Date published: 2025-9-9

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Stim1 抗体 (CDN3H4): sc-66173

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说明书
  • Stim1 抗体 CDN3H4 是小鼠单克隆 IgG1, 在6篇文献中引用,提供 100 µg/ml
  • 与C-terminal的细胞内部分Stim1的mouse起源
  • 推荐用于 mouse, rat 和 human 来源的Stim1 WB 和 IP检测
  • 关于如何获取Stim1 (CDN3H4): sc-66173免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Stim1 Antibody (CDN3H4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Stim1 Antibody (CDN3H4) 打包提供(请参阅下面的订购信息)。

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Stim1 抗体(CDN3H4)是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过免疫印迹(WB)和免疫沉淀(IP)检测小鼠、大鼠和人样本中的 Stim1。抗 Stim1 抗体 (CDN3H4) 以非结合形式提供,为研究人员提供了研究这种关键蛋白的多功能工具。Stim1或基质相互作用分子1是一种重要的跨膜糖蛋白,位于内质网(ER)中,在ER中Stim1起着钙传感器的作用。Stim1 检测细胞内钙含量变化的能力对分泌和基因转录等各种细胞过程至关重要。当钙储存耗尽时,Stim1 会发生显著的构象变化,从而转位到质膜,在质膜上,Stim1 激活钙释放激活钙(CRAC)通道亚基 TMEM142A/Orai1。这一机制对于维持细胞钙平衡和促进钙流入至关重要,而钙流入是多种生理功能所必需的。此外,Stim1 与 Stim2(已知的 Stim1 介导的贮存操作钙离子进入抑制剂)的相互作用突显了 Stim1 在钙信号通路中的调控作用。Stim1 的影响延伸到肿瘤生长抑制以及基质细胞和造血细胞之间的相互作用,使 Stim1 成为癌症生物学和细胞信号传导研究的一个重要焦点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Stim1 抗体 (CDN3H4) 参考文献:

  1. STIM1 (c)AMPs 促进黑色素生成。  |  Soboloff, J., et al. 2018. EMBO J. 37: PMID: 29449324
  2. STIM1 在神经退行性变中的作用  |  Pascual-Caro, C., et al. 2018. World J Biol Chem. 9: 16-24. PMID: 30568747
  3. STIM1 的结构-功能和下游信号通路。  |  Soboloff, J. and Romanin, C. 2019. Cell Calcium. 80: 101-102. PMID: 30999215
  4. STIM1 是调节肝细胞癌侵袭和转移的代谢检查点。  |  Zhao, H., et al. 2020. Theranostics. 10: 6483-6499. PMID: 32483465
  5. STIM1 在胰腺癌中介导钙依赖性表观遗传重编程  |  Kutschat, AP., et al. 2021. Cancer Res. 81: 2943-2955. PMID: 33436389
  6. STIM1 在肿瘤发生和转移中的作用和机制。  |  Yan, CH., et al. 2023. Yi Chuan. 45: 395-408. PMID: 37194587
  7. TSPAN18 通过保护 STIM1 免受 TRIM32 介导的泛素化而促进前列腺癌的骨转移。  |  Zhou, Q., et al. 2023. J Exp Clin Cancer Res. 42: 195. PMID: 37542345
  8. STIM1 易位到细胞核可保护细胞免受 DNA 损伤。  |  Sanchez-Lopez, I., et al. 2024. Nucleic Acids Res. 52: 2389-2415. PMID: 38224453
  9. 香草酸通过抑制 CA9/STIM1 介导的铁蛋白沉积恢复结肠炎肠上皮细胞的稳态  |  Ni, J., et al. 2024. Pharmacol Res. 202: 107128. PMID: 38438089
  10. STIM1 通过减弱肝细胞癌中的铁变态反应促进对索拉非尼的获得性耐药性。  |  Ren, R., et al. 2024. Genes Dis. 11: 101281. PMID: 39281833

订购信息

产品名称产品编号规格价格数量收藏夹

Stim1 抗体 (CDN3H4)

sc-66173
100 µg/ml
RMB2377.00

Stim1 (CDN3H4): m-IgG Fc BP-HRP 套装

sc-537186
100 µg Ab; 10 µg BP
RMB2662.00

Stim1 (CDN3H4): m-IgGκ BP-HRP 套装

sc-534389
100 µg Ab; 40 µg BP
RMB2662.00

Stim1 (CDN3H4): m-IgG1 BP-HRP 套装

sc-545062
100 µg Ab; 20 µg BP
RMB2662.00

I am using Stim1 (CDN3H4): sc-66173 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Trav11
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24

Bonjour, je voulais savoir quel épitope cet anticorps à pour cible ? Merci d'avance pour la réponse

Asked by: paul2
Thank you for your question. The antibody Stim1 (CDN3H4), sc-66173, was raised against the C-terminal intracellular part of Stim1 of mouse origin. The exact epitope has not been mapped. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2016-11-14
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Rated 3 out of 5 by from not workingA month ago applied for this antibody, after preliminary experiments and experimental optimization, unfortunately we did not get the desired results.
Date published: 2017-06-09
Rated 5 out of 5 by from Good specificity and signal strength in mouseGood specificity and signal strength in mouse Stim1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-03-15
Rated 5 out of 5 by from Very robust signal observed in WB in humanVery robust signal observed in WB in human Stim1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Good antibody for westernGood antibody for western, clean background and specific band in Sol8 and A-10 whole cell lysates and rat thymus and mouse thymus tissue extracts. - SCBT QC
Date published: 2013-06-04
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Stim1 抗体 (CDN3H4) is rated 4.5 out of 5 by 4.
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