Date published: 2025-9-8

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STEAP 抗体 (B-4): sc-271872

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说明书
  • STEAP 抗体 B-4 是小鼠单克隆 IgG1 κ,STEAP抗体, 在9篇文献中引用,规格为200 µg/ml
  • 免疫human物种的STEAP的氨基酸1-105
  • STEAP 抗体 (B-4) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 STEAP
  • 抗STEAP抗体(B-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取STEAP (B-4): sc-271872免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是STEAP Antibody (B-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与STEAP Antibody (B-4) 打包提供(请参阅下面的订购信息)。

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関連項目

STEAP 抗体 (B-4) 是一种 IgG1 κ 小鼠单克隆 STEAP 抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测人源 STEAP 蛋白。STEAP 抗体 (B-4) 既可以是非偶联的抗 STEAP 抗体形式,也可以是多种偶联形式的抗 STEAP 抗体,包括琼脂糖偶联、辣根过氧化物酶(HRP)偶联、藻红蛋白(PE)偶联、异硫氰酸荧光素(FITC)偶联和多种 Alexa Fluor® 偶联物。前列腺的六跨膜上皮抗原 (STEAP) 在结构上与通道或转运蛋白相似。STEAP 蛋白包含六个潜在的跨膜区域,具有亲水性的氨基和羧基末端结构域。STEAP 蛋白存在于人体前列腺组织中,在癌细胞系(包括前列腺、膀胱、结肠、卵巢和尤因肉瘤)中水平升高。分泌上皮的细胞间连接显示出 STEAP 蛋白的集中水平。小鼠 STEAP 在核苷酸和氨基酸水平上与人 STEAP 有 80% 的同源性。人类 STEAP 基因定位于 7q21 染色体,并编码由 339 个氨基酸组成的蛋白质。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

STEAP 抗体 (B-4) 参考文献:

  1. STEAP:一种在人类前列腺肿瘤中高度表达的前列腺特异性细胞表面抗原。  |  Hubert, RS., et al. 1999. Proc Natl Acad Sci U S A. 96: 14523-8. PMID: 10588738
  2. 小鼠前列腺六膜上皮抗原, 前列腺干细胞抗原和前列腺特异性膜抗原:在转基因腺癌小鼠前列腺癌中高度表达的前列腺特异性细胞表面抗原。  |  Yang, D., et al. 2001. Cancer Res. 61: 5857-60. PMID: 11479226
  3. STEAP 是一种前列腺肿瘤抗原,是人类 CD8+ T 细胞的靶细胞。  |  Alves, PM., et al. 2006. Cancer Immunol Immunother. 55: 1515-23. PMID: 16622681
  4. 检测实体瘤患者血清中的 STEAP mRNA。  |  Valenti, MT., et al. 2009. Cancer Lett. 273: 122-6. PMID: 18793824
  5. STEAP 蛋白家族:多功能氧化还原酶和癌症免疫疗法靶点,具有重叠和独特的细胞功能。  |  Grunewald, TG., et al. 2012. Biol Cell. 104: 641-57. PMID: 22804687
  6. 前列腺六跨膜上皮抗原-1 (STEAP-1)靶向超声成像微泡提高了前列腺癌的体内检测能力。  |  Yuan, Y., et al. 2019. J Ultrasound Med. 38: 299-305. PMID: 30027616
  7. 在诊断过程中利用 STEAP 蛋白家族可提供更全面的前列腺癌预后。  |  Burnell, SEA., et al. 2019. PLoS One. 14: e0220456. PMID: 31393902
  8. 乳腺癌中 STEAP 家族成员的抑瘤作用和预后价值  |  Wu, HT., et al. 2020. Biomed Res Int. 2020: 9578484. PMID: 32802887
  9. 胶质母细胞瘤中 STEAP 家族功能的综合概况及预后预测价值。  |  Chen, H., et al. 2021. J Cell Physiol. 236: 2988-3000. PMID: 32964440
  10. STEAP 家族对胶质瘤患者生存、免疫微环境和治疗反应的预测潜力。  |  Zhao, Z., et al. 2021. Int Immunopharmacol. 101: 108183. PMID: 34649092
  11. 前列腺六膜上皮抗原(STEAP)1 和 2 中的逐步电子转移机制。  |  Chen, K., et al. 2023. Elife. 12: PMID: 37983176
  12. 基于 STEAP 家族的新型肝细胞癌预后特征和治疗指南。  |  Fu, D., et al. 2024. BMC Med Genomics. 17: 16. PMID: 38191397

