Date published: 2025-9-7

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SR-4 抗体 (G-3): sc-376158

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说明书
  • SR-4 抗体 G-3 是小鼠单克隆 IgG1 κ,SR-4抗体, 在5篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列156-284位于human物种的SR-4的内部区域内
  • SR-4 抗体 (G-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 SR-4
  • 抗SR-4抗体(G-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SR-4 (G-3): sc-376158免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 SR-4 抗体 (G-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SR-4 抗体 (G-3) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

SR-4抗体(G-3)是一种IgG1 κ小鼠单克隆SR-4抗体(也称为HTR4抗体、5HT4R抗体、5-羟色胺受体4抗体、5-HT4抗体、5-羟色胺(5-羟色胺)受体4抗体、G蛋白偶联5-羟色胺受体4转录变体B2抗体、心脏5-HT4受体抗体、5-羟色胺受体4抗体、5-HT4R抗体或5-HT-4抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的SR-4蛋白。SR-4抗体(G-3)既有非偶联型抗SR-4抗体形式,也有多种偶联型抗SR-4抗体形式,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。5-羟色胺(也称为5-羟色胺或5-HT)是一种分子,具有神经递质、激素和有丝分裂原的功能,主要在肠道、血小板和中枢神经系统(CNS)中表达。在中枢神经系统中,5-羟色胺调节多种过程,包括焦虑、睡眠、食欲、行为和药物滥用。在血小板和肠道中,5-羟色胺分别在心血管功能和胃肠道蠕动中起主要作用。5-羟色胺通过几种G蛋白偶联受体介导其作用,这些受体被称为5-HT受体或SR受体。SR-3是一种配体门控离子通道,而所有其他已知的5-羟色胺受体亚型都是G蛋白偶联受体。编码SR-3的基因位于人类染色体11q23.1-q23.2上。SR-4在中枢和周围神经系统中介导广泛的作用。编码SR-4的基因位于人类染色体5q31-q33上。SR-7属于G蛋白偶联受体超家族。编码SR-7的基因位于人类染色体10q21-q24上。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SR-4 抗体 (G-3) 参考文献:

  1. 心脏发育需要羟色胺 2B 受体。  |  Nebigil, CG., et al. 2000. Proc Natl Acad Sci U S A. 97: 9508-13. PMID: 10944220
  2. 利用基因敲除小鼠剖析血清素系统在神经精神疾病中的作用。  |  Gingrich, JA. and Hen, R. 2001. Psychopharmacology (Berl). 155: 1-10. PMID: 11374326
  3. 药理受体的定义。  |  Kenakin, TP., et al. 1992. Pharmacol Rev. 44: 351-62. PMID: 1438521
  4. 血清素门控离子通道 5HT3 受体的初级结构和功能表达。  |  Maricq, AV., et al. 1991. Science. 254: 432-7. PMID: 1718042
  5. 5-羟色胺受体4激动剂莫沙必利对人类胃适应性的影响。  |  Amano, T., et al. 2015. Neurogastroenterol Motil. 27: 1303-9. PMID: 26303048
  6. 将 5HT7 受体基因 (HTR7) 分配到染色体 10q 并排除与妥瑞症的遗传联系。  |  Gelernter, J., et al. 1995. Genomics. 26: 207-9. PMID: 7601444
  7. 激活 cAMP 形成的高亲和性血清素受体(5-HT7)的分子克隆, 表征和定位。  |  Ruat, M., et al. 1993. Proc Natl Acad Sci U S A. 90: 8547-51. PMID: 8397408
  8. 将小鼠 Htr3 受体基因的人类同源物分配到染色体 11q23.1-q23.2 上。  |  Weiss, B., et al. 1995. Genomics. 29: 304-5. PMID: 8530095
  9. 中枢 5-HT4 受体  |  Eglen, RM., et al. 1995. Trends Pharmacol Sci. 16: 391-8. PMID: 8578609
  10. 通过原位杂交将5-羟色胺受体(HTR4)分配到人类5号染色体q31-->q33带。  |  Claeysen, S., et al. 1997. Cytogenet Cell Genet. 78: 133-4. PMID: 9371406
  11. 血清素通过 5-HT2A 受体诱导早期反应基因前列腺素 G/H 合成酶-2和egr-1的信号通路。  |  Goppelt-Struebe, M. and Stroebel, M. 1998. J Cell Physiol. 175: 341-7. PMID: 9572479
  12. 5-HT3B 亚基是决定血清素受体功能的主要因素。  |  Davies, PA., et al. 1999. Nature. 397: 359-63. PMID: 9950429

