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Superoxide Dismutase 2/SOD2 Antibody (A-2) - Western Blotting - Image 380259
SOD-2 抗体 (A-2): sc-133134. SH-SY5Y (A), T98G (B), A-673 (C), Sol8 (D) 和 A-10 (E) 全细胞裂解液中 SOD-2 表达的 Western 印迹分析.
SOD-2 抗体 (A-2): sc-133134. SH-SY5Y (A), T98G (B), A-673 (C), Sol8 (D) 和 A-10 (E) 全细胞裂解液中 SOD-2 表达的 Western 印迹分析.

Superoxide Dismutase 2/SOD2 抗体 (A-2): sc-133134

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寫評論提問

说明书
  • Superoxide Dismutase 2/SOD2抗体(A-2)是小鼠单克隆IgG2b κ, 在100篇文献中引用，规格为200 µg/ml
  • 免疫human物种的代表全长SOD-2的氨基酸1-222
  • 抗-Superoxide Dismutase 2/SOD2 抗体 (A-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 SOD-2
  • 抗Superoxide Dismutase 2/SOD2抗体（A-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Superoxide Dismutase 2/SOD2 (A-2): sc-133134的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Superoxide Dismutase 2/SOD2 抗体 (A-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Superoxide Dismutase 2/SOD2 抗体 (A-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加SOD抗体，包括SOD-1, SOD-2和SOD-3

超氧化物歧化酶2/SOD2抗体（A-2）是一种IgG2b κ型小鼠单克隆SOD-2抗体（也称为SOD-2抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的SOD-2。超氧化物歧化酶2/SOD2抗体（A-2）既可以是非偶联的抗SOD-2抗体形式，也可以是多种偶联形式的抗SOD-2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。超氧化物歧化酶家族由三种金属酶（SOD-1、SOD-2和SOD-3）组成，可催化活性氧（ROS）如超氧阴离子的氧化还原。SOD-2前体是一种由核染色质编码的222个氨基酸的蛋白质，在细胞质中合成并在翻译后导入线粒体基质。与作为同源二聚体细胞质Cu-Zn酶的SOD-1不同，SOD-2是一种同源四聚体锰酶（也称为MnSOD），在线粒体中发挥作用。ROS涉及广泛的退行性过程，包括阿尔茨海默病、帕金森病和缺血性心脏病。缺乏SOD-2的纯合突变小鼠表现出扩张型心肌病、肝脏和骨骼肌中脂质积聚、代谢性酸中毒、氧化性DNA损伤以及心脏和骨骼肌中的呼吸链缺陷。SOD-2基因的多态性也与人类的非家族性、特发性扩张型心肌病有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Superoxide Dismutase 2/SOD2 抗体 (A-2) 参考文献:

  1. 日本人的 SOD2 和 HLA-DRB1 基因多态性与非家族性特发性扩张型心肌病有关。  |  Hiroi, S., et al. 1999. Biochem Biophys Res Commun. 261: 332-9. PMID: 10425186
  2. 人类锰超氧化物歧化酶前体的合成和加工。  |  Wispé, JR., et al. 1989. Biochim Biophys Acta. 994: 30-6. PMID: 2462451
  3. 缺乏锰超氧化物歧化酶的突变小鼠会出现心肌扩张和新生儿死亡。  |  Li, Y., et al. 1995. Nat Genet. 11: 376-81. PMID: 7493016
  4. 日本扩张型心肌病患者的 HLA II 类基因 DNA 分型。  |  Nishi, H., et al. 1995. J Mol Cell Cardiol. 27: 2385-92. PMID: 8576952
  5. 人类线粒体锰超氧化物歧化酶多态变体 Ile58Thr 通过破坏四聚体界面的稳定性来降低活性。  |  Borgstahl, GE., et al. 1996. Biochemistry. 35: 4287-97. PMID: 8605177
  6. 线粒体锰超氧化物歧化酶缺乏小鼠的新型神经系统表型。  |  Melov, S., et al. 1998. Nat Genet. 18: 159-63. PMID: 9462746
  7. 探究人锰超氧化物歧化酶的活性位点：谷氨酰胺的作用 143.  |  Hsieh, Y., et al. 1998. Biochemistry. 37: 4731-9. PMID: 9537988
  8. 超氧化物歧化酶 2 突变小鼠的线粒体疾病  |  Melov, S., et al. 1999. Proc Natl Acad Sci U S A. 96: 846-51. PMID: 9927656