订购信息

产品名称产品编号规格价格数量收藏夹

STEAP 抗体 (B-4)

sc-271872
200 µg/ml
RMB2377.00

STEAP (B-4): m-IgGκ BP-HRP 套装

sc-522087
200 µg Ab, 40 µg BP
RMB2662.00

STEAP (B-4): m-IgG1 BP-HRP 套装

sc-533517
200 µg Ab; 20 µg BP
RMB2662.00

STEAP 抗体 (B-4) AC

sc-271872 AC
500 µg/ml, 25% agarose
RMB3129.00

STEAP 抗体 (B-4) HRP

sc-271872 HRP
200 µg/ml
RMB2377.00

STEAP 抗体 (B-4) FITC

sc-271872 FITC
200 µg/ml
RMB2482.00

STEAP 抗体 (B-4) PE

sc-271872 PE
200 µg/ml
RMB2580.00

STEAP 抗体 (B-4) Alexa Fluor® 488

sc-271872 AF488
200 µg/ml
RMB2685.00

STEAP 抗体 (B-4) Alexa Fluor® 546

sc-271872 AF546
200 µg/ml
RMB2685.00

STEAP 抗体 (B-4) Alexa Fluor® 594

sc-271872 AF594
200 µg/ml
RMB2685.00

STEAP 抗体 (B-4) Alexa Fluor® 647

sc-271872 AF647
200 µg/ml
RMB2685.00

STEAP 抗体 (B-4) Alexa Fluor® 680

sc-271872 AF680
200 µg/ml
RMB2685.00

STEAP 抗体 (B-4) Alexa Fluor® 790

sc-271872 AF790
200 µg/ml
RMB2685.00

Can this product be used for western blotting to detect STEAP1B?

Asked by: nh27
Thank you for your question. STEAP Antibody (B-4): sc-271872, recommended for detection of STEAP1 protein only, as follows: https://www.uniprot.org/uniprotkb/Q9UHE8/entry#names_and_taxonomy Please refer to it, Thank you.
Answered by: Jiajun Z
Date published: 2024-03-28

这个抗体是针对于STEAP1?还是整个STEAP家族?

Asked by: linghan
感谢您的提问。STEAP 抗体 (B-4): sc-271872 检测 STEAP1. Thank you for your question. STEAP antibody (B-4): sc-271872 detects STEAP1.
Answered by: Technical Service SH
Date published: 2023-11-09

Is this antibody(sc-271872;STEAP antibody) suitable for immunostaining and what is the recommended starting dilution? If not, is there another STEAP1 antibody suitable for IF?

Asked by: April123
Yes this is approved for use in immunostaining applications. We recommend a starting dilution of 1:50. For more information, please refer to our immunohistochemistry protocol.
Answered by: Dallas Tech Service
Date published: 2021-04-07

Is this antibody(sc-271872;STEAP antibody) suitable for immunostaining? If so, what is the recommended starting dilution?

Asked by: nh27
Thank you for your question. This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates! We recommend a starting dilution of 1:50 (range 1:50-1:500).
Answered by: Technical Support
Date published: 2020-09-13

What is the recommended starting dilution for Western blot for sc-271872: STEAP (B-4) monoclonal antibody?

Asked by: Professor Griffin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Nice antibody for western blotWe used this antibody for expression detection of STEAP by western blot. We were able to get nice bands around the correct molecular weight
Date published: 2017-02-03
Rated 5 out of 5 by from Produced nice Western blot data of STEAPProduced nice Western blot data of STEAP expression in LNCaP whole cell lysate. -SCBT QC
Date published: 2013-07-01
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STEAP抗体 (B-4) is rated 5.0 out of 5 by 2.
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