Date published: 2025-9-6
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Smad2/3 Antibody (C-8) - Western Blotting - Image 59787
Smad2/3 抗体 (C-8): sc-133098. K-562 (A), U-937 (B), KNRK (C) 和 NIH/3T3 (D) 全细胞裂解液中 Smad2/3 表达的 Western 印迹分析.
Smad2/3 抗体 (C-8): sc-133098. K-562 (A), U-937 (B), KNRK (C) 和 NIH/3T3 (D) 全细胞裂解液中 Smad2/3 表达的 Western 印迹分析.

Smad2/3 抗体 (C-8): sc-133098

4.8(42)
寫評論提問

说明书
  • Smad2/3抗体(C-8)是小鼠单克隆IgG2a κ, 在126篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1-425的抗原，代表全长Smad3（human起源
  • 抗-Smad2/3 抗体 (C-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Smad2 and Smad3; 也和以下物种反应，包括： 和 canine and porcine
  • 抗Smad2/3抗体（C-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-133098 X, 200 µg/0.1 ml)
  • 关于如何获取Smad2/3 (C-8): sc-133098的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 Smad2/3 抗体 (C-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Smad2/3 抗体 (C-8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加Smad抗体，包括Smad1, Smad2, Smad3, Smad4, Smad5, Smad6, Smad7和Smad8

Smad2/3抗体（C-8）是一种IgG2a κ小鼠单克隆Smad2/3抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的Smad2和Smad3。Smad2/3抗体（C-8）既可以是非偶联的抗Smad2/3抗体形式，也可以是多种偶联形式的抗Smad2/3抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Smad蛋白是果蝇mothers against decapentaplegic（Mad）的哺乳动物同源物，已被证实为TGFβ/BMP信号的下游效应器。Smad1（也称为Madr1或JV4-1）和Smad5是BMP-2和BMP-4功能的效应器，而Smad2（也称为Madr2或JV18-1）和Smad3则参与TGFβ和激活素介导的生长调节。已证实Smad4（也称为DPC4）通过与各种Smad家族成员相互作用来介导上述所有活动。Smad6和Smad7通过干扰TGFβ介导的其他Smad蛋白的磷酸化来调节对激活素/TGFβ信号的应答。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Smad2/3 抗体 (C-8)

sc-133098
200 µg/ml
RMB2377.00

Smad2/3 (C-8): m-IgG Fc BP-HRP 套装

sc-528750
200 µg Ab; 10 µg BP
RMB2662.00

Smad2/3 (C-8): m-IgGκ BP-HRP 套装

sc-521235
200 µg Ab, 40 µg BP
RMB2662.00

Smad2/3 抗体 (C-8) X

sc-133098 X
200 µg/0.1 ml
RMB2377.00

Smad2/3 抗体 (C-8) AC

sc-133098 AC
500 µg/ml, 25% agarose
RMB3129.00

Smad2/3 抗体 (C-8) HRP

sc-133098 HRP
200 µg/ml
RMB2377.00

Smad2/3 抗体 (C-8) FITC

sc-133098 FITC
200 µg/ml
RMB2482.00

Smad2/3 抗体 (C-8) PE

sc-133098 PE
200 µg/ml
RMB2580.00

Smad2/3 抗体 (C-8) Alexa Fluor® 488

sc-133098 AF488
200 µg/ml
RMB2685.00

Smad2/3 抗体 (C-8) Alexa Fluor® 546

sc-133098 AF546
200 µg/ml
RMB2685.00

Smad2/3 抗体 (C-8) Alexa Fluor® 594

sc-133098 AF594
200 µg/ml
RMB2685.00

Smad2/3 抗体 (C-8) Alexa Fluor® 647

sc-133098 AF647
200 µg/ml
RMB2685.00

Smad2/3 抗体 (C-8) Alexa Fluor® 680

sc-133098 AF680
200 µg/ml
RMB2685.00

Smad2/3 抗体 (C-8) Alexa Fluor® 790

sc-133098 AF790
200 µg/ml
RMB2685.00

I want to detect SMAD2/3 phosphorylated protein expression in porcine cells. But I'm not found this criteria in primary antibody of Santa cruz. I found that p-Smad2/ 3 (Ser 423/425) is not available now. Can you suggest me which one is proper?

Asked by: Ploy
Thank you for your question. Unfortunately we do not have a anti-phosphorylated SMAD antibody at this time. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-06-20

I WOULD LIKE TO PURCHASE ANTIBODY AGAINST PHOSPHORLYATED SMAD1 AN SMAD2 AND THEIR SECONDARY ANTIBODY FOR WESTERN BLOT

Asked by: MM ALHARBI
Thank you for your question. Unfortunately, we do not currently offer any phospho-specific Smad1 or Smad2 antibodies. This mouse monoclonal, Smad2/3 Antibody (C-8): sc-133098, is against total Smad2/3 and may detect both the phosphorylated and non-phosphorylated forms of the proteins.
Answered by: Technical Support
Date published: 2017-08-22

Can Smad2/3 (C-8): sc-133098 monoclonal antibody be used for detection of Smad2/3 of equine origin?

Asked by: jenni
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 14
Date published: 2017-01-11
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Rated 5 out of 5 by from Works well for WBWorks well on neonatal mouse lungs at concentration of 30ug. Ab dilution will be 1:200 for overnight @ 4oC.
Date published: 2024-12-10
Rated 5 out of 5 by from Smad 2/3I tested this antibody in western blotting (1:200) and I am very satisfied.
Date published: 2024-01-26
Rated 5 out of 5 by from This antibody is very good!The bands bound with this antibody are very well exposed. I purchased this antibody last year and performed western blot detection on the cell protein samples collected from the RIPA lysate, and the background was very clean.
Date published: 2022-10-08
Rated 5 out of 5 by from The antibody was very goodWhen I use the antibody, the bands were easy to visualize and very clear.
Date published: 2022-07-29
Rated 4 out of 5 by from Good in immunofluorescenceGood resuts have been achieved in immunofluorescence (1:200) on mesenchymal stem cells fixed with 4% PFA.
Date published: 2020-11-10
Rated 4 out of 5 by from Buena marcaObtuve buena marca, debo corregir el inespecifico o diluir el acpo
Date published: 2019-03-14
Rated 5 out of 5 by from Nice and strong band (w.b.)Produced a single band at the expected molecular weight (~55kDa). Sample: MDA-MB-231 wcl (30ug). Blocking: 1h in blocking buffer (0.05% tween-20 in TBS with 5% skin milk). Primary antibody: 1:200 in blocking buffer, overnight @ 4oC. Secondary antibody: 1:3000 in blocking buffer. Exposure time: 5 seconds* I'm certainly very satisfied with this product. *further dilution of the primary antibody, maybe even at 1:1000, will likely yield similar results at the expense of a prolonged (and more adequate) exposure time.
Date published: 2018-11-30
Rated 5 out of 5 by from my review for Smad2/3 antibody (sc-133098)I tested a free antibody for Smad2/3 antibody (sc-133098) using IP samples from mouse fibrotic kidneys for Western blot. We loaded 80 ug proteins in each slot in the SDS gel. The antibody was diluted in 1:500 in 3% BSA/PBST and incubated overnight with the blot. We can observe a very clear band around 55-60 kd. We have repeated this experiment several times and used the fresh diluted antibody on the fresh blot.
Date published: 2018-05-14
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Smad2/3抗体 (C-8) is rated 4.8 out of 5 by 42.
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