Date published: 2025-9-8

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Snk 抗体 (E-10): sc-374643

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说明书
  • Snk 抗体 E-10 是小鼠单克隆 IgG1 κ,Snk抗体, 在4篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Snk的N-terminus的氨基酸2-27之间
  • Snk 抗体 (E-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Snk; 也和以下物种反应,包括: 和 bovine and porcine
  • 抗Snk抗体(E-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Snk (E-10): sc-374643免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 Snk 抗体 (E-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Snk 抗体 (E-10) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Snk抗体(E-10)是一种IgG1 κ小鼠单克隆Snk抗体(也称为Polo样激酶2抗体、血清诱导激酶抗体、SNK抗体、丝氨酸/苏氨酸蛋白激酶PLK2抗体、丝氨酸/苏氨酸蛋白激酶SNK抗体、HPlk2抗体或PLK-2抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Snk蛋白。Snk抗体(E-10)既可以作为非偶联的抗Snk抗体形式存在,也可以作为多种偶联形式的抗Snk抗体存在,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Plks(类Polo激酶)编码丝氨酸/苏氨酸激酶,它们与Polo和CDC5密切相关,这些基因分别是果蝇和酵母通过有丝分裂所必需的。类Polo激酶,包括Plk、Snk(针对血清诱导激酶,也称为Plk2)和Fnk(针对FGF诱导激酶,也称为Plk3或PRK),在细胞增殖中发挥作用。Plk蛋白在细胞周期的S期和G2期在细胞中积累,蛋白含量和催化活性在有丝分裂开始时达到峰值,随后在有丝分裂后迅速降低。Snk和Fnk是即早反应基因,在G1期首先表达。Fnk的表达在S期末和G2期达到峰值,它可能在调节M期的开始中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Snk 抗体 (E-10) 参考文献:

  1. [Fidarestat (SNK-860)]。  |  Hibi, C. 2002. Nihon Rinsho. 60 Suppl 9: 594-600. PMID: 12387056
  2. Polo样激酶2(SNK/PLK2)的转录物沉默是B细胞恶性肿瘤中常见的情况。  |  Syed, N., et al. 2006. Blood. 107: 250-6. PMID: 16160013
  3. 体外处理淀粉样蛋白-β肽的海马神经元中 SNK 和 SPAR 信号通路的变化。  |  Sui, H., et al. 2017. Neuropeptides. 63: 43-48. PMID: 28400058
  4. 微波辐射通过 SNK-SPAR 通路导致海马神经元树突棘收缩  |  Zhi, WJ., et al. 2018. Brain Res. 1679: 134-143. PMID: 29180226
  5. polo是果蝇的一种有丝分裂突变体,显示出异常的纺锤极。  |  Sunkel, CE. and Glover, DM. 1988. J Cell Sci. 89 (Pt 1): 25-38. PMID: 3417791
  6. 急性MPTP治疗通过C57BL/6小鼠中的SNK-SPAR通路降低纹状体中刺突神经元的树突棘密度。  |  Su, Y., et al. 2022. Synapse. 76: e22249. PMID: 36008099
  7. 编码一种保守的有丝分裂蛋白激酶的小鼠 mRNA 的细胞周期和末期分化缔合作用调控。  |  Lake, RJ. and Jelinek, WR. 1993. Mol Cell Biol. 13: 7793-801. PMID: 7902533
  8. 果蝇 polo 丝氨酸-苏氨酸激酶的人类和鼠类同源物的克隆与表征。  |  Hamanaka, R., et al. 1994. Cell Growth Differ. 5: 249-57. PMID: 8018557
  9. 酿酒酵母 G1 细胞周期突变基因 dbf4 的一个多拷贝抑制基因编码一种蛋白激酶,被鉴定为 CDC5。  |  Kitada, K., et al. 1993. Mol Cell Biol. 13: 4445-57. PMID: 8321244
  10. Prk是一种细胞因子诱导的人类蛋白丝氨酸/苏氨酸激酶,在肺癌中的表达似乎下调。  |  Li, B., et al. 1996. J Biol Chem. 271: 19402-8. PMID: 8702627
  11. 成纤维细胞生长因子诱导激酶(Fnk)在细胞周期进展过程中的表达和磷酸化。  |  Chase, D., et al. 1998. Biochem J. 333 (Pt 3): 655-60. PMID: 9677325
  12. Polo-like 激酶:整个有丝分裂过程中都在发挥作用的团队。  |  Glover, DM., et al. 1998. Genes Dev. 12: 3777-87. PMID: 9869630

订购信息

产品名称产品编号规格价格数量收藏夹

Snk 抗体 (E-10)

sc-374643
200 µg/ml
RMB2377.00

Snk (E-10): m-IgG Fc BP-HRP 套装

sc-527496
200 µg Ab; 10 µg BP
RMB2662.00

Snk (E-10): m-IgG1 BP-HRP 套装

sc-532869
200 µg Ab; 20 µg BP
RMB2662.00

Snk 抗体 (E-10) AC

sc-374643 AC
500 µg/ml, 25% agarose
RMB3129.00

Snk 抗体 (E-10) HRP

sc-374643 HRP
200 µg/ml
RMB2377.00

Snk 抗体 (E-10) FITC

sc-374643 FITC
200 µg/ml
RMB2482.00

Snk 抗体 (E-10) PE

sc-374643 PE
200 µg/ml
RMB2580.00

Snk 抗体 (E-10) Alexa Fluor® 488

sc-374643 AF488
200 µg/ml
RMB2685.00

Snk 抗体 (E-10) Alexa Fluor® 546

sc-374643 AF546
200 µg/ml
RMB2685.00

Snk 抗体 (E-10) Alexa Fluor® 594

sc-374643 AF594
200 µg/ml
RMB2685.00

Snk 抗体 (E-10) Alexa Fluor® 647

sc-374643 AF647
200 µg/ml
RMB2685.00

Snk 抗体 (E-10) Alexa Fluor® 680

sc-374643 AF680
200 µg/ml
RMB2685.00

Snk 抗体 (E-10) Alexa Fluor® 790

sc-374643 AF790
200 µg/ml
RMB2685.00

Snk (E-10) 中和勝肽

sc-374643 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-374643 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Fine for WBTested in SHSY-5Y human neuroblastoma cells (~15ug/lane), dilution 1:200 in 3% milk in TBST. Got a single clear band at expected size.
Date published: 2018-05-15
Rated 5 out of 5 by from Strong signalThis antibody will provide a strong and specific signal in mouse, rat, and human samples. The WB bands are of the expected weight and staining is clear.
Date published: 2017-02-08
Rated 5 out of 5 by from Excellent antibody for Western blottingThis product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a very potent and highly specific antibody for Western blotting with a mixture of mouse brain, testes and thymus tissue lysates. It very strongly detected an appropriately sized ~75 kDa target protein in these immunoblots.
Date published: 2016-10-13
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmicProduced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2015-01-12
Rated 5 out of 5 by from Nice Western Blot data of Snk expression in T24Nice Western Blot data of Snk expression in T24, ES-2, MCF7 and HISM whole cell lysates. -SCBT QC
Date published: 2014-10-26
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Snk 抗体 (E-10) is rated 5.0 out of 5 by 5.
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