Date published: 2025-9-9
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SRC-1 Antibody (1135/H4) - Western Blotting - Image 10474
SRC-1 抗体 (1135/H4): sc-32789. KNRK 核提取物 (A) 和 COS E2 全细胞裂解液 (B) 中 SRC-1 表达的 Western 印迹分析.
SRC-1 Antibody (1135/H4) - Western Blotting - Image 10474
SRC-1 抗体 (1135/H4): sc-32789. KNRK 核提取物 (A) 和 COS E2 全细胞裂解液 (B) 中 SRC-1 表达的 Western 印迹分析.
SRC-1 Antibody (1135/H4) - Western Blotting - Image 385472
SRC-1 抗体 (1135/H4): sc-32789. KNRK 核提取物中 SRC-1 表达的近红外 Western 印迹分析. 使用 UltraCruz® 阻断试剂: sc-516214. 使用的检测试剂: m-IgGκ BP-CFL 680: sc-516180.
SRC-1 Antibody (1135/H4) - Western Blotting - Image 412549
SRC-1 抗体 (1135/H4)：sc-32789。K-562 全细胞裂解液中 SRC-1 表达的 Western 印迹分析。所用检测试剂：m-IgG Fc BP-HRP：sc-525409。
SRC-1 抗体 (1135/H4): sc-32789. KNRK 核提取物 (A) 和 COS E2 全细胞裂解液 (B) 中 SRC-1 表达的 Western 印迹分析.

SRC-1 抗体 (1135/H4): sc-32789

4.1(10)
寫評論提問

说明书
  • SRC-1 抗体 1135/H4 是小鼠单克隆 IgG1 κ，SRC-1抗体, 在27篇文献中引用，规格为200 µg/ml
  • 针对SRC-1融合蛋白的氨基酸477-947
  • SRC-1 抗体 (1135/H4) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 SRC-1
  • 抗SRC-1抗体（1135/H4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-32789 X, 200 µg/0.1 ml)
  • 关于如何获取SRC-1 (1135/H4): sc-32789的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 SRC-1 抗体 (1135/H4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SRC-1 抗体 (1135/H4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

SRC-1抗体（1135/H4）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类来源的SRC-1蛋白。抗SRC-1抗体（1135/H4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。SRC-1蛋白（又称类固醇受体协同因子-1）在调节基因表达方面发挥着至关重要的作用，它充当核激素受体的协同因子，而核激素受体对于调节类固醇激素、甲状腺激素和维甲酸的作用至关重要。SRC-1主要位于细胞核中，与转录因子相互作用，从而增强靶基因的转录。SRC-1的核定位至关重要，因为SRC-1能够根据激素信号精确调节基因表达，从而影响新陈代谢、发育和繁殖等多种生理过程。SRC-1会经历翻译后修饰，包括磷酸化，从而调节其活性以及与其他蛋白的相互作用，进一步凸显了SRC-1在细胞信号通路中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SRC-1 抗体 (1135/H4) 参考文献:

  1. SRC-1基因敲除对APP/PS1小鼠的淀粉样β沉积无影响  |  Wu, Q., et al. 2020. Front Aging Neurosci. 12: 145. PMID: 32625077
  2. 疏水标签介导的转录辅激活子 SRC-1 降解。  |  Choi, SR., et al. 2021. Int J Mol Sci. 22: PMID: 34203850
  3. Src-1和SP2促进鼻咽癌的增殖和上皮-间质转化。  |  Zhang, J., et al. 2021. Open Med (Wars). 16: 1061-1069. PMID: 34307888
  4. 核受体辅助激活因子SRC-1通过增强GLI2-介导的刺猬信号促进结直肠癌的进展。  |  Guo, P., et al. 2022. Oncogene. 41: 2846-2859. PMID: 35418691
  5. 类固醇受体辅激活剂-1（SRC-1）通过 NF-κB 激活 VEGFC 促进胃癌细胞转移  |  Zhao, X., et al. 2022. Crit Rev Eukaryot Gene Expr. 32: 21-29. PMID: 35695662
  6. 靶向核受体辅激活子 SRC-1 通过降低 PD-L1 的转录和蛋白稳定性防止结直肠癌免疫逃逸  |  Hong, Y., et al. 2024. Adv Sci (Weinh). 11: e2310037. PMID: 38953362
  7. 类固醇激素受体超家族辅助激活剂的序列和特征。  |  Oñate, SA., et al. 1995. Science. 270: 1354-7. PMID: 7481822
  8. 核荷尔蒙受体基因超家族。  |  Ribeiro, RC., et al. 1995. Annu Rev Med. 46: 443-53. PMID: 7598477
  9. GRIP1 是一种新型小鼠蛋白，在酵母中可作为类固醇受体激素结合域的转录辅激活剂。  |  Hong, H., et al. 1996. Proc Natl Acad Sci U S A. 93: 4948-52. PMID: 8643509
  10. 转录协同激活因子 p/CIP 与 CBP 结合并介导核受体功能。  |  Torchia, J., et al. 1997. Nature. 387: 677-84. PMID: 9192892
  11. RAC3 是一种与 SRC-1 和 TIF2 有关的类固醇/核受体相关辅激活子。  |  Li, H., et al. 1997. Proc Natl Acad Sci U S A. 94: 8479-84. PMID: 9238002
  12. AIB1，一种在乳腺癌和卵巢癌中扩增的类固醇受体辅激活剂。  |  Anzick, SL., et al. 1997. Science. 277: 965-8. PMID: 9252329

