Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Smooth Muscle Actin Antibody (B4) - Western Blotting - Image 41390
平滑肌肌动蛋白抗体 (B4): sc-53142. 未转染 293T: sc-117752 (A), 转染小鼠 ACTG1 的 293T: sc-124922 (B), HeLa (C) 和 A-431 (D) 全细胞裂解液中 ACTG1 表达的 Western 印迹分析.
平滑肌肌动蛋白抗体 (B4): sc-53142. 未转染 293T: sc-117752 (A), 转染小鼠 ACTG1 的 293T: sc-124922 (B), HeLa (C) 和 A-431 (D) 全细胞裂解液中 ACTG1 表达的 Western 印迹分析.

Smooth Muscle Actin 抗体 (B4): sc-53142

4.7(21)
寫評論提問

说明书
  • Smooth Muscle Actin 抗体 B4 是小鼠单克隆 IgG1 κ，Smooth Muscle Actin抗体, 在263篇文献中引用，规格为200 µg/ml
  • 针对禽肫肌动蛋白
  • Smooth Muscle Actin 抗体 (B4) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human 和chicken 来源的 enteric smooth muscle γ-Actin and, to a lesser extent, α-smooth muscle, α-skeletal and α-cardiac (sarcomeric) isoforms of Actin; 不会与 cytoplasmic Actins产生交联反应
  • 抗Smooth Muscle Actin抗体（B4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Smooth Muscle Actin (B4): sc-53142的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Smooth Muscle Actin 抗体 (B4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Smooth Muscle Actin 抗体 (B4) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Actin 抗体 包括 α-Actin, β-Actin, γ-Actin, Muscle Actin和Smooth Muscle Actin

平滑肌肌动蛋白抗体（B4）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学等方法检测小鼠、大鼠、人类和鸡样本中的平滑肌肌动蛋白。抗平滑肌肌动蛋白抗体（B4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。平滑肌肌动蛋白在肌肉收缩和细胞运动中起着关键作用，是平滑肌细胞收缩装置的重要组成部分，用于调节血管收缩和胃肠蠕动等各种生理过程。平滑肌肌动蛋白维持平滑肌组织的结构完整性和功能，其表达在发育过程中受到严格调控，并响应各种刺激。平滑肌肌动蛋白有助于压力纤维的形成，对维持细胞形状和促进细胞运动至关重要。了解平滑肌肌动蛋白的动态变化及其与肌球蛋白等其他蛋白的相互作用有助于阐明平滑肌功能和疾病病理生理学机制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Smooth Muscle Actin 抗体 (B4) 参考文献:

  1. 哺乳动物胚胎发育过程中平滑肌肌动蛋白的细胞分布：在分化的横纹肌细胞中表达α-血管异构体，而不是γ-肠管异构体。  |  Sawtell, NM. and Lessard, JL. 1989. J Cell Biol. 109: 2929-37. PMID: 2687290
  2. 血管病变中的α-平滑肌肌动蛋白和ACTA2基因表达。  |  Yuan, SM. 2015. Braz J Cardiovasc Surg. 30: 644-9. PMID: 26934405
  3. α-平滑肌肌动蛋白在成纤维细胞介导的基质收缩和重塑中的作用  |  Shinde, AV., et al. 2017. Biochim Biophys Acta Mol Basis Dis. 1863: 298-309. PMID: 27825850
  4. 平滑肌肌动蛋白作为实验鼠胃和空肠严重敏感性的标准。  |  Samoilenko, T., et al. 2023. Int J Mol Sci. 24: PMID: 38003728
  5. Claudin 7通过抑制平滑肌肌动蛋白程序抑制侵袭和转移。  |  West, JJ., et al. 2024. J Cell Biol. 223: PMID: 39320351
  6. 微管相关蛋白在控制微管组装中的作用  |  Maccioni, RB. and Cambiazo, V. 1995. Physiol Rev. 75: 835-64. PMID: 7480164
  7. Rho, rac和cdc42 GTP酶调节与肌动蛋白应力纤维, 薄片和丝状体相关的多分子缔合作用。  |  Nobes, CD. and Hall, A. 1995. Cell. 81: 53-62. PMID: 7536630
  8. 关于 F-肌动蛋白建模的讨论。  |  Schutt, CE., et al. 1995. J Struct Biol. 115: 186-98. PMID: 7577239
  9. 肌动蛋白细胞骨架设定细胞运动的节奏  |  Barkalow, K. and Hartwig, JH. 1995. Curr Biol. 5: 1000-2. PMID: 8542272
  10. 真核生物蛋白质的起源和进化。  |  Doolittle, RF. 1995. Philos Trans R Soc Lond B Biol Sci. 349: 235-40. PMID: 8577833
  11. 弹性纤维是人类胎盘干绒毛基质的重要组成部分，也是血管周围收缩鞘的组成部分。  |  Graf, R., et al. 1996. Cell Tissue Res. 283: 133-41. PMID: 8581953
  12. 激光共聚焦显微镜揭示的人类毛囊中的肌动蛋白束。  |  Furumura, M. and Ishikawa, H. 1996. Cell Tissue Res. 283: 425-34. PMID: 8593672

