Date published: 2025-9-8

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SNX27 抗体 (F-2): sc-515707

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说明书
  • SNX27 抗体 F-2 是小鼠单克隆 IgG2a κ,SNX27抗体, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的SNX27的C-terminus附近的氨基酸449-466之间
  • SNX27 抗体 (F-2) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 SNX27
  • 抗SNX27抗体(F-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SNX27 (F-2): sc-515707免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成SNX27 抗体 (F-2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

SNX27抗体(F-2)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)等方法检测小鼠、大鼠和人类样本中的SNX27。SNX27 (F-2) 抗体有非结合型和多种结合型可供选择,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。SNX27由541个氨基酸组成,在细胞内运输和回收途径中起着至关重要的作用,主要定位于细胞质和早期内体中。SNX27具有一个典型的phox同源(PX)结构域、一个Ras结合结构域和一个PDZ结构域,这些结构域对于囊泡定位和功能至关重要。SNX27与CYTIP和SR-4等蛋白相互作用,促进各种受体的内吞转运,这对于维持细胞内稳态和信号传导至关重要。SNX27有四种命名同工型,它们通过可变剪接产生,突出了在不同细胞环境中的复杂性和调控潜力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SNX27 抗体 (F-2) 参考文献:

  1. 新分选蛋白(SNX27)和 NHERF 与 5-HT4a 受体剪接变体特异性相互作用:在受体靶向中的作用。  |  Joubert, L., et al. 2004. J Cell Sci. 117: 5367-79. PMID: 15466885
  2. 分类nexins--统一趋势和新视角。  |  Carlton, J., et al. 2005. Traffic. 6: 75-82. PMID: 15634208
  3. Phox(PX)结构域蛋白与膜交通。  |  Seet, LF. and Hong, W. 2006. Biochim Biophys Acta. 1761: 878-96. PMID: 16782399
  4. 蛋白质组学鉴定分拣 nexin 27 为二酰基甘油激酶 zeta 相关蛋白:二酰基甘油激酶在内循环中的新作用。  |  Rincón, E., et al. 2007. Mol Cell Proteomics. 6: 1073-87. PMID: 17351151
  5. 分拣蛋白 27 与淋巴细胞中的细胞粘合素相关支架蛋白(CASP)相互作用。  |  MacNeil, AJ., et al. 2007. Biochem Biophys Res Commun. 359: 848-53. PMID: 17577583
  6. 迁移和肿瘤激活的自然杀伤细胞中内含体 SNX27 的极化。  |  MacNeil, AJ. and Pohajdak, B. 2007. Biochem Biophys Res Commun. 361: 146-50. PMID: 17644068
  7. 一种独特的分拣蛋白通过 PDZ 结构域相互作用调节钾通道的迁移。  |  Lunn, ML., et al. 2007. Nat Neurosci. 10: 1249-59. PMID: 17828261
  8. Kir3 通道亚基特异性地受分拣 nexin 27 的调控。  |  Nassirpour, R. and Slesinger, PA. 2007. Channels (Austin). 1: 331-3. PMID: 18690037
  9. 背角神经元中 VPS26A-SNX27 相互作用依赖的 mGluR5 循环介导大鼠的神经性疼痛  |  Lin, TB., et al. 2015. J Neurosci. 35: 14943-55. PMID: 26538661
  10. SNX27缺失通过损害脑外膜细胞分化和纤毛生成导致脑积水  |  Wang, X., et al. 2016. J Neurosci. 36: 12586-12597. PMID: 27974614
  11. SNX27-FERM-SNX1复合物结构合理化了SNX17和SNX27的不同贩运途径  |  Yong, X., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34462354
  12. SNX27-Retromer在囊泡回收过程中直接结合ESCPE-1以转移货物蛋白。  |  Simonetti, B., et al. 2022. PLoS Biol. 20: e3001601. PMID: 35417450

订购信息

产品名称产品编号规格价格数量收藏夹

SNX27 抗体 (F-2)

sc-515707
200 µg/ml
RMB2377.00

SNX27 抗体 (F-2) AC

sc-515707 AC
500 µg/ml, 25% agarose
RMB3129.00

SNX27 抗体 (F-2) HRP

sc-515707 HRP
200 µg/ml
RMB2377.00

SNX27 抗体 (F-2) FITC

sc-515707 FITC
200 µg/ml
RMB2482.00

SNX27 抗体 (F-2) PE

sc-515707 PE
200 µg/ml
RMB2580.00

SNX27 抗体 (F-2) Alexa Fluor® 488

sc-515707 AF488
200 µg/ml
RMB2685.00

SNX27 抗体 (F-2) Alexa Fluor® 546

sc-515707 AF546
200 µg/ml
RMB2685.00

SNX27 抗体 (F-2) Alexa Fluor® 594

sc-515707 AF594
200 µg/ml
RMB2685.00

SNX27 抗体 (F-2) Alexa Fluor® 647

sc-515707 AF647
200 µg/ml
RMB2685.00

SNX27 抗体 (F-2) Alexa Fluor® 680

sc-515707 AF680
200 µg/ml
RMB2685.00

SNX27 抗体 (F-2) Alexa Fluor® 790

sc-515707 AF790
200 µg/ml
RMB2685.00

How well does the Anti-SNX27 Antibody (F-2): sc-515707 work for IHC(P) as there is no information for it on the datasheet?

Asked by: Hann
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-01-12

What is the recommended starting dilution for Western blot for SNX27 (F-2): sc-515707 monoclonal antibody?

Asked by: TinTin
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-515707.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-02-27
  • y_2025, m_9, d_7, h_4CST
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Rated 5 out of 5 by from Good for Western BlotAntibody works consistently in 3T3-L1 whole cell lysate. Shown with Cruz Marker molecular weight standards, sc-2035. -SCBT QC
Date published: 2023-09-14
Rated 1 out of 5 by from Didn't work for meI used this AB for WesternBlots with MCF7 whole cell lysate Poor signal detected by ECL, several bands, nothing specific. Not better with high sensitivity detection reagent : more bands but nothing specific.
Date published: 2020-07-31
Rated 5 out of 5 by from NiceStrong bands in WB with NIH/3T3 and 3T3-L1 whole cell lysates,clean,nice.
Date published: 2017-01-05
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SNX27 抗体 (F-2) is rated 3.7 out of 5 by 3.
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