Date published: 2025-9-7
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Sos 2 Antibody (G-7) - Immunohistochemistry - Image 13318
Sos 2 抗体 (G-7): sc-25350. 福尔马林固定, 石蜡包埋的人胰腺组织的免疫过氧化物酶染色, 显示胰腺外分泌细胞和胰岛细胞的胞浆染色. 瑞典人类蛋白质图谱 (HPA) 计划友情提供.
Sos 2 Antibody (G-7) - Immunohistochemistry - Image 13318
Sos 2 抗体 (G-7): sc-25350. 福尔马林固定, 石蜡包埋的人胰腺组织的免疫过氧化物酶染色, 显示胰腺外分泌细胞和胰岛细胞的胞浆染色. 瑞典人类蛋白质图谱 (HPA) 计划友情提供.
Sos 2 Antibody (G-7) - Immunofluorescence - Image 4304
Sos 2 抗体 (G-7): sc-25350. 免疫荧光染色甲醇固定的 K-562 细胞, 显示细胞质定位.
Sos 2 Antibody (G-7) - Western Blotting - Image 6176
Sos 2 抗体 (G-7): sc-25350. 人重组 Sos 2 融合蛋白的 Western 印迹分析.
Sos 2 抗体 (G-7): sc-25350. 福尔马林固定, 石蜡包埋的人胰腺组织的免疫过氧化物酶染色, 显示胰腺外分泌细胞和胰岛细胞的胞浆染色. 瑞典人类蛋白质图谱 (HPA) 计划友情提供.

Sos 2 抗体 (G-7): sc-25350

3.3(3)
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说明书
  • Sos 2抗体(G-7)是小鼠单克隆IgG2b (kappa轻链)，规格为200 µg/ml
  • 免疫human物种的Sos 2的氨基酸1091-1170
  • 推荐用于 human 来源的Sos 2 WB, IP, IF, IHC(P) 和 ELISA检测
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Sos 2 抗体 (G-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Sos 2 抗体 (G-7) 组合成套装提供。 (请参阅下面的订购信息)。

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    描述
    基因信息
    蛋白序列
    说明书与实验方案
    研究信息

    関連項目

    此外 Sos 抗体 包括 Sos 2

    GTP结合蛋白的超家族，其中Ras蛋白是原型，已涉及广泛的生物活动。研究已经确定了一类鸟嘌呤核苷酸释放因子(GRFs)，它们激活哺乳动物细胞中的Ras，以及一种"适配器"蛋白(Sem 5/GRB2)，它似乎介导GRFs与活化受体分子的相互作用。Ras-GRF p140促进Ras p21s上的核苷酸交换，但不促进Ras基因超家族的其他成员上的核苷酸交换。此外，还描述了果蝇Ras-GRF的三个哺乳动物同源物，即son of sevenless (Sos)。其中包括两个来自小鼠的mSos 1和mSos 2，以及一个来自人类的hSos。据报道，Vav p95在T细胞受体激活Ras的过程中起GRF的作用，并且据报道，Vav p95具有与Dbl p115相似的结构域，Dbl p115是Cdc42Hs特异的GRF。激活后，Ras似乎与Raf相互作用，从而激活MAP激酶磷酸化途径。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Sos 2 抗体 (G-7) 参考文献:

