Date published: 2025-9-7

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smoothelin 抗体 (C-8): sc-376902

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说明书
  • smoothelin 抗体 C-8 是小鼠单克隆 IgG2b κ,smoothelin抗体, 在8篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的smoothelin的C-terminus附近的氨基酸831-869之间
  • smoothelin 抗体 (C-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 smoothelin long and short isoforms; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 抗smoothelin抗体(C-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取smoothelin (C-8): sc-376902免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 smoothelin 抗体 (C-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与smoothelin 抗体 (C-8) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

smoothelin抗体(C-8)是一种IgG2b κ小鼠单克隆smoothelin抗体(也称为SMTN蛋白抗体或SMSMO抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的smoothelin蛋白。smoothelin抗体(C-8)既可作为非偶联的抗smoothelin抗体形式提供,也可作为多种偶联形式的抗smoothelin抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。细胞骨架蛋白smoothelin在脊椎动物中高度保守,仅由收缩性平滑肌细胞表达,并定位于丝状网络中。Smoothelin与肌动蛋白应力纤维相关,但不与结蛋白相互作用。通过选择性剪接至少产生两种smoothelin同工型。短同工型在长同工型的氨基末端缺少1-456个氨基酸。短同工型在包括肠和胃在内的内脏肌肉组织中表达,但不在脑中表达,而长同工型则在所有血管化器官中表达。在血管系统中,smoothelin的表达仅限于能够进行脉动收缩的大静脉和动脉。作为高度分化的收缩性平滑肌细胞的标记物,smoothelin的表达对于研究血管畸形和损伤很有用。编码人smoothelin的基因定位于22号染色体q12.2区。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

smoothelin 抗体 (C-8) 参考文献:

  1. 平滑肌细胞分化标志物平滑素的一种新型同源物。  |  Krämer, J., et al. 1999. J Mol Med (Berl). 77: 294-8. PMID: 10023782
  2. 平滑素表达的增加可模拟妊娠期人类子宫肌层中刺激性G蛋白α亚基的明显上调。  |  Gsell, S., et al. 2000. FASEB J. 14: 17-26. PMID: 10627276
  3. 小鼠平滑素基因Smtn的结构和染色体定位。  |  Rensen, S., et al. 2000. Cytogenet Cell Genet. 89: 225-9. PMID: 10965129
  4. 平滑肌收缩性调节,一种平滑素家族蛋白的新成员。  |  Borman, MA., et al. 2004. FEBS Lett. 573: 207-13. PMID: 15327999
  5. 类平滑肌蛋白 1 的钙蛋白同源(CH)结构域的溶液结构:独特的 apocalmodulin 结合模式以及 C 端 2 型 CH 结构域在平滑肌松弛中的可能作用。  |  Ishida, H., et al. 2008. J Biol Chem. 283: 20569-78. PMID: 18477568
  6. 膀胱平滑肌和肌成纤维细胞中免疫组织化学标记物的差异表达,以及肌动蛋白、平滑素和波形蛋白在膀胱癌分期中的潜在用途。  |  Council, L. and Hameed, O. 2009. Mod Pathol. 22: 639-50. PMID: 19252475
  7. 作为响应,我们改进了Smoothelin免疫染色的染色方案。  |  Chakravarthy, R., et al. 2011. Virchows Arch. 459: 119-20. PMID: 21638012
  8. 哮喘患者原发性支气管成纤维细胞细胞特征的初步研究:α-平滑肌肌动蛋白、含有额外III型结构域A的纤连蛋白和平滑素表达。  |  Nam, YH., et al. 2012. J Investig Allergol Clin Immunol. 22: 20-7. PMID: 22448450
  9. 平滑素样蛋白1与原肌球蛋白和钙调蛋白的结合是相互排斥的,并且受磷酸化调节。  |  Ulke-Lemée, A., et al. 2017. BMC Biochem. 18: 5. PMID: 28320308
  10. 通过荧光原位杂交和辐射杂交定位,将平滑素(SMTN)的人类基因分配到22q12染色体上。  |  Engelen, JJ., et al. 1997. Genomics. 43: 245-7. PMID: 9244445

订购信息

产品名称产品编号规格价格数量收藏夹

smoothelin 抗体 (C-8)

sc-376902
200 µg/ml
RMB2377.00

smoothelin (C-8): m-IgGκ BP-HRP 套装

sc-523131
200 µg Ab, 40 µg BP
RMB2662.00

smoothelin (C-8): m-IgG2b BP-HRP 套装

sc-549090
200 µg Ab; 10 µg BP
RMB2662.00

smoothelin 抗体 (C-8) AC

sc-376902 AC
500 µg/ml, 25% agarose
RMB3129.00

smoothelin 抗体 (C-8) HRP

sc-376902 HRP
200 µg/ml
RMB2377.00

smoothelin 抗体 (C-8) FITC

sc-376902 FITC
200 µg/ml
RMB2482.00

smoothelin 抗体 (C-8) PE

sc-376902 PE
200 µg/ml
RMB2580.00

smoothelin 抗体 (C-8) Alexa Fluor® 488

sc-376902 AF488
200 µg/ml
RMB2685.00

smoothelin 抗体 (C-8) Alexa Fluor® 546

sc-376902 AF546
200 µg/ml
RMB2685.00

smoothelin 抗体 (C-8) Alexa Fluor® 594

sc-376902 AF594
200 µg/ml
RMB2685.00

smoothelin 抗体 (C-8) Alexa Fluor® 647

sc-376902 AF647
200 µg/ml
RMB2685.00

smoothelin 抗体 (C-8) Alexa Fluor® 680

sc-376902 AF680
200 µg/ml
RMB2685.00

smoothelin 抗体 (C-8) Alexa Fluor® 790

sc-376902 AF790
200 µg/ml
RMB2685.00

smoothelin (C-8) 中和勝肽

sc-376902 P
100 µg/0.5 ml
RMB511.00

Qual o protocolo (fixação, duração do bloqueio, duração da incubação) é a mais correta para este anticorpo? É que utilizei o protocolo que costumo usar para os restantes anticorpos e não marcou nada (com a diluição recomendada na datasheet)

Asked by: MGOP
Thank you for your question. The recommended protocol is on our website. Please contact our Technical Service Department for assistance with troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-09-11

What application is the blocking peptide sc-376902 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Got a nice result in immunofluorescent stainingAs recommend, I used 1:50 in smooth muscle cell, and shoot some beautiful images by confocol. A great experiment experience.
Date published: 2017-10-24
Rated 5 out of 5 by from Very Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-02-07
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human esophagus tissue. -SCBT QC
Date published: 2015-02-18
Rated 5 out of 5 by from Nice Western Blot data of smoothelin expressionNice Western Blot data of smoothelin expression in non-transfected and human smoothelin transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-09-23
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smoothelin 抗体 (C-8) is rated 5.0 out of 5 by 4.
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