Date published: 2025-9-7
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SPT5 Antibody (D-3) - Western Blotting - Image 386711
SPT5 抗体 (D-3): sc-133217. CCRF-CEM (A), DU 145 (B) 和 HeLa (C) 核提取物及 F9 全细胞裂解液 (D) 中 SPT5 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
SPT5 抗体 (D-3): sc-133217. CCRF-CEM (A), DU 145 (B) 和 HeLa (C) 核提取物及 F9 全细胞裂解液 (D) 中 SPT5 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

SPT5 抗体 (D-3): sc-133217

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寫評論提問

说明书
  • SPT5 抗体 D-3 是小鼠单克隆 IgG1 κ，SPT5抗体, 在18篇文献中引用，规格为200 µg/ml
  • 免疫human来源SPT5近N-terminus的61-360氨基酸
  • SPT5 抗体 (D-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 SPT5
  • 抗SPT5抗体（D-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-133217 X, 200 µg/0.1 ml)
  • 关于如何获取SPT5 (D-3): sc-133217的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 SPT5 抗体 (D-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SPT5 抗体 (D-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Suppressor of Ty抗体，包括SPT16, SPT3, SPT4, SPT5, SPT6和SPT7L

SPT5抗体（D-3）是一种IgG1 κ小鼠单克隆SPT5抗体（也称为SUPT5H抗体或SPT5H抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类的SPT5蛋白。SPT5抗体（D-3）既可以是非偶联的抗SPT5抗体形式，也可以是多种偶联形式的抗SPT5抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。SPT4（也称为Ty4和p14的抑制因子）和SPT5（也称为DSIF p160）是从酵母到人类都高度保守的蛋白质。核SPT4和SPT5既参与DRB（5,6-二氯-1-β-D-呋喃核糖基苯并咪唑）介导的转录抑制，也参与HIV-1蛋白Tat对转录延长的激活。SPT4与SPT5结合形成DSIF（DRB敏感性诱导因子）复合物，该复合物与RNA聚合酶II结合并直接调节延长。然而，由于SPT5磷酸化的增强，有丝分裂HeLa细胞中的SPT5蛋白在SDS-PAGE上的迁移速度比从间期细胞中分离出的SPT5慢。SPT5的C端CTR1结构域是P-TEFb磷酸化的底物，这对于SPT5作为转录延长调节因子的功能至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SPT5 抗体 (D-3) 参考文献:

  1. 人类转录延伸因子 DSIF 的结构和功能。  |  Yamaguchi, Y., et al. 1999. J Biol Chem. 274: 8085-92. PMID: 10075709
  2. SPT5 蛋白中调节其转录调控特性的结构域。  |  Ivanov, D., et al. 2000. Mol Cell Biol. 20: 2970-83. PMID: 10757782
  3. 剪接阵列实验分析阐明了染色质延伸因子 Spt4-5 在剪接中的作用。  |  Xiao, Y., et al. 2005. PLoS Comput Biol. 1: e39. PMID: 16172632
  4. Ssu72 C-末端结构域磷酸酶在 RNA 聚合酶 II 转录延伸中的作用  |  Reyes-Reyes, M. and Hampsey, M. 2007. Mol Cell Biol. 27: 926-36. PMID: 17101794
  5. 一种推定的转录延伸因子 hIws1 对哺乳动物细胞增殖至关重要。  |  Liu, Z., et al. 2007. Biochem Biophys Res Commun. 353: 47-53. PMID: 17184735
  6. MYC 将 SPT5 募集到 RNA 聚合酶 II，以促进转录过程的延长。  |  Baluapuri, A., et al. 2019. Mol Cell. 74: 674-687.e11. PMID: 30928206
  7. Spt5 磷酸化和 Rtf1 Plus3 域通过不同的机制促进 Rtf1 的功能  |  Chen, JJ., et al. 2020. Mol Cell Biol. 40: PMID: 32366382
  8. RBM22 通过协调 7SK-P-TEFb 复合物和 SPT5 来调节 RNA 聚合酶 II 的 5' 暂停、延伸率和终止子。  |  Du, X., et al. 2024. Genome Biol. 25: 102. PMID: 38641822
  9. 鉴定和分析酿酒酵母 SPT4 基因的功能性人类同源物。  |  Hartzog, GA., et al. 1996. Mol Cell Biol. 16: 2848-56. PMID: 8649394
  10. 酵母 SPT4 基因的人类和小鼠同源物（SUPT4H 和 Supt4h）的分离和特征。  |  Chiang, PW., et al. 1996. Genomics. 34: 368-75. PMID: 8786137
  11. DSIF是一种新型转录延伸因子，可调节RNA聚合酶II的过程性，由人类Spt4和Spt5同源物组成。  |  Wada, T., et al. 1998. Genes Dev. 12: 343-56. PMID: 9450929
  12. 有证据表明，P-TEFb 可减轻 DSIF 对 RNA 聚合酶 II 依赖性体外转录的负面影响。  |  Wada, T., et al. 1998. EMBO J. 17: 7395-403. PMID: 9857195

