Date published: 2025-9-8
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SNX9 Antibody (G-5) - Western Blotting - Image 363957
SNX9 抗体 (G-5): sc-166863. HeLa (A), NIH/3T3 (B), RAW 264. 7 (C) 和 3T3-L1 (D) 全细胞裂解液中 SNX9 表达的 Western 印迹分析.
SNX9 抗体 (G-5): sc-166863. HeLa (A), NIH/3T3 (B), RAW 264. 7 (C) 和 3T3-L1 (D) 全细胞裂解液中 SNX9 表达的 Western 印迹分析.

SNX9 抗体 (G-5): sc-166863

4.3(6)
寫評論提問

说明书
  • SNX9 抗体 G-5 是小鼠单克隆 IgG1 κ，SNX9抗体, 在2篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的SNX9的内部区域内的氨基酸391-436之间
  • SNX9 抗体 (G-5) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 SNX9
  • 抗SNX9抗体（G-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SNX9 (G-5): sc-166863的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是SNX9 Antibody (G-5) for WB applications. 的首选辅助检测试剂，这些试剂现与SNX9 Antibody (G-5) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外SNX抗体，包括SNX1, SNX10, SNX11, SNX12, SNX15, SNX16, SNX17, SNX18, SNX19, SNX2, SNX20, SNX27, SNX29, SNX3, SNX4和SNX5

SNX9抗体（G-5）是一种IgG1 κ小鼠单克隆SNX9抗体（也称为分拣连蛋白-9抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的SNX9蛋白。SNX9抗体（G-5）既可以是非偶联的抗SNX9抗体形式，也可以是多种偶联形式的抗SNX9抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。分拣连蛋白（SNX）是包含亲水性PX（磷脂结合基序）结构域的一大类蛋白质的成员，它们与多种受体类型相互作用。SNX在生物体内广泛表达，尽管每种SNX mRNA的组织分布各不相同。SNX具有与特定磷脂结合的能力，以及形成蛋白质-蛋白质复合物的倾向，这表明这些蛋白质在细胞膜运输和蛋白质分拣中发挥作用。SNX还可能特异性地发挥促降解分拣、内化、内体再循环或简单的内体分拣功能。尽管SNX具有亲水性，但它们仍与细胞膜有部分关联。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SNX9 抗体 (G-5) 参考文献:

  1. 理清分拣神经蛋白的细胞功能。  |  Worby, CA. and Dixon, JE. 2002. Nat Rev Mol Cell Biol. 3: 919-31. PMID: 12461558
  2. 胰岛素刺激分拣蛋白 9 在细胞间的移动：可能是细胞表面受体表达和胰岛素作用的媒介。  |  MaCaulay, SL., et al. 2003. Biochem J. 376: 123-34. PMID: 12917015
  3. 由分拣蛋白 9 介导的动态连接蛋白-2 的膜招募调节。  |  Lundmark, R. and Carlsson, SR. 2004. J Biol Chem. 279: 42694-702. PMID: 15299020
  4. 两步差异表达分析揭示了一组参与甲状腺肿瘤的新基因。  |  Jacques, C., et al. 2005. J Clin Endocrinol Metab. 90: 2314-20. PMID: 15623817
  5. 分类nexins--统一趋势和新视角。  |  Carlton, J., et al. 2005. Traffic. 6: 75-82. PMID: 15634208
  6. SNX9 可调控动态蛋白的组装，是高效凝集素介导的内吞所必需的。  |  Soulet, F., et al. 2005. Mol Biol Cell. 16: 2058-67. PMID: 15703209
  7. SNX9 作为连接突触素-1 与 Cdc42 效应器 ACK1 的适配器。  |  Yeow-Fong, L., et al. 2005. FEBS Lett. 579: 5040-8. PMID: 16137687
  8. 分类新系统。  |  Carlton, JG. and Cullen, PJ. 2005. Curr Biol. 15: R819-20. PMID: 16243015
  9. Dynamin, 内吞和细胞内信号传导（综述）。  |  McClure, SJ. and Robinson, PJ. 1996. Mol Membr Biol. 13: 189-215. PMID: 9116759

订购信息

产品名称产品编号规格价格数量收藏夹

SNX9 抗体 (G-5)

sc-166863
200 µg/ml
RMB2377.00

SNX9 (G-5): m-IgG Fc BP-HRP 套装

sc-527192
200 µg Ab; 10 µg BP
RMB2662.00

SNX9 (G-5): m-IgG1 BP-HRP 套装

sc-532565
200 µg Ab; 20 µg BP
RMB2662.00

SNX9 抗体 (G-5) AC

sc-166863 AC
500 µg/ml, 25% agarose
RMB3129.00

SNX9 抗体 (G-5) HRP

sc-166863 HRP
200 µg/ml
RMB2377.00

SNX9 抗体 (G-5) FITC

sc-166863 FITC
200 µg/ml
RMB2482.00

SNX9 抗体 (G-5) PE

sc-166863 PE
200 µg/ml
RMB2580.00

SNX9 抗体 (G-5) Alexa Fluor® 488

sc-166863 AF488
200 µg/ml
RMB2685.00

SNX9 抗体 (G-5) Alexa Fluor® 546

sc-166863 AF546
200 µg/ml
RMB2685.00

SNX9 抗体 (G-5) Alexa Fluor® 594

sc-166863 AF594
200 µg/ml
RMB2685.00

SNX9 抗体 (G-5) Alexa Fluor® 647

sc-166863 AF647
200 µg/ml
RMB2685.00

SNX9 抗体 (G-5) Alexa Fluor® 680

sc-166863 AF680
200 µg/ml
RMB2685.00

SNX9 抗体 (G-5) Alexa Fluor® 790

sc-166863 AF790
200 µg/ml
RMB2685.00

What is the recommended starting dilution for Western blot for sc-166863: SNX9 (G-5) monoclonal antibody?

Asked by: jenniferc
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 1 out of 5 by from No bands in WBI did a 1:200 dilution of antibody with milk 5% in TBS-T. I left it overnight incubation, and when revealing, no bands appeared in the membrane.
Date published: 2018-02-15
Rated 5 out of 5 by from Good featuresWe have test it by IF in HeLa cells, we got a dots like structure in cell and no staining for the nuclei of cells which is consistent with our transfected data.
Date published: 2017-09-25
Rated 5 out of 5 by from Very gooodI used sample vial I requested from SCBT. I tried endogenous SNX9 expression of 293T cell with different plasmids of interests.
Date published: 2017-07-11
Rated 5 out of 5 by from Good detection of mouse and human SNX9This antibody works great in Western Blot, showing a single band of good strength at a consistent weight with both mouse and human cell lines.
Date published: 2017-02-03
Rated 5 out of 5 by from Worked well for Western with human SNX9 transfectedWorked well for Western with human SNX9 transfected lysate. -SCBT QC
Date published: 2015-06-13
Rated 5 out of 5 by from Publishable data with monkey BSC1Publishable data with monkey BSC1 - SCBT Publication Review
Date published: 2013-01-05
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SNX9 抗体 (G-5) is rated 4.3 out of 5 by 6.
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