订购信息

产品名称产品编号规格价格数量收藏夹

Smooth Muscle Actin 抗体 (B4)

sc-53142
200 µg/ml
RMB2377.00

Smooth Muscle Actin (B4): m-IgG Fc BP-HRP 套装

sc-528554
200 µg Ab; 10 µg BP
RMB2662.00

Smooth Muscle Actin (B4): m-IgGκ BP-HRP 套装

sc-520954
200 µg Ab, 40 µg BP
RMB2662.00

Smooth Muscle Actin (B4): m-IgG1 BP-HRP 套装

sc-542991
200 µg Ab; 20 µg BP
RMB2662.00

Smooth Muscle Actin 抗体 (B4) AC

sc-53142 AC
500 µg/ml, 25% agarose
RMB3129.00

Smooth Muscle Actin 抗体 (B4) HRP

sc-53142 HRP
200 µg/ml
RMB2377.00

Smooth Muscle Actin 抗体 (B4) FITC

sc-53142 FITC
200 µg/ml
RMB2482.00

Smooth Muscle Actin 抗体 (B4) PE

sc-53142 PE
200 µg/ml
RMB2580.00

Smooth Muscle Actin 抗体 (B4) Alexa Fluor® 488

sc-53142 AF488
200 µg/ml
RMB2685.00

Smooth Muscle Actin 抗体 (B4) Alexa Fluor® 546

sc-53142 AF546
200 µg/ml
RMB2685.00

Smooth Muscle Actin 抗体 (B4) Alexa Fluor® 594

sc-53142 AF594
200 µg/ml
RMB2685.00

Smooth Muscle Actin 抗体 (B4) Alexa Fluor® 647

sc-53142 AF647
200 µg/ml
RMB2685.00

Smooth Muscle Actin 抗体 (B4) Alexa Fluor® 680

sc-53142 AF680
200 µg/ml
RMB2685.00

Smooth Muscle Actin 抗体 (B4) Alexa Fluor® 790

sc-53142 AF790
200 µg/ml
RMB2685.00

Is this product's host species chicken?

Asked by: Vmmm
This antibody is raised in a mouse cell line.
Answered by: Dallas Tech Service
Date published: 2021-12-10

I want to detect smooth muscle actin in my sheep samples, will this antibody work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2023-11-09
  • y_2025, m_9, d_6, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_53142, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 119ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Good for IFProvided a strong signal in immunofluorescence of human fibroblasts (needs to be used in methanol-fixed cells).
Date published: 2023-10-15
Rated 5 out of 5 by from Excellent antibody for multiple speciesI have used this antibody in tissues of various animal species blocked in paraffin fixed with formol. As antigen detection, I applied microwave in 20 minutes in Citrate buffer, pH 6.0 solution. the results were quite satisfactory for each species.
Date published: 2023-05-07
Rated 5 out of 5 by from Excellent staining in paraffin sectionsObtuvimos la muestra el mes pasado unos resultados muy buenos en muestras de parafina, tanto de origen de ratón como de humano.
Date published: 2021-08-09
Rated 5 out of 5 by from For western blot is okThe western blot is ok, but only with some conditions. My best conditions are Blocking 5% milk 30 minutes I antibody 1:500 on in tTbs II antibody 1:2000 mouse in 3%milk
Date published: 2019-09-18
Rated 4 out of 5 by from OKLa banda de 43 KDa sale bien a la dilución indicada. Sin embargo, también aparece una banda inespecífica en torno a 55 KDa con la misma intensidad que la de 43 KDa
Date published: 2018-03-20
Rated 5 out of 5 by from Beta actin for western blotNormalization by B-actin (sc-53142) is very good in chicken, clear bands at high antibody dilution and very low background reaction. can be incubated easily with other antibodies with no problem.
Date published: 2018-02-20
Rated 5 out of 5 by from Very good, specific antibodyI used this antibody for western blotting and immunofluorescence in primary cultured adipocytes. Only one specificband showed up in WB, immnofluorescence is specific to cytoplasm.
Date published: 2018-02-14
Rated 2 out of 5 by from Ziemlich schwaches Signal in IFDer Antikörper liefert in humenen Fibroblasten recht schwache zytoplasmatische Signale
Date published: 2018-01-17
  • y_2025, m_9, d_6, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_21
  • loc_zh_CN, sid_53142, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT
Smooth Muscle Actin抗体 (B4) is rated 4.7 out of 5 by 21.
  • y_2025, m_9, d_6, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_21
  • loc_zh_CN, sid_53142, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 133ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。