dog stomach and intestine samples. -SCBT Publication Review
Date published: 2013-09-08
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Substance P 抗体 (NC1/34HL) is rated 4.3 out of 5 by 6.
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