订购信息

产品名称产品编号规格价格数量收藏夹

SPT5 抗体 (D-3)

sc-133217
200 µg/ml
RMB2377.00

SPT5 (D-3): m-IgG Fc BP-HRP 套装

sc-528773
200 µg Ab; 10 µg BP
RMB2662.00

SPT5 (D-3): m-IgGκ BP-HRP 套装

sc-521279
200 µg Ab, 40 µg BP
RMB2662.00

SPT5 (D-3): m-IgG1 BP-HRP 套装

sc-543120
200 µg Ab; 20 µg BP
RMB2662.00

SPT5 抗体 (D-3) X

sc-133217 X
200 µg/0.1 ml
RMB2377.00

SPT5 抗体 (D-3) AC

sc-133217 AC
500 µg/ml, 25% agarose
RMB3129.00

SPT5 抗体 (D-3) HRP

sc-133217 HRP
200 µg/ml
RMB2377.00

SPT5 抗体 (D-3) FITC

sc-133217 FITC
200 µg/ml
RMB2482.00

SPT5 抗体 (D-3) PE

sc-133217 PE
200 µg/ml
RMB2580.00

SPT5 抗体 (D-3) Alexa Fluor® 488

sc-133217 AF488
200 µg/ml
RMB2685.00

SPT5 抗体 (D-3) Alexa Fluor® 546

sc-133217 AF546
200 µg/ml
RMB2685.00

SPT5 抗体 (D-3) Alexa Fluor® 594

sc-133217 AF594
200 µg/ml
RMB2685.00

SPT5 抗体 (D-3) Alexa Fluor® 647

sc-133217 AF647
200 µg/ml
RMB2685.00

SPT5 抗体 (D-3) Alexa Fluor® 680

sc-133217 AF680
200 µg/ml
RMB2685.00

SPT5 抗体 (D-3) Alexa Fluor® 790

sc-133217 AF790
200 µg/ml
RMB2685.00

Does this antibody (sc-133217) can recognize the NGN domain of SPT5?

Asked by: Anonymous
Thank you for your question . SPT5 (D-3) recognizes the N-terminus of SPT5 of human origin.
Answered by: BlakeJ
Date published: 2023-11-15

Hi, we just bought the Ab sc-133217 X for ChIP. I would like to know if you have determined the optimal amount of Ab to use for ChIP assays. Thank you.

Asked by: Noe Seija
Thank you for your question. Our general recommendation in our ChIP protocol for our antibodies is to use 0.1-1 µl TransCruz reagent (0.2–2 µg) antibody per 100–500 µg supernatant. There is also one product citation using this antibody for ChIP. Please refer to PMID: 24327761 (also shown on our website) for further details about how the antibody was used.
Answered by: Technical Support
Date published: 2020-05-07

What is the optimum concentration of sc-133217 antibody to be used in IP?

Asked by: Ashwini Challa
Thank you for your question. We recommend using 0.2-2 ug primary antibody (optimal antibody concentration should be determined by titration).
Answered by: Technical Support
Date published: 2023-11-15

When should the TransCruz reagent sc-133217 X be used instead of the standard form sc-133217?

Asked by: Professor Griffin
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good antibody for Western BlotGood antibody for western blot. Tried in HCT-116 cell lysate.
Date published: 2018-03-15
Rated 5 out of 5 by from positive stainingUse this antibody 1:200 for IHC analyisis of Mouse colon tissue, it gave the positive staining,which demstrated that protein expression in this sample.
Date published: 2017-07-08
Rated 5 out of 5 by from Clean bandClean band observed in Western Blot analysis of SPT5 expression in HeLa whole cell lysate.
Date published: 2017-05-28
Rated 5 out of 5 by from stable qualitySPT5 antibody use many times, bought two antibodies but different batches this time, the quality is stable, It got a pretty good impression of our lab.
Date published: 2017-05-28
Rated 5 out of 5 by from Good working antibody.This antibody is good in western blot analysis,got a pretty good impression of our lab.
Date published: 2017-04-29
Rated 5 out of 5 by from strong bandsNice and strong bands in detect human brain tissue with very low background.
Date published: 2017-04-15
Rated 5 out of 5 by from Antibody is the best I've ever usedAntibody shows hight express in rat spleen tissue,antibody is the best I've ever used.
Date published: 2017-02-07
Rated 5 out of 5 by from Great for IHC(P)The SPT5 Antibody (D-3)): sc-133217 provides excellent data for IHC with formalin fixed, paraffin-embedded tissue sections, showing staining pattern with nuclear and cytoplasmic localization.
Date published: 2017-02-02
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SPT5 抗体 (D-3) is rated 5.0 out of 5 by 12.
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