订购信息

产品名称产品编号规格价格数量收藏夹

SRC-1 抗体 (1135/H4)

sc-32789
200 µg/ml
RMB2377.00

SRC-1 (1135/H4): m-IgG Fc BP-HRP 套装

sc-528473
200 µg Ab; 10 µg BP
RMB2662.00

SRC-1 (1135/H4): m-IgGκ BP-HRP 套装

sc-520862
200 µg Ab, 40 µg BP
RMB2662.00

SRC-1 (1135/H4): m-IgG1 BP-HRP 套装

sc-542951
200 µg Ab; 20 µg BP
RMB2662.00

SRC-1 抗体 (1135/H4) X

sc-32789 X
200 µg/0.1 ml
RMB2377.00

SRC-1 抗体 (1135/H4) AC

sc-32789 AC
500 µg/ml, 25% agarose
RMB3129.00

SRC-1 抗体 (1135/H4) HRP

sc-32789 HRP
200 µg/ml
RMB2377.00

SRC-1 抗体 (1135/H4) FITC

sc-32789 FITC
200 µg/ml
RMB2482.00

SRC-1 抗体 (1135/H4) PE

sc-32789 PE
200 µg/ml
RMB2580.00

SRC-1 抗体 (1135/H4) Alexa Fluor® 488

sc-32789 AF488
200 µg/ml
RMB2685.00

SRC-1 抗体 (1135/H4) Alexa Fluor® 546

sc-32789 AF546
200 µg/ml
RMB2685.00

SRC-1 抗体 (1135/H4) Alexa Fluor® 594

sc-32789 AF594
200 µg/ml
RMB2685.00

SRC-1 抗体 (1135/H4) Alexa Fluor® 647

sc-32789 AF647
200 µg/ml
RMB2685.00

SRC-1 抗体 (1135/H4) Alexa Fluor® 680

sc-32789 AF680
200 µg/ml
RMB2685.00

SRC-1 抗体 (1135/H4) Alexa Fluor® 790

sc-32789 AF790
200 µg/ml
RMB2685.00

is this antibody to SRC-1 good for ChIP seq

Asked by: christak
Thank you for your question. We recommend sc-32789 X for Chip assay. https://www.scbt.com/resources/protocols/chromatin-immunoprecipitation-assays
Answered by: BlakeJ
Date published: 2023-05-31

Hi , I have ordered this antibody for protein src ( sc-32789), why it's molecular weight is so high (Mol.wt- 160 Kda), as compared to molecular weight of its phosphorylated form (p-src mol wt- 60 Kda). I have been looking for total protein form of p-src

Asked by: RUPA
Thank you for your question. This antibody is specific for SRC-1 (for steroid receptor coactivator-1, also designated NCoA-1). This protein has a predicted molecular weight of 160 kDa. You are comparing this to a different protein/gene, p-src, or p-c-Src, which is part of the Src gene family of membrane-associated tyrosine protein kinases. If you are looking for a total antibody against this latter target, then I would recommend a different antibody, c-Src Antibody (B-12): sc-8056.
Answered by: Technical Support
Date published: 2019-01-26

When should the TransCruz reagent sc-32789 X be used instead of the standard form sc-32789?

Asked by: TinTin
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 抗体挺好抗体挺不错哒，杂带少，特异性高，已经订了很多个santa的抗体，使用过程中还没有出现过什么问题，挺放心哒
Date published: 2025-03-03
Rated 5 out of 5 by from Quite clear band for wb of rat protein1:250 diluted for wb of rat protein,the band is special and strong.
Date published: 2017-08-28
Rated 5 out of 5 by from functionalA year ago to buy the trial equipment, dilution is too small.
Date published: 2017-07-05
Rated 5 out of 5 by from specific resultsSRC-1 Antibody gives clean and specific results in WB.
Date published: 2017-04-21
Rated 5 out of 5 by from suitable for WBSuitalbe for WB detect of SRC-1,very good binding the target protein, shows strong signal and no background.
Date published: 2017-02-12
Rated 2 out of 5 by from The effect is very poorLook at the picture below it, the result is very poor. I made an experiment according to the instruction manual. Hope to get technical support from Santa company.
Date published: 2016-12-14
Rated 1 out of 5 by from Not very well for western blot.This antibody can detect a strong band at predicted size (~160kDa) in HepG2 cells. However, the signal may be non-specific.
Date published: 2016-11-07
Rated 4 out of 5 by from Good results in WBGood results in WB
Date published: 2015-03-31
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SRC-1 抗体 (1135/H4) is rated 4.1 out of 5 by 10.
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