Date published: 2025-9-7
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SR Antibody (1H4) - Western Blotting - Image 352712
SR 抗体 (1H4): sc-13509. HL-60 (A), K-562 (B), HeLa (C), PC-3 (D) 和 MOLT-4 (E) 核提取物中 SR 表达的 Western 印迹分析.
SR 抗体 (1H4): sc-13509. HL-60 (A), K-562 (B), HeLa (C), PC-3 (D) 和 MOLT-4 (E) 核提取物中 SR 表达的 Western 印迹分析.

SR 抗体 (1H4): sc-13509

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说明书
  • SR 抗体 1H4 是小鼠单克隆 IgG1 κ，SR抗体, 在20篇文献中引用，规格为200 µg/ml
  • 针对全长SR，Xenopus起源
  • SR 抗体 (1H4) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human, 和 Xenopus 来源的 SR RNA processing factors, including SRp75, SRp55, SRp40, SRp30a/b and SRp20; 也和以下物种反应，包括： bovine and avian
  • 抗SR抗体（1H4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SR (1H4): sc-13509的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 SR 抗体 (1H4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SR 抗体 (1H4) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 SR RNA Splicing Factor 抗体包括 SRm160, SRm300, SR-1D, SR-1E, SR-2A, SR-2B, SR-2C, SR-3A, SR-3B 和 SR-4

SR抗体（1H4）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）和酶联免疫吸附测定（ELISA）检测人类来源的SR蛋白。SR (1H4) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合型。SR 蛋白家族在预 mRNA 剪接中起着至关重要的作用，而预 mRNA 剪接对于 mRNA 的准确处理和基因表达的调控至关重要。SR蛋白主要位于细胞核中，与特定的RNA序列结合，称为剪接增强子，对识别剪接位点和控制可变剪接至关重要。丝氨酸/精氨酸富集（SR）剪接因子（如SC35和SRp40）的存在对构象剪接至关重要，而其他成员（如SF2/ASF和SRp20）则在细胞核和细胞质之间穿梭，促进mRNA运输。SR蛋白的复杂平衡以及与其他剪接因子（包括异质核糖核蛋白（hnRNP）家族成员）的相互作用，凸显了其在剪接事件调节中的重要性，这使得SR（1H4）单克隆抗体成为研究人类细胞中基因表达和剪接机制的研究人员的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SR 抗体 (1H4) 参考文献:

  1. 剪接因子缔合作用蛋白 p32 通过抑制 ASF/SF2 RNA 结合和磷酸化来调节 RNA 剪接。  |  Petersen-Mahrt, SK., et al. 1999. EMBO J. 18: 1014-24. PMID: 10022843
  2. 前 mRNA 剪接增强子的选择和表征：新型 SR 蛋白特异性增强子序列的鉴定。  |  Schaal, TD. and Maniatis, T. 1999. Mol Cell Biol. 19: 1705-19. PMID: 10022858
  3. 剪接因子 9G8 和 SRp20 通过不同的特异性增强子反式激活剪接。  |  Cavaloc, Y., et al. 1999. RNA. 5: 468-83. PMID: 10094314
  4. 丝氨酸/精氨酸富集剪接因子4的生理和病理功能及相关疾病。  |  Tan, W., et al. 2018. Biomed Res Int. 2018: 3819719. PMID: 29789787
  5. 丝氨酸-精氨酸富集剪接因子（SR）家族蛋白在发育和疾病中的非经典功能。  |  Wagner, RE. and Frye, M. 2021. Bioessays. 43: e2000242. PMID: 33554347
  6. 丝氨酸精氨酸富集剪接因子（SRSF7）与组蛋白甲基转移酶KMT5a协同作用，通过激活IRF7的转录物来促进I型干扰素的反应。  |  Scott, HM., et al. 2023. bioRxiv.. PMID: 37503164
  7. 将选择性剪接作为新的癌症免疫疗法--丝氨酸精氨酸富集剪接因子（SRSF1）的磷酸化由SR蛋白激酶1（SRPK1）调节PD1的选择性剪接，产生可溶性拮抗异构体，防止T细胞衰竭。  |  Wahid, M., et al. 2023. Cancer Immunol Immunother. 72: 4001-4014. PMID: 37973660
  8. 丝氨酸/精氨酸富集剪接因子7通过激活Irf7转录来促进I型干扰素的反应。  |  Scott, HM., et al. 2024. Cell Rep. 43: 113816. PMID: 38393946
  9. hnRNP A1 和其他 hnRNP A/B 蛋白保守结构域的功能。  |  Mayeda, A., et al. 1994. EMBO J. 13: 5483-95. PMID: 7957114
  10. 单个 SR 蛋白对不同前 mRNA 进行剪接的特异性承诺。  |  Fu, XD. 1993. Nature. 365: 82-5. PMID: 8361546
  11. 剪接因子 SRp20 可改变自身 mRNA 的剪接，而 ASF/SF2 可颉颃这种调节作用。  |  Jumaa, H. and Nielsen, PJ. 1997. EMBO J. 16: 5077-85. PMID: 9305649
  12. 一个特定的 SR 蛋白子集在细胞核和细胞质之间不断穿梭。  |  Cáceres, JF., et al. 1998. Genes Dev. 12: 55-66. PMID: 9420331

