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Smad2/3 Antibody (A-3) - Western Blotting - Image 309643
Smad2/3 抗体 (A-3): sc-398844. KNRK (A), Jurkat (B), K-562 (C), NIH/3T3 (D), C2C12 (E) 和 HeLa (F) 全细胞裂解液中 Smad2/3 表达的 Western 印迹分析.
Smad2/3 抗体 (A-3): sc-398844. KNRK (A), Jurkat (B), K-562 (C), NIH/3T3 (D), C2C12 (E) 和 HeLa (F) 全细胞裂解液中 Smad2/3 表达的 Western 印迹分析.

Smad2/3 抗体 (A-3): sc-398844

4.8(13)
寫評論提問

说明书
  • Smad2/3抗体(A-3)是小鼠单克隆IgG2b κ, 在16篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Smad3的N-terminus附近的氨基酸145-164之间
  • 抗-Smad2/3 抗体 (A-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Smad2 and Smad3
  • 抗Smad2/3抗体（A-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-398844 X, 200 µg/0.1 ml)
  • 关于如何获取Smad2/3 (A-3): sc-398844的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2b BP-HRP">m-IgG2b BP-HRP是Smad2/3 Antibody (A-3) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂，这些试剂现与Smad2/3 Antibody (A-3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加Smad抗体，包括Smad1, Smad2, Smad3, Smad4, Smad5, Smad6, Smad7和Smad8

Smad2/3抗体（A-3）是一种IgG2b κ小鼠单克隆Smad2/3抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的Smad2和Smad3。Smad2/3抗体（A-3）既可以是非偶联的抗Smad2/3抗体形式，也可以是多种偶联形式的抗Smad2/3抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Smad蛋白是果蝇mothers against decapentaplegic（Mad）的哺乳动物同源物，已被证实为TGFβ/BMP信号的下游效应器。Smad1（也称为Madr1或JV4-1）和Smad5是BMP-2和BMP-4功能的效应器，而Smad2（也称为Madr2或JV18-1）和Smad3则参与TGFβ和激活素介导的生长调节。已证实Smad4（也称为DPC4）通过与各种Smad家族成员相互作用来介导上述所有活动。Smad6和Smad7通过干扰TGFβ介导的其他Smad蛋白的磷酸化来调节对激活素/TGFβ信号的应答。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Smad2/3 抗体 (A-3)

sc-398844
200 µg/ml
RMB2377.00

Smad2/3 (A-3): m-IgG Fc BP-HRP 套装

sc-526327
200 µg Ab; 10 µg BP
RMB2662.00

Smad2/3 (A-3): m-IgG2b BP-HRP 套装

sc-549714
200 µg Ab; 10 µg BP
RMB2662.00

Smad2/3 抗体 (A-3) X

sc-398844 X
200 µg/0.1 ml
RMB2377.00

Smad2/3 抗体 (A-3) AC

sc-398844 AC
500 µg/ml, 25% agarose
RMB3129.00

Smad2/3 抗体 (A-3) HRP

sc-398844 HRP
200 µg/ml
RMB2377.00

Smad2/3 抗体 (A-3) FITC

sc-398844 FITC
200 µg/ml
RMB2482.00

Smad2/3 抗体 (A-3) PE

sc-398844 PE
200 µg/ml
RMB2580.00

Smad2/3 抗体 (A-3) Alexa Fluor® 488

sc-398844 AF488
200 µg/ml
RMB2685.00

Smad2/3 抗体 (A-3) Alexa Fluor® 546

sc-398844 AF546
200 µg/ml
RMB2685.00

Smad2/3 抗体 (A-3) Alexa Fluor® 594

sc-398844 AF594
200 µg/ml
RMB2685.00

Smad2/3 抗体 (A-3) Alexa Fluor® 647

sc-398844 AF647
200 µg/ml
RMB2685.00

Smad2/3 抗体 (A-3) Alexa Fluor® 680

sc-398844 AF680
200 µg/ml
RMB2685.00

Smad2/3 抗体 (A-3) Alexa Fluor® 790

sc-398844 AF790
200 µg/ml
RMB2685.00

What is the recommended starting dilution for Western blot for this monoclonal antibody, Smad2/3 (A-3): sc-398844 ?

Asked by: TinTin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support 5
Date published: 2017-02-03
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Rated 5 out of 5 by from Good antibodySmad2/3 has been working well for bladder carcinoma cells in our paper.
Date published: 2022-10-25
Rated 4 out of 5 by from very gooda very strong band appeared at expected molecular weight, for human myometrium
Date published: 2019-02-10
Rated 5 out of 5 by from wonderful antibody1:200 diluted for wb of rat protein,the band is quite strong
Date published: 2018-04-11
Rated 5 out of 5 by from 效果很好蛋白条带清洗，背景干净，稀释比例1:300就可以做出来，强烈推荐大家购买santacuz 抗体，，，
Date published: 2017-12-21
Rated 5 out of 5 by from good antibdyThis antibody is good, i got good results! It is a wonderful expriment!
Date published: 2017-10-10
Rated 5 out of 5 by from strong signalThis antibody was used at a dilution of 1:500 , and an excellent WB signal was obtained,specific band.
Date published: 2017-05-13
Rated 5 out of 5 by from Strong Smad 2/3Great detection in WB, IHC(P), and IF for Smad 2/3 detection.
Date published: 2017-02-07
Rated 5 out of 5 by from It woks well for WBI use it at a dilution of 1:5000 in TBS-Tween for overnight and is enough for an excellent wb signal.
Date published: 2017-01-20
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Smad2/3 抗体 (A-3) is rated 4.8 out of 5 by 13.
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