Date published: 2025-9-7

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SMP30 抗体 (G-10): sc-374019

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说明书
  • SMP30抗体(G-10)是小鼠单克隆IgG1 κ, 在3篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的SMP30的内部区域内的氨基酸247-279之间
  • 抗-SMP30 抗体 (G-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 SMP30
  • 抗SMP30抗体(G-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SMP30 (G-10): sc-374019免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 SMP30 抗体 (G-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SMP30 抗体 (G-10) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

SMP30抗体(G-10)是一种IgG1 κ小鼠单克隆SMP30抗体,可通过WB、IP、IF、IHC(P)和ELISA技术检测小鼠、大鼠和人类的SMP30。SMP30抗体(G-10)既可以是非偶联的抗SMP30抗体形式,也可以是多种偶联形式的抗SMP30抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。衰老标志蛋白-30(SMP30)在肝脏、肾脏和颌下腺中表达。在肾脏中,SMP30定位于肝细胞和肾近端小管上皮。SMP30的表达水平在发育过程中的组织成熟期间增加,并以不依赖雄激素的方式随年龄增长而减少。SMP30通过调节质膜钙泵的活性影响细胞内钙稳态。SMP30对钙水平的影响似乎可以保护细胞免受凋亡。小鼠SMP30基因的启动子序列包含未知和已知转录因子的结合位点,包括Sp1、AP2、CCAAT框、Lyf-1和GATA-1。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SMP30 抗体 (G-10) 参考文献:

  1. 鉴定与小鼠衰老标记蛋白-30 基因启动子相互作用的新型序列特异性核因子  |  Supakar, PC., et al. 2000. Biochem Biophys Res Commun. 272: 436-40. PMID: 10833432
  2. 衰老标记蛋白-30基因敲除的小鼠肝脏极易受到肿瘤坏死因子-α和Fas介导的细胞凋亡的影响。  |  Ishigami, A., et al. 2002. Am J Pathol. 161: 1273-81. PMID: 12368201
  3. [衰老标记蛋白-30(SMP30)在小鼠下颌下腺中的定位及 SMP30 基因敲除小鼠的组织学发现]。  |  Ishii, K., et al. 2002. Nihon Jibiinkoka Gakkai Kaiho. 105: 915-9. PMID: 12400167
  4. 编码大鼠衰老标志蛋白-30(SMP30)的 cDNA 克隆的分离及其组织分布。  |  Fujita, T., et al. 1992. Biochim Biophys Acta. 1132: 297-305. PMID: 1420310
  5. 大鼠肝脏中衰老标志蛋白-30(SMP30)的纯化及其与雄激素无关的随年龄增长而减少的现象。  |  Fujita, T., et al. 1992. Biochim Biophys Acta. 1116: 122-8. PMID: 1581340
  6. 衰老的人类晶状体上皮细胞中 SMP30 的下调。  |  Li, S., et al. 2017. Mol Med Rep. 16: 4022-4028. PMID: 28765965
  7. 肝细胞癌血清 SMP30 和抗 SMP30 抗体的诊断价值  |  Zheng, SX., et al. 2018. Lab Med. 49: 203-210. PMID: 29562243
  8. 树突状细胞疫苗接种的 GP96 和 SMP30 蛋白引物可诱导更强的抗肝癌 CTL 反应]。  |  Huang, R., et al. 2022. J Healthc Eng. 2022: 2518847. PMID: 35070229
  9. 经肿瘤相关抗原SMP30修饰的树突状细胞在体外和体内对小鼠肝癌细胞的抗肿瘤效果增强。  |  Guo, J., et al. 2022. Am J Transl Res. 14: 5785-5799. PMID: 36105050
  10. 编码人类衰老标记蛋白-30(SMP30)同源物的 cDNA 克隆的分离及其在 X 染色体上的位置。  |  Fujita, T., et al. 1995. Biochim Biophys Acta. 1263: 249-52. PMID: 7548213
  11. 衰老标志蛋白-30(SMP30)的基因调控:随组织成熟而协调上调,随衰老而逐渐下调。  |  Fujita, T., et al. 1996. Mech Ageing Dev. 87: 219-29. PMID: 8794449
  12. 衰老标志蛋白-30(SMP30)通过增强肝 G2 细胞质膜 Ca(2+)-泵活性来挽救细胞死亡。  |  Fujita, T., et al. 1998. Biochem Biophys Res Commun. 250: 374-80. PMID: 9753637

