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Sos 2 Antibody (A-8) - Western Blotting - Image 13647
Sos 2 抗体 (A-8): sc-55478. 人重组 Sos 2 融合蛋白的 Western 印迹分析.
Sos 2 Antibody (A-8) - Western Blotting - Image 13647
Sos 2 抗体 (A-8): sc-55478. 人重组 Sos 2 融合蛋白的 Western 印迹分析.
Sos 2 抗体 (A-8): sc-55478. 人重组 Sos 2 融合蛋白的 Western 印迹分析.

Sos 2 抗体 (A-8): sc-55478

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说明书
  • Sos 2抗体(A-8)是小鼠单克隆IgG2a (kappa轻链)，规格为200 µg/ml
  • 免疫human来源Sos 2近C-terminus的1091-1170氨基酸
  • 推荐用于 human 来源的Sos 2 WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRP是Sos 2 Antibody (A-8) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Sos 2 Antibody (A-8) 搭配使用（请参阅下面的订购信息）。

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    描述
    基因信息
    蛋白序列
    说明书与实验方案
    研究信息

    関連項目

    额外Sos抗体，包括Sos 2

    GTP结合蛋白的超家族，其中Ras蛋白是原型，已涉及广泛的生物活动。研究已经确定了一类鸟嘌呤核苷酸释放因子(GRFs)，它们激活哺乳动物细胞中的Ras，以及一种"适配器"蛋白(Sem 5/GRB2)，它似乎介导GRFs与活化受体分子的相互作用。Ras-GRF p140促进Ras p21s上的核苷酸交换，但不促进Ras基因超家族的其他成员上的核苷酸交换。此外，还描述了果蝇Ras-GRF的三个哺乳动物同源物，即son of sevenless (Sos)。其中包括两个来自小鼠的mSos 1和mSos 2，以及一个来自人类的hSos。据报道，Vav p95在T细胞受体激活Ras的过程中起GRF的作用，并且据报道，Vav p95具有与Dbl p115相似的结构域，Dbl p115是Cdc42Hs特异的GRF。激活后，Ras似乎与Raf相互作用，从而激活MAP激酶磷酸化途径。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Sos 2 抗体 (A-8) 参考文献:

    1. 胰岛素信号基因与阿尔茨海默病的候选基因关联研究：SOS2、PCK1 和 PPARgamma 作为易感基因位点的证据。  |  Hamilton, G., et al. 2007. Am J Med Genet B Neuropsychiatr Genet. 144B: 508-16. PMID: 17440948
    2. 土拉弗氏菌通过将 PKCα 和 βI 招募到 SOS2/GrB2 复合物来触发细胞内的 Ras 信号，这对细菌在细胞质中的增殖至关重要。  |  Al-Khodor, S. and Abu Kwaik, Y. 2010. Cell Microbiol. 12: 1604-21. PMID: 20618341
    3. SOS2 和 LZTR1 的罕见变异与努南综合征有关。  |  Yamamoto, GL., et al. 2015. J Med Genet. 52: 413-21. PMID: 25795793
    4. 通过大规模外显子组芯片分析发现的 SOS2 和 ACP1 基因座调控肾脏的发育和功能  |  Li, M., et al. 2017. J Am Soc Nephrol. 28: 981-994. PMID: 27920155
    5. SOS 全球环境基金在健康和疾病方面的作用。  |  Baltanás, FC., et al. 2020. Biochim Biophys Acta Rev Cancer. 1874: 188445. PMID: 33035641
    6. 首例 SOS2 基因突变的努南综合征产前病例：早期诊断对遗传咨询的意义。  |  Gentile, M., et al. 2021. Am J Med Genet A. 185: 1897-1902. PMID: 33750022
    7. SOS2 脱颖而出：生理学和病理学中的不同功能。  |  Baltanás, FC., et al. 2021. Int J Mol Sci. 22: PMID: 34205562
    8. 合成维甲酸磺达罗汀通过抑制 SOS2-RAS 通路克服肝细胞癌的干性和索拉非尼耐药性。  |  Qi, F., et al. 2021. J Exp Clin Cancer Res. 40: 280. PMID: 34479623
    9. 努南综合征患者一生中的淋巴异常：回顾性队列研究  |  Swarts, JW., et al. 2022. Am J Med Genet A. 188: 3242-3261. PMID: 35979676
    10. 编码人类ras交换因子的两个基因的染色体定位：SOS1 映射到人类基因组的 2p22-->p16 区域，SOS2 映射到 14q21-->q22 区域。  |  Chardin, P. and Mattei, MG. 1994. Cytogenet Cell Genet. 66: 68-9. PMID: 8275713

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Sos 2 抗体 (A-8)

    sc-55478
    200 µg/ml
    		RMB2377.00

    Sos 2 (A-8): m-IgG Fc BP-HRP 套装

    sc-539020
    200 µg Ab; 10 µg BP
    		RMB2662.00

    How much sample do you recommend loading on the gel for Western blot detection using Sos 2 (A-8): sc-55478?

    Asked by: Cweed
    We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-01
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    • QUESTIONS, PRODUCT
    Rated 1 out of 5 by from Clone A-8 did not work for me at all in a Westernclone A-8 did NOT work in a Western using HeLa and LN229 I've been struggling to see a signal for SOS2 in HeLa lysates. A coworker tested several antibodies from other vendors and chose one that just wasn't very potent. I tested 3 SCBT antibodies (clones A-8, B-6, and G-7) in a single experiment: HeLa cells vs LN229 cells, treated with an inhibitor or DMSO control and then stimulated with EGF for 5'; 40µg whole cell lysate (using NP40) per lane on a 3%-8% tris-acetate gel run at 85V for 1.2hrs, then turned up to 135V till the dye ran off the bottom. Protein transfer was to a single PVDF membrane in an iBlot2 with a custom program (created to transfer large proteins). After transfer, the blot was cut into 3 blocks. All blocks were blocked with 5% BSA in TBS for 1.5hrs, then Tween-20 was added to 0.1%, primary antibody was added at a 1:400 dilution, and the blots were incubated overnight in a cold room on a rocker. The following day, the blots were washed 4 times (5' each) with TBST, then incubated with Licor IR800 secondary antibody. Incubation was for 1.5hrs after which they were washed 4 times (5' each) and imaged using a Licor Odyssey. Only the B6 antibody showed banding. Clone A-8 produced no banding at all as seen in the attached image.
    Date published: 2020-01-03
    Rated 5 out of 5 by from Good Western blot data of human recombinantGood Western blot data of human recombinant Sos 2 fusion protein. -SCBT QC
    Date published: 2013-07-07
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    Sos 2 抗体 (A-8) is rated 3.0 out of 5 by 2.
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