订购信息

产品名称产品编号规格价格数量收藏夹

SR 抗体 (1H4)

sc-13509
200 µg/ml
RMB2377.00

SR (1H4): m-IgG Fc BP-HRP 套装

sc-526547
200 µg Ab; 10 µg BP
RMB2662.00

SR (1H4): m-IgG1 BP-HRP 套装

sc-531920
200 µg Ab; 20 µg BP
RMB2662.00

SR 抗体 (1H4) AC

sc-13509 AC
500 µg/ml, 25% agarose
RMB3129.00

SR 抗体 (1H4) HRP

sc-13509 HRP
200 µg/ml
RMB2377.00

SR 抗体 (1H4) FITC

sc-13509 FITC
200 µg/ml
RMB2482.00

SR 抗体 (1H4) PE

sc-13509 PE
200 µg/ml
RMB2580.00

SR 抗体 (1H4) Alexa Fluor® 488

sc-13509 AF488
200 µg/ml
RMB2685.00

SR 抗体 (1H4) Alexa Fluor® 546

sc-13509 AF546
200 µg/ml
RMB2685.00

SR 抗体 (1H4) Alexa Fluor® 594

sc-13509 AF594
200 µg/ml
RMB2685.00

SR 抗体 (1H4) Alexa Fluor® 647

sc-13509 AF647
200 µg/ml
RMB2685.00

SR 抗体 (1H4) Alexa Fluor® 680

sc-13509 AF680
200 µg/ml
RMB2685.00

SR 抗体 (1H4) Alexa Fluor® 790

sc-13509 AF790
200 µg/ml
RMB2685.00

SR Antibody (1H4, sc-13509): Has someone observed whether this antibody is cross-reactive with mouse?

Asked by: raheld
Thank you for your question. PMID 28689656 cites use in murine P19 cells, so there does seem to be cross-reactivity with at least some murine samples.
Answered by: Tech Support Europe
Date published: 2023-06-02

Hi, does the antibody detect SR proteins or phopsphorylated SR protein. Where can I get more information on this in the data sheet? Thanks

Asked by: Riddhi
Thank you for your question. This antibody recognises both phosphorylated and non-phosphorylated protein.
Answered by: Tech Support Europe
Date published: 2022-08-03

Can SR (1H4): sc-13509 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, SR (1H4): sc-13509 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 15
Date published: 2022-04-13
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Rated 5 out of 5 by from an excellent antibody for both WB and IFThis antibody works well for WB (1:500 dilution, 40 ug HeLa lysate) and IF (1:400 dilution, formaldehyde-fixed HeLa)
Date published: 2017-07-11
Rated 5 out of 5 by from Wow !! Superb productOutstanding WB, IF and IHC staining data; as good as it gets !!
Date published: 2017-02-02
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of SR expression in HL-60, K-562, HeLa, PC-3 and MOLT-4 nuclear extracts.
Date published: 2017-01-11
Rated 5 out of 5 by from Good staining in IF in human CaluGood staining in IF in human Calu-6 cells. -SCBT Publication Review
Date published: 2015-03-22
Rated 5 out of 5 by from Produced exceptional immunoperoxidase nuclearProduced exceptional immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-03-10
Rated 5 out of 5 by from Strong pulldown in IP in human HEPStrong pulldown in IP in human HEP-2 cells. -SCBT Publication Review
Date published: 2015-02-20
Rated 5 out of 5 by from Strong staining in IHC in human cervical tissueStrong staining in IHC in human cervical tissue. -SCBT Publication Review
Date published: 2015-02-09
Rated 5 out of 5 by from Strong band in WB in BJAB and DT40 wholeStrong band in WB in BJAB and DT40 whole cell lysates. -SCBT QC
Date published: 2015-01-08
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SR 抗体 (1H4) is rated 5.0 out of 5 by 8.
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