Date published: 2025-9-6

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STI1 抗体 (D-6): sc-390203

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说明书
  • STI1 抗体 D-6 是小鼠单克隆 IgG1 κ,STI1抗体, 在2篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列203-453位于human物种的STI1的C-terminus附近
  • STI1 抗体 (D-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 STI1
  • 抗STI1抗体(D-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取STI1 (D-6): sc-390203免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRPm-IgGκ BP-HRP 是 STI1 抗体 (D-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与STI1 抗体 (D-6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

STI1抗体(D-6)是一种IgG1κ小鼠单克隆STI1抗体(也称为STI1抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源STI1蛋白。STI1抗体(D-6)有非偶联的抗STI1抗体形式,以及多种偶联形式的抗STI1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。应激诱导的磷酸蛋白1(STI1)在热休克反应中作为HSP70和HSP90的辅因子。STI1以单体或二聚体的形式存在,这种构象灵活性促进了它在组织HSP70/HSP90中的作用。HSP90作为ATP酶,需要招募客户蛋白和适当的构象才能发挥作用。STI1作为客户蛋白招募的"支架",结合松弛的ADP构象的HSP90,从而抑制加载阶段的ATP周转,并允许正常功能。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

STI1 抗体 (D-6) 参考文献:

  1. 小鼠应激诱导蛋白 1 的辅助伴侣蛋白:过表达, 纯化和表征。  |  van der Spuy, J., et al. 2001. Protein Expr Purif. 21: 462-9. PMID: 11281722
  2. 辅助伴侣 Cdc37p/p50cdc37 对 Hsp90 ATPase 活性的调节。  |  Siligardi, G., et al. 2002. J Biol Chem. 277: 20151-9. PMID: 11916974
  3. 应激诱导蛋白 1 是细胞朊病毒的一种细胞表面配体,可触发神经保护。  |  Zanata, SM., et al. 2002. EMBO J. 21: 3307-16. PMID: 12093732
  4. Hsp90 协同伴侣 Cdc37 和 Sti1 在物理和基因上相互作用。  |  Abbas-Terki, T., et al. 2002. Biol Chem. 383: 1335-42. PMID: 12437126
  5. Sti1 是 Hsp90 ATPase 的一种非竞争性阻抑剂。与之结合可阻止 ATP 酶循环过程中的 N 端二聚化反应。  |  Richter, K., et al. 2003. J Biol Chem. 278: 10328-33. PMID: 12525481
  6. Sti1 是一种新型的 Ssa 蛋白激活剂。  |  Wegele, H., et al. 2003. J Biol Chem. 278: 25970-6. PMID: 12716905
  7. Sti1 和 Cdc37 可以稳定 Hsp90 与蛋白激酶的伴侣复合物。  |  Lee, P., et al. 2004. Mol Biol Cell. 15: 1785-92. PMID: 14742721
  8. PrP 与 STI1 合作调节 PrP 缺失神经细胞系中 SOD 的活性。  |  Sakudo, A., et al. 2005. Biochem Biophys Res Commun. 328: 14-9. PMID: 15670743
  9. 分子克隆和表达一种对转化敏感的人类蛋白,该蛋白含有 TPR 基序,与应激诱导型酵母蛋白 STI1 有相同之处。  |  Honoré, B., et al. 1992. J Biol Chem. 267: 8485-91. PMID: 1569099
  10. 定义Sti1二聚化、与Hsp70和Hsp90相互作用以及体内功能所需的最低片段。  |  Flom, G., et al. 2007. Biochem J. 404: 159-67. PMID: 17300223
  11. Hop/Sti1磷酸化抑制其共肽酶功能。  |  Röhl, A., et al. 2015. EMBO Rep. 16: 240-9. PMID: 25504578
  12. 细胞质辅助伴侣Sti1与线粒体生物发生和形态有关。  |  Hoseini, H., et al. 2016. FEBS J. 283: 3338-52. PMID: 27412066

订购信息

产品名称产品编号规格价格数量收藏夹

STI1 抗体 (D-6)

sc-390203
200 µg/ml
RMB2377.00

STI1 (D-6): m-IgGκ BP-HRP 套装

sc-523449
200 µg Ab, 40 µg BP
RMB2662.00

STI1 (D-6): m-IgG1 BP-HRP 套装

sc-533584
200 µg Ab; 20 µg BP
RMB2662.00

STI1 抗体 (D-6) AC

sc-390203 AC
500 µg/ml, 25% agarose
RMB3129.00

STI1 抗体 (D-6) HRP

sc-390203 HRP
200 µg/ml
RMB2377.00

STI1 抗体 (D-6) FITC

sc-390203 FITC
200 µg/ml
RMB2482.00

STI1 抗体 (D-6) PE

sc-390203 PE
200 µg/ml
RMB2580.00

STI1 抗体 (D-6) Alexa Fluor® 488

sc-390203 AF488
200 µg/ml
RMB2685.00

STI1 抗体 (D-6) Alexa Fluor® 546

sc-390203 AF546
200 µg/ml
RMB2685.00

STI1 抗体 (D-6) Alexa Fluor® 594

sc-390203 AF594
200 µg/ml
RMB2685.00

STI1 抗体 (D-6) Alexa Fluor® 647

sc-390203 AF647
200 µg/ml
RMB2685.00

STI1 抗体 (D-6) Alexa Fluor® 680

sc-390203 AF680
200 µg/ml
RMB2685.00

STI1 抗体 (D-6) Alexa Fluor® 790

sc-390203 AF790
200 µg/ml
RMB2685.00

Can STI1 (D-6): sc-390203 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Professor Griffin
Thank you for your question. Yes, STI1 (D-6): sc-390203 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from GOOD antibodyWestern Blot well in both mouse and human cells, the band is clear.
Date published: 2016-12-20
Rated 5 out of 5 by from Potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was strong detection of the ~63 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested.
Date published: 2016-11-10
Rated 5 out of 5 by from Good Western Blot data of STI1 expressionGood Western Blot data of STI1 expression in A549 whole cell lysate. -SCBT QC
Date published: 2015-05-10
Rated 5 out of 5 by from Satisfactory Western Blot data of STI1 expressionSatisfactory Western Blot data of STI1 expression in non-transfected and mouse STI1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-02-25
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STI1 抗体 (D-6) is rated 4.8 out of 5 by 4.
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