    1. SOS2 和 LZTR1 的罕见变异与努南综合征有关。  |  Yamamoto, GL., et al. 2015. J Med Genet. 52: 413-21. PMID: 25795793
    2. 影响 SOS2 Dbl 同源域的激活突变会导致努南综合征。  |  Cordeddu, V., et al. 2015. Hum Mutat. 36: 1080-7. PMID: 26173643
    3. 致癌的 RAS 异构体显示出对鸟嘌呤核苷酸交换因子 SOS2 的分级需求，以介导细胞转化体。  |  Sheffels, E., et al. 2018. Sci Signal. 11: PMID: 30181243
    4. 锚定依赖性生长条件揭示了 RAS 突变癌细胞的转化和存活对 SOS2 的不同依赖性。  |  Sheffels, E., et al. 2021. Small GTPases. 12: 67-78. PMID: 31062644
    5. SOS2 变异是导致努南综合征的一个罕见病因，特别容易引起淋巴并发症。  |  Lissewski, C., et al. 2021. Eur J Hum Genet. 29: 51-60. PMID: 32788663
    6. SOS2 脱颖而出：生理学和病理学中的不同功能。  |  Baltanás, FC., et al. 2021. Int J Mol Sci. 22: PMID: 34205562
    7. RAS通路基因中SOS2、MAP2K1和RASGRF2的遗传变异可预测HBV相关肝细胞癌患者的生存率。  |  Lin, Q., et al. 2023. Arch Toxicol. 97: 1599-1611. PMID: 37029817
    8. SOS2调节表皮生长因子受体信号转导的阈值，从而调控奥希替尼在肺腺癌中的疗效和耐药性。  |  Theard, PL., et al. 2024. Mol Oncol. 18: 641-661. PMID: 38073064
    9. 发现与 '无七之子 2'（SOS2）结合的小分子。  |  Zak, KM., et al. 2025. J Med Chem. 68: 2680-2693. PMID: 39818983
    10. 人类 Sos1 和 Sos2 与 Grb2 的不同相互作用。  |  Yang, SS., et al. 1995. J Biol Chem. 270: 18212-5. PMID: 7629138

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Sos 2 抗体 (G-7)

    sc-25350
    200 µg/ml
    		RMB2377.00

    Sos 2 (G-7): m-IgG Fc BP-HRP 套装

    sc-536707
    200 µg Ab; 10 µg BP
    		RMB2662.00

    Sos 2 (G-7): m-IgGκ BP-HRP 套装

    sc-533867
    200 µg Ab; 40 µg BP
    		RMB2662.00

    Sos 2 (G-7): m-IgG2b BP-HRP 套装

    sc-549821
    200 µg Ab; 10 µg BP
    		RMB2662.00

    Can Sos 2 (G-7): sc-25350 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: DefinitelyNotMatt
    Thank you for your question. Yes, Sos 2 (G-7): sc-25350 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 1 out of 5 by from anti-SOS2 clone G-7 did not work in a WesternI've been struggling to see a signal for SOS2 in Westerns of HeLa lysates. A coworker tested several antibodies from other vendors and chose one that just wasn't very potent. I tested 3 SCBT antibodies (clones A-8, B-6, and G-7) in a single experiment: HeLa cells vs LN229 cells, treated with an inhibitor or DMSO control and then stimulated with EGF for 5'; 40µg whole cell lysate (using NP40) per lane on a 3%-8% tris-acetate gel run at 85V for 1.2hrs, then turned up to 135V till the dye ran off the bottom. Protein transfer was to a single PVDF membrane in an iBlot2 with a custom program (created to transfer large proteins). After transfer, the blot was cut into 3 blocks. All blocks were blocked with 5% BSA in TBS for 1.5hrs, then Tween-20 was added to 0.1%, primary antibody was added at a 1:400 dilution, and the blots were incubated overnight in a cold room on a rocker. The following day, the blots were washed 4 times (5' each) with TBST, then incubated with Licor IR800 secondary antibody. Incubation was for 1.5hrs after which they were washed 4 times (5' each) and imaged using a Licor Odyssey. Only the B6 antibody showed banding. Clone G-7 produced no banding at all as seen in the attached image.
    Date published: 2020-01-03
    Rated 4 out of 5 by from Band of interest seen in WB using human recombinantBand of interest seen in WB using human recombinant Sos 2 fusion protein. -SCBT QC
    Date published: 2015-06-09
    Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmicProduced superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
    Date published: 2015-05-21
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    Sos 2 抗体 (G-7) is rated 3.3 out of 5 by 3.
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