Date published: 2025-9-7

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SmarcAL1 抗体 (A-2): sc-376377

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说明书
  • SmarcAL1 抗体 A-2 是小鼠单克隆 IgG1 κ,SmarcAL1抗体, 在32篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列831-954位于human物种的SmarcAL1的C-terminus
  • SmarcAL1 抗体 (A-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 SmarcAL1
  • 抗SmarcAL1抗体(A-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SmarcAL1 (A-2): sc-376377免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 SmarcAL1 抗体 (A-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SmarcAL1 抗体 (A-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

SmarcAL1抗体(A-2)是一种IgG1 κ小鼠单克隆SmarcAL1抗体(也称为SmarcAL1抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的SmarcAL1蛋白。SmarcAL1抗体(A-2)既可以作为非偶联的抗SmarcAL1抗体形式提供,也可以作为多种偶联形式的抗SmarcAL1抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。SmarcAL1(SWI/SNF相关基质相关肌动蛋白依赖性染色质亚家族A样蛋白1调节因子),也称为HARP(HepA相关蛋白)或HHARP,是SWI/SNF家族解旋酶和ATP酶蛋白的954个氨基酸成员。SmarcAL1定位于细胞核,是一种普遍表达的蛋白质,在ATP依赖性核小体重塑活动中发挥作用。SmarcAL1含有一个保守的C端SNF2结构域、一个解旋酶ATP结合结构域和两个HARP结构域。编码SmarcAL1的基因缺陷是导致Schimke免疫骨发育不良(SIOD)的原因,这是一种常染色体隐性遗传病,其特征是肾功能不全、脊椎干骺端发育不良和T细胞免疫缺陷。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SmarcAL1 抗体 (A-2) 参考文献:

  1. 染色质重塑蛋白 SMARCAL1 突变导致席姆克免疫骨发育不良。  |  Boerkoel, CF., et al. 2002. Nat Genet. 30: 215-20. PMID: 11799392
  2. 施姆克免疫骨发育不良症患者的寿命。  |  Lou, S., et al. 2002. J Med Genet. 39: 922-5. PMID: 12471207
  3. Schimke-免疫骨发育不良:兄弟姐妹中基因型与表型相关性弱的新突变。  |  Lücke, T., et al. 2005. Am J Med Genet A. 135: 202-5. PMID: 15880370
  4. 偏头痛样头痛与施姆克免疫骨发育不良症有关。  |  Kilic, SS., et al. 2005. Am J Med Genet A. 135: 206-10. PMID: 15884045
  5. 与轻度席姆克免疫骨发育不良有关的 SMARCAL1 基因 R561C 缺失突变。  |  Bökenkamp, A., et al. 2005. Pediatr Nephrol. 20: 1724-8. PMID: 16237566
  6. 希姆克免疫骨发育不良:一种细胞自主性疾病?  |  Elizondo, LI., et al. 2006. Am J Med Genet A. 140: 340-8. PMID: 16419127
  7. 施姆克免疫性骨发育不良症:遗传多样性的建议。  |  Clewing, JM., et al. 2007. Hum Mutat. 28: 273-83. PMID: 17089404
  8. 从 HARP C 端提取的基本肽可抑制 DU145 前列腺癌细胞的锚定依赖性生长。  |  Bermek, O., et al. 2007. Exp Cell Res. 313: 4041-50. PMID: 17727841
  9. 克隆和鉴定 HARP/SMARCAL1:一种来自人类和小鼠的原核 HepA 相关 SNF2 螺旋酶蛋白。  |  Coleman, MA., et al. 2000. Genomics. 65: 274-82. PMID: 10857751

订购信息

产品名称产品编号规格价格数量收藏夹

SmarcAL1 抗体 (A-2)

sc-376377
200 µg/ml
RMB2377.00

SmarcAL1 (A-2): m-IgG Fc BP-HRP 套装

sc-529863
200 µg Ab; 10 µg BP
RMB2662.00

SmarcAL1 (A-2): m-IgGκ BP-HRP 套装

sc-522935
200 µg Ab, 40 µg BP
RMB2662.00

SmarcAL1 (A-2): m-IgG1 BP-HRP 套装

sc-543732
200 µg Ab; 20 µg BP
RMB2662.00

SmarcAL1 抗体 (A-2) AC

sc-376377 AC
500 µg/ml, 25% agarose
RMB3129.00

SmarcAL1 抗体 (A-2) HRP

sc-376377 HRP
200 µg/ml
RMB2377.00

SmarcAL1 抗体 (A-2) FITC

sc-376377 FITC
200 µg/ml
RMB2482.00

SmarcAL1 抗体 (A-2) PE

sc-376377 PE
200 µg/ml
RMB2580.00

SmarcAL1 抗体 (A-2) Alexa Fluor® 488

sc-376377 AF488
200 µg/ml
RMB2685.00

SmarcAL1 抗体 (A-2) Alexa Fluor® 546

sc-376377 AF546
200 µg/ml
RMB2685.00

SmarcAL1 抗体 (A-2) Alexa Fluor® 594

sc-376377 AF594
200 µg/ml
RMB2685.00

SmarcAL1 抗体 (A-2) Alexa Fluor® 647

sc-376377 AF647
200 µg/ml
RMB2685.00

SmarcAL1 抗体 (A-2) Alexa Fluor® 680

sc-376377 AF680
200 µg/ml
RMB2685.00

SmarcAL1 抗体 (A-2) Alexa Fluor® 790

sc-376377 AF790
200 µg/ml
RMB2685.00

I am using SmarcAL1 (A-2): sc-376377 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works for western blots on leukemia cell linesThe antibody is effective for SDS immunoblotting. To dilute the primary antibody, we used a ratio of 1:500 in a mixture of 5% nonfat dry milk, 1X TBS, and 0.1% Tween® 20. The antibody was left overnight at 4°C.
Date published: 2023-11-07
Rated 4 out of 5 by from Works for WesternA Post-doc in our lab used this at 1:500 with mouse ES cell whole cell lysates (40 ug / lane). It worked! Decent signal and no cross-reacting/non-specific bands.
Date published: 2018-09-13
Rated 5 out of 5 by from Recommended for IHC(P)Produced beautiful nuclear and cytoplasmic immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue
Date published: 2017-01-24
Rated 4 out of 5 by from Good Antibody for WBGood antibody in WB, a single clean band. Sadly, doesn't work in IF even with 3 different protocols.
Date published: 2016-12-07
Rated 4 out of 5 by from Bon anticorps pour WBBon anticorps, facilement detectable à 1:1000, pas de bruit de fond ou de bandes non-spécifiques.
Date published: 2016-11-18
Rated 4 out of 5 by from Very Good antibody for IF and WB but not IPVery Good antibody for IF and WB but not IP.
Date published: 2016-06-09
Rated 5 out of 5 by from Produced excellent immunofluorescence nuclearProduced excellent immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-04-20
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SmarcAL1 抗体 (A-2) is rated 4.5 out of 5 by 8.
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