订购信息

产品名称产品编号规格价格数量收藏夹

SR-4 抗体 (G-3)

sc-376158
200 µg/ml
RMB2377.00

SR-4 (G-3): m-IgG Fc BP-HRP 套装

sc-527516
200 µg Ab; 10 µg BP
RMB2662.00

SR-4 (G-3): m-IgG1 BP-HRP 套装

sc-532889
200 µg Ab; 20 µg BP
RMB2662.00

SR-4 抗体 (G-3) AC

sc-376158 AC
500 µg/ml, 25% agarose
RMB3129.00

SR-4 抗体 (G-3) HRP

sc-376158 HRP
200 µg/ml
RMB2377.00

SR-4 抗体 (G-3) FITC

sc-376158 FITC
200 µg/ml
RMB2482.00

SR-4 抗体 (G-3) PE

sc-376158 PE
200 µg/ml
RMB2580.00

SR-4 抗体 (G-3) Alexa Fluor® 488

sc-376158 AF488
200 µg/ml
RMB2685.00

SR-4 抗体 (G-3) Alexa Fluor® 546

sc-376158 AF546
200 µg/ml
RMB2685.00

SR-4 抗体 (G-3) Alexa Fluor® 594

sc-376158 AF594
200 µg/ml
RMB2685.00

SR-4 抗体 (G-3) Alexa Fluor® 647

sc-376158 AF647
200 µg/ml
RMB2685.00

SR-4 抗体 (G-3) Alexa Fluor® 680

sc-376158 AF680
200 µg/ml
RMB2685.00

SR-4 抗体 (G-3) Alexa Fluor® 790

sc-376158 AF790
200 µg/ml
RMB2685.00

I am using SR-4 (G-3): sc-376158 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Optimal antibodyAntibody diluition 1:1000 in 5%BSA in TBS-T solution. western blot human vaginal tissue
Date published: 2024-12-17
Rated 1 out of 5 by from 不好用,免疫组化一塌糊涂完全无法显色,同时对于阳性对照都无法发色。而且完整协议中无法具体显示实验的细节,wb图片对于结果很差。
Date published: 2023-06-01
Rated 1 out of 5 by from Question Mark?I received samples of this antibody with Lot #G1217 and had a very good response. I received my new antibody vial which had a Lot #F0920 and got nothing with same dilutions. I don't have time to money to keep using sample and supplies to troubleshoot vials of antibody. Currently in a conflict with SC Technical. Interesting how SC offered a datasheet with a western blot image for Lot #G1217 by which my blots looked similar to and responded to, but the datasheet for Lot #F0920 had no image for western blot. Ironically I got no results also.
Date published: 2020-07-07
Rated 1 out of 5 by from Bad resultsThe Ab doesn't work well with IF and IHC. We use a standard protocol, but we can see anything in our rat's slices.
Date published: 2018-03-07
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic stainingGreat immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human upper stomach tissue. -SCBT QC
Date published: 2015-05-07
Rated 5 out of 5 by from Robust signal in WB in SW480 whole cell lysateRobust signal in WB in SW480 whole cell lysate. -SCBT QC
Date published: 2015-01-05
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SR-4 抗体 (G-3) is rated 2.8 out of 5 by 6.
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