订购信息

产品名称产品编号规格价格数量收藏夹

SMP30 抗体 (G-10)

sc-374019
200 µg/ml
RMB2377.00

SMP30 (G-10): m-IgG Fc BP-HRP 套装

sc-529635
200 µg Ab; 10 µg BP
RMB2662.00

SMP30 (G-10): m-IgGκ BP-HRP 套装

sc-522589
200 µg Ab, 40 µg BP
RMB2662.00

SMP30 抗体 (G-10) AC

sc-374019 AC
500 µg/ml, 25% agarose
RMB3129.00

SMP30 抗体 (G-10) HRP

sc-374019 HRP
200 µg/ml
RMB2377.00

SMP30 抗体 (G-10) FITC

sc-374019 FITC
200 µg/ml
RMB2482.00

SMP30 抗体 (G-10) PE

sc-374019 PE
200 µg/ml
RMB2580.00

SMP30 抗体 (G-10) Alexa Fluor® 488

sc-374019 AF488
200 µg/ml
RMB2685.00

SMP30 抗体 (G-10) Alexa Fluor® 546

sc-374019 AF546
200 µg/ml
RMB2685.00

SMP30 抗体 (G-10) Alexa Fluor® 594

sc-374019 AF594
200 µg/ml
RMB2685.00

SMP30 抗体 (G-10) Alexa Fluor® 647

sc-374019 AF647
200 µg/ml
RMB2685.00

SMP30 抗体 (G-10) Alexa Fluor® 680

sc-374019 AF680
200 µg/ml
RMB2685.00

SMP30 抗体 (G-10) Alexa Fluor® 790

sc-374019 AF790
200 µg/ml
RMB2685.00

SMP30 (G-10) 中和勝肽

sc-374019 P
100 µg/0.5 ml
RMB511.00

What is the different between SMP30 Antibody (17) and SMP30 Antibody (G-10)? I would like to detect the SMP30 expression in liver and kidney of rats in both western blot and immunohistochemistry.  So, which product should be suitable for this detection?

Asked by: Primates
Thank you for your question. These are two different mouse monoclonal antibodies with distinct epitopes, both recognizing SMP30. Both antibodies are suitable for your intended research applications.
Answered by: Technical Support
Date published: 2019-01-26

这支抗体能检测smp30蛋白的其他亚型吗?WB、IP、IF、elisa、IHC、FCM真的都可以做吗?能否送一个试用装给我用一下?

Asked by: sunshine666
感谢您的提问。官网上说明的我们都是提供质保的,关于试用装的申请,欢迎通过:电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. For more detailed information about sample request, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-02-01

I used this Ab (sc-374019) to stain FFPE kidney sections (Rat) following  IF & IHC protocols but there was no staining (Antigen retrieval with 10 mM sodium citrate buffer, pH 6.0 at 100 °C,10 min).Do you recommend any special treatment for this antibody?

Asked by: GBar
Thank you for your question. For the IHC(P) data shown on our website using this antibody, SMP30 (G-10): sc-374019, we also used the same antigen retrieval method of sodium citrate buffer with heat treatment. If you have not seeing any staining, we would recommend using the primary antibody at the starting dilution of 1:50. A link to our complete recommended protocol is provided below. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining If you need assistance in troubleshooting the antibody, please contact our Technical Service Department. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-06-29

What application is the blocking peptide sc-374019 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 使用感3年前からdailyにWBに使用しています。 培養細胞および、ラット・マウスの組織においても、選択性が高く、良い抗体だと思います。
Date published: 2021-04-27
Rated 4 out of 5 by from good IHC stainingI got good IHC staining result with DAB in mouse liver
Date published: 2019-11-22
Rated 5 out of 5 by from GreatIt is reactive with mouse, rat, and human, bands were clear.
Date published: 2017-05-16
Rated 5 out of 5 by from Wise choice in antibody...This is a strong SMP30 clone, clean specific and reliable. Great addition to any lab working with SMP30!
Date published: 2017-02-02
Rated 5 out of 5 by from Beautiful immunoperoxidase cytoplasmic andBeautiful immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
Date published: 2015-01-20
Rated 5 out of 5 by from Great Western Blot data of SMP30 expressionGreat Western Blot data of SMP30 expression in Hep G2 whole cell lysate. -SCBT QC
Date published: 2013-02-08
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SMP30 抗体 (G-10) is rated 4.8 out of 5 by 6.
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