订购信息

产品名称产品编号规格价格数量收藏夹

Superoxide Dismutase 2/SOD2 抗体 (A-2)

sc-133134
200 µg/ml
RMB2377.00

Superoxide Dismutase 2/SOD2 (A-2): m-IgGκ BP-HRP 套装

sc-521245
200 µg Ab, 40 µg BP
RMB2662.00

Superoxide Dismutase 2/SOD2 (A-2): m-IgG2b BP-HRP 套装

sc-548878
200 µg Ab; 10 µg BP
RMB2662.00

Superoxide Dismutase 2/SOD2 抗体 (A-2) AC

sc-133134 AC
500 µg/ml, 25% agarose
RMB3129.00

Superoxide Dismutase 2/SOD2 抗体 (A-2) HRP

sc-133134 HRP
200 µg/ml
RMB2377.00

Superoxide Dismutase 2/SOD2 抗体 (A-2) FITC

sc-133134 FITC
200 µg/ml
RMB2482.00

Superoxide Dismutase 2/SOD2 抗体 (A-2) PE

sc-133134 PE
200 µg/ml
RMB2580.00

Superoxide Dismutase 2/SOD2 抗体 (A-2) Alexa Fluor® 488

sc-133134 AF488
200 µg/ml
RMB2685.00

Superoxide Dismutase 2/SOD2 抗体 (A-2) Alexa Fluor® 546

sc-133134 AF546
200 µg/ml
RMB2685.00

Superoxide Dismutase 2/SOD2 抗体 (A-2) Alexa Fluor® 594

sc-133134 AF594
200 µg/ml
RMB2685.00

Superoxide Dismutase 2/SOD2 抗体 (A-2) Alexa Fluor® 647

sc-133134 AF647
200 µg/ml
RMB2685.00

Superoxide Dismutase 2/SOD2 抗体 (A-2) Alexa Fluor® 680

sc-133134 AF680
200 µg/ml
RMB2685.00

Superoxide Dismutase 2/SOD2 抗体 (A-2) Alexa Fluor® 790

sc-133134 AF790
200 µg/ml
RMB2685.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with SOD-2 Antibody (A-2): sc-133134?

Asked by: Trav11
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-26
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Rated 5 out of 5 by from SOD2 western blot with sc-133134Very good signal in rat placental tissue. Used a 1:1000 dilution with sc-2005 as secondary antibody.
Date published: 2021-08-12
Rated 5 out of 5 by from Excellent for WBI used SOD-2 antibody for WB of cerebral cortex and hippocampal lysates of CDKL5 WT and KO mice (30ug). I used 1:500 and 1:1000 dilutions and they both worked very well.
Date published: 2020-06-08
Rated 5 out of 5 by from Works great!Works great for western at 1:500 dilution in an immortalized oral keratinocyte cell line.
Date published: 2018-05-11
Rated 4 out of 5 by from Used this for mitochondrial localizationIs a well characterized antibody for mitochondrial matrix localization and does not recognize SOD1
Date published: 2018-04-12
Rated 5 out of 5 by from wonderful primary antibody1:200 diluted for wb of rat protein,the band is strong and special
Date published: 2018-04-11
Rated 5 out of 5 by from ExcellentThis antibody gave beautiful and strong bands for western blot
Date published: 2018-03-13
Rated 5 out of 5 by from Works well in WBUsed at 1:5000 dilution on samples from rat heart and arteries transferred onto nitrocellulose membranes and probed with LiCor secondary antibodies. got a very strong, clean single band at about 23 kD.
Date published: 2017-12-05
Rated 5 out of 5 by from excellent antibody for WBIt works very well for WB. Strong signal and clear blot. I have used this antibody with 1:1000 dilution in 2% BSA for lung cancer cell line.
Date published: 2017-08-16
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Superoxide Dismutase 2/SOD2 抗体 (A-2) is rated 4.9 out of 5 by 17.
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