Date published: 2025-9-7

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STRAP 抗体 (E-8): sc-377345

4.5(4)
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说明书
  • STRAP 抗体 E-8 是小鼠单克隆 IgG1 κ,STRAP抗体, 在1篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列1-116位于human物种的STRAP的N-terminus
  • STRAP 抗体 (E-8) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 STRAP; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 抗STRAP抗体(E-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取STRAP (E-8): sc-377345免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是STRAP Antibody (E-8) for WB applications. 的首选辅助检测试剂,这些试剂现与STRAP Antibody (E-8) 打包提供(请参阅下面的订购信息)。

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関連項目

STRAP Antibody (E-8) 是一种IgG1 κ小鼠单克隆STRAP抗体(也称为STRAP抗体),通过WB、IP、IF和ELISA技术可检测来自小鼠、大鼠和人类的STRAP蛋白。STRAP Antibody (E-8) 既可以作为非偶联形式的抗STRAP抗体,也可以作为多种偶联形式的抗STRAP抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Smad蛋白在TGFβ超家族细胞外多肽的细胞内信号传导中起着重要作用。两种Smad蛋白,Smad6和Smad7,作为TGFβ信号传导的拮抗剂。STRAP是TGFβ信号通路的另一个拮抗剂,它特异性地与Smad7相互作用,但不与Smad6相互作用,以协同阻止TGFβ诱导的转录激活。编码人STRAP同源物(如在小鼠中所称)的基因称为unr相互作用蛋白,定位于12p12.3染色体上。在氨基酸水平上,Unr相互作用蛋白与STRAP具有97%的同源性。Unr相互作用蛋白结合unr,unr是一种具有五个冷休克结构域的细胞质RNA结合蛋白,参与RNA翻译。从哺乳动物到酵母的多种物种中都存在STRAP基因,这表明STRAP功能在真核细胞中是进化保守的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

STRAP 抗体 (E-8) 参考文献:

  1. unr是一种细胞质 RNA 结合蛋白,具有五个冷休克结构域,是人类鼻病毒 RNA 翻译内部启动所必需的。  |  Hunt, SL., et al. 1999. Genes Dev. 13: 437-48. PMID: 10049359
  2. STRAP 和 Smad7 在抑制转化生长因子 beta 信号传导方面具有协同作用。  |  Datta, PK. and Moses, HL. 2000. Mol Cell Biol. 20: 3157-67. PMID: 10757800
  3. Smad7和Smad6对转化生长因子β诱导的胚胎肺形态发生抑制有不同的调节作用。  |  Zhao, J., et al. 2000. J Biol Chem. 275: 23992-7. PMID: 10801843
  4. 鉴定 STRAP--转化生长因子-beta 信号传导过程中的新型 WD 结构域蛋白。  |  Datta, PK., et al. 1998. J Biol Chem. 273: 34671-4. PMID: 9856985

订购信息

产品名称产品编号规格价格数量收藏夹

STRAP 抗体 (E-8)

sc-377345
200 µg/ml
RMB2377.00

STRAP (E-8): m-IgG Fc BP-HRP 套装

sc-527598
200 µg Ab; 10 µg BP
RMB2662.00

STRAP (E-8): m-IgG1 BP-HRP 套装

sc-532971
200 µg Ab; 20 µg BP
RMB2662.00

STRAP 抗体 (E-8) AC

sc-377345 AC
500 µg/ml, 25% agarose
RMB3129.00

STRAP 抗体 (E-8) HRP

sc-377345 HRP
200 µg/ml
RMB2377.00

STRAP 抗体 (E-8) FITC

sc-377345 FITC
200 µg/ml
RMB2482.00

STRAP 抗体 (E-8) PE

sc-377345 PE
200 µg/ml
RMB2580.00

STRAP 抗体 (E-8) Alexa Fluor® 488

sc-377345 AF488
200 µg/ml
RMB2685.00

STRAP 抗体 (E-8) Alexa Fluor® 546

sc-377345 AF546
200 µg/ml
RMB2685.00

STRAP 抗体 (E-8) Alexa Fluor® 594

sc-377345 AF594
200 µg/ml
RMB2685.00

STRAP 抗体 (E-8) Alexa Fluor® 647

sc-377345 AF647
200 µg/ml
RMB2685.00

STRAP 抗体 (E-8) Alexa Fluor® 680

sc-377345 AF680
200 µg/ml
RMB2685.00

STRAP 抗体 (E-8) Alexa Fluor® 790

sc-377345 AF790
200 µg/ml
RMB2685.00

What is the recommended starting dilution for Western blot for sc-377345: STRAP (E-8) monoclonal antibody?

Asked by: Germaine
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 3 out of 5 by from good antibodyI tested it in WB with a concentration of 1:1000 on melanoma cell lines A375 and SK-Mel-5. The results was not very good because i obtained more then one band really near to the molecular weight indicated on the datasheet for STRAP so it was not easy to understand which was the right one.
Date published: 2021-12-14
Rated 5 out of 5 by from OttimoHo testato l'anticorpo in western blot (1:500) riscontrando un ottimo funzionamento.
Date published: 2017-12-07
Rated 5 out of 5 by from Nice Western Blot data of STRAP expression in nonNice Western Blot data of STRAP expression in non-transfected and human STRAP transfected 293 whole cell lysates. -SCBT QC
Date published: 2015-02-19
Rated 5 out of 5 by from Produced positive Western Blot data of STRAPProduced positive Western Blot data of STRAP expression in Hep G2 whole cell lysate. -SCBT QC
Date published: 2014-06-10
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STRAP抗体 (E-8) is rated 4.5 out of 5 by 4.
  • y_2025, m_8, d_31, h_8
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