Date published: 2025-9-7
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SR-2A Antibody (A-4) - Western Blotting - Image 148858
SR-2A 抗体 (A-4): sc-166775. HT-1080 (A), HEK293 (B), PC-3 (C), JAR (D), U-87 MG (E) 和 T24 (F) 全细胞裂解液中 SR-2A 表达的 Western 印迹分析.
SR-2A 抗体 (A-4): sc-166775. HT-1080 (A), HEK293 (B), PC-3 (C), JAR (D), U-87 MG (E) 和 T24 (F) 全细胞裂解液中 SR-2A 表达的 Western 印迹分析.

SR-2A 抗体 (A-4): sc-166775

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说明书
  • SR-2A 抗体 A-4 是小鼠单克隆 IgG1 κ，SR-2A抗体, 在36篇文献中引用，规格为200 µg/ml
  • 针对SR-2A的human起源的N-terminal细胞外域内的氨基酸1-75
  • SR-2A 抗体 (A-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 serotonin 2A receptor (5-HT-2)
  • 抗SR-2A抗体（A-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SR-2A (A-4): sc-166775的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 SR-2A 抗体 (A-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SR-2A 抗体 (A-4) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 SR 抗体包括 SR-1D, SR-1E, SR-2A, SR-2B, SR-2C, SR-3A, SR-3B 和 SR-4

SR-2A抗体（A-4）是一种IgG1 κ小鼠单克隆SR-2A抗体（也称为HTR2A抗体、5HT2A抗体、5-羟色胺受体2A抗体、5-羟色胺（血清素）受体2A抗体、G蛋白偶联5-HT2A抗体、HTR2抗体、血清素5-HT-2A受体抗体、血清素受体2A抗体、5-HT2受体抗体或5-HT-2A抗体），可通过WB, IP, IF, IHC(P) 和 ELISA检测小鼠、大鼠和人类来源的SR-2A蛋白。SR-2A抗体（A-4）既有非偶联的抗SR-2A抗体形式，也有多种偶联形式的抗SR-2A抗体，包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®偶联物。血清素（也被称为5-羟色胺或5-HT）是一种分子，具有神经递质、激素和有丝分裂原的功能，主要在肠道、血小板和中枢神经系统（CNS）中表达。在中枢神经系统中，血清素调节几个过程，包括焦虑、睡眠、食欲、行为和药物滥用。在血小板和肠道中，血清素分别在心血管功能和胃肠道蠕动中起主要作用。血清素通过几种G蛋白偶联受体介导其作用，这些受体被称为5-HT受体或SR受体。SR-2受体由三种亚型组成，SR-2A、SR-2B和SR-2C，它们激活磷脂酶C并释放细胞内储存的钙以响应血清素。SR-2A在气管平滑肌收缩、支气管收缩和调节醛固酮产生方面具有特定作用，也被认为在几种精神疾病中发挥作用，包括抑郁症和精神分裂症。SR-2B在胚胎和成人心血管组织、肠道和大脑中表达，在心脏疾病的病理学中发挥重要作用。SR-2C被认为可以介导非典型抗精神病药物的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SR-2A 抗体 (A-4) 参考文献:

  1. 大鼠大脑中血清素（2A）（5HT（2A））受体的细胞定位。  |  Xu, T. and Pandey, SC. 2000. Brain Res Bull. 51: 499-505. PMID: 10758340
  2. 心脏发育需要羟色胺 2B 受体。  |  Nebigil, CG., et al. 2000. Proc Natl Acad Sci U S A. 97: 9508-13. PMID: 10944220
  3. 非典型抗精神病药物对人类 5- 羟色胺 2C受体的反向激动活性。  |  Herrick-Davis, K., et al. 2000. J Pharmacol Exp Ther. 295: 226-32. PMID: 10991983
  4. 5-HT 在调节大脑-垂体-肾上腺轴中的作用：5-HT 对肾上腺皮质细胞的影响。  |  Contesse, V., et al. 2000. Can J Physiol Pharmacol. 78: 967-83. PMID: 11149386
  5. 利用基因敲除小鼠剖析血清素系统在神经精神疾病中的作用。  |  Gingrich, JA. and Hen, R. 2001. Psychopharmacology (Berl). 155: 1-10. PMID: 11374326
  6. 5-羟色胺受体2A基因中的rs6313多态性与多症状原发性夜间遗尿症之间的关联。  |  Wei, CC., et al. 2010. J Clin Lab Anal. 24: 371-5. PMID: 21089166
  7. 色氨酸羟化酶 1/甲胺氧化酶-A/5-羟色胺/5-羟色胺受体 2A/2B/2C 轴的调节作用可调控胆汁淤积过程中的胆汁增殖和肝纤维化  |  Kyritsi, K., et al. 2020. Hepatology. 71: 990-1008. PMID: 31344280
  8. 5-羟色胺受体2A在药物滥用性头痛的病理生理学中的作用。  |  Zheng, Z., et al. 2021. J Pain Res. 14: 453-461. PMID: 33623427
  9. 5-羟色胺受体2A基因（rs6313和rs4941573）多态性与睡眠磨牙症之间的关联：荟萃分析。  |  Motahari, P., et al. 2023. Sleep Sci. 16: 248-255. PMID: 37425969
  10. 5-羟色胺受体2A基因变异rs6311和rs6313与伊朗库尔德人阻塞性睡眠呼吸暂停之间的关联。  |  Abdolsamadi, M., et al. 2024. Genet Test Mol Biomarkers. 28: 159-164. PMID: 38657123
  11. 豚鼠气管中的收缩羟色胺-2A 受体信号转导：蛋白激酶 C 和细胞外及细胞内钙的重要性，而非磷脂水解的重要性。  |  Watts, SW., et al. 1994. J Pharmacol Exp Ther. 271: 832-44. PMID: 7965803
  12. 血清素通过 5-HT2A 受体诱导早期反应基因前列腺素 G/H 合成酶-2和egr-1的信号通路。  |  Goppelt-Struebe, M. and Stroebel, M. 1998. J Cell Physiol. 175: 341-7. PMID: 9572479

订购信息

产品名称产品编号规格价格数量收藏夹

SR-2A 抗体 (A-4)

sc-166775
200 µg/ml
RMB2377.00

SR-2A (A-4): m-IgG Fc BP-HRP 套装

sc-529046
200 µg Ab; 10 µg BP
RMB2662.00

SR-2A (A-4): m-IgGκ BP-HRP 套装

sc-521740
200 µg Ab, 40 µg BP
RMB2662.00

SR-2A (A-4): m-IgG1 BP-HRP 套装

sc-543288
200 µg Ab; 20 µg BP
RMB2662.00

SR-2A 抗体 (A-4) AC

sc-166775 AC
500 µg/ml, 25% agarose
RMB3129.00

SR-2A 抗体 (A-4) HRP

sc-166775 HRP
200 µg/ml
RMB2377.00

SR-2A 抗体 (A-4) FITC

sc-166775 FITC
200 µg/ml
RMB2482.00

SR-2A 抗体 (A-4) PE

sc-166775 PE
200 µg/ml
RMB2580.00

SR-2A 抗体 (A-4) Alexa Fluor® 488

sc-166775 AF488
200 µg/ml
RMB2685.00

SR-2A 抗体 (A-4) Alexa Fluor® 546

sc-166775 AF546
200 µg/ml
RMB2685.00

SR-2A 抗体 (A-4) Alexa Fluor® 594

sc-166775 AF594
200 µg/ml
RMB2685.00

SR-2A 抗体 (A-4) Alexa Fluor® 647

sc-166775 AF647
200 µg/ml
RMB2685.00

SR-2A 抗体 (A-4) Alexa Fluor® 680

sc-166775 AF680
200 µg/ml
RMB2685.00

SR-2A 抗体 (A-4) Alexa Fluor® 790

sc-166775 AF790
200 µg/ml
RMB2685.00

What secondary is best to use with SR-2A (A-4): sc-166775 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with SR-2A (A-4): sc-166775 monoclonal antibody, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-19
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Rated 1 out of 5 by from Detection of false positive signalIn western blot using anti-SR-2A antibody (A-4, Cat# sc-166775) we detected protein of expected weight but in both positive (placental tissue, platelets) and negative (thymus tissue, HepG2) controls indicating false positive and unspecific detection.
Date published: 2021-12-02
Rated 1 out of 5 by from Not good for immunohistofluorescenceI did immunohistofluorescence by using a concentration 1:100 for the SR-2A antibody in the frontal cortex of a WT and KO mouse and the immunoreactivity was low, and very similar in both. Also, the signal pattern was not similar to the GFP-5HT2A mice.
Date published: 2018-03-24
Rated 5 out of 5 by from Yes, It works excellentlyin Flow cytometerInitially I was not sure about the working of the SC-166775 in human samples on flow cytometer analysis but it works upto mark,Although my experiment still needs some standarization but I am now fully satisfied with this product. Thanks Santacruz for the sample,Now I ordered full.
Date published: 2017-05-13
Rated 5 out of 5 by from Outstanding !!Strong WB,IF and IHC staining data; much better product than polyclonal alternatives
Date published: 2017-02-02
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of SR-2A expression in HT-1080, HEK293, PC-3, JAR, U-87 MG and T24 whole cell lysates.
Date published: 2017-01-11
Rated 5 out of 5 by from Good specificity in WB in HEK293 whole cell lysateGood specificity in WB in HEK293 whole cell lysate. -SCBT QC
Date published: 2015-04-11
Rated 5 out of 5 by from Beautiful immunoperoxidase cytoplasmic stainingBeautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
Date published: 2015-02-08
Rated 5 out of 5 by from Positive signal in WB in HTPositive signal in WB in HT-1080, HEK293, PC-3, JAR, U-87 MG and T24 whole cell lysates. -SCBT QC
Date published: 2015-01-08
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SR-2A 抗体 (A-4) is rated 4.0 out of 5 by 8.
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