Date published: 2025-9-7
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SIRT1 Antibody (B-7) - Western Blotting - Image 379043
SIRT1 抗体 (B-7): sc-74465. NTERA-2 cl. D1 (A), U-698-M (B), MEG-01 (C), ALL-SIL (D), TF-1 (E) 和 AN3 CA (F) 全细胞裂解液中 SIRT1 表达的 Western 印迹分析.
SIRT1 抗体 (B-7): sc-74465. NTERA-2 cl. D1 (A), U-698-M (B), MEG-01 (C), ALL-SIL (D), TF-1 (E) 和 AN3 CA (F) 全细胞裂解液中 SIRT1 表达的 Western 印迹分析.

SIRT1 抗体 (B-7): sc-74465

4.1(35)
寫評論提問

说明书
  • SIRT1 抗体 B-7 是小鼠单克隆 IgG1 κ，SIRT1抗体, 在175篇文献中引用，规格为200 µg/ml
  • 免疫human物种的SIRT1的氨基酸448-747
  • SIRT1 抗体 (B-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 SIRT1
  • 抗SIRT1抗体（B-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SIRT1 (B-7): sc-74465的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 SIRT1 抗体 (B-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SIRT1 抗体 (B-7) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 SIRT 抗体 包括 SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT7和SIRT6

SIRT1抗体（B-7）是一种小鼠单克隆IgG1 kappa轻链抗体，用于识别人类SIRT1的氨基酸448-747。SIRT1，也称为SIR2、SIR2L1和SIR2alpha，是sirtuin家族的重要成员，是一种NAD⁺依赖性脱乙酰酶，在调节各种细胞过程（包括转录调控、DNA损伤修复、细胞周期进展和衰老）中发挥着关键作用。通过脱乙酰化关键底物（如肿瘤抑制蛋白p53），SIRT1调节了应激反应、代谢控制和长寿所涉及的重要途径，这使得SIRT1成为研究与年龄相关的疾病、癌症和代谢紊乱的重要靶标。抗SIRT1抗体（B-7）具有广泛的物种反应性，可有效检测小鼠、大鼠和人类样本中的SIRT1，适用于多种应用，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）。SIRT1 (B-7) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物，为不同的实验设置提供了多种选择。SIRT1 单克隆抗体（B-7）能够详细研究 SIRT1 在细胞生理学和病理学中的多方面作用，是研究人员阐明关键生物学过程和疾病状态机制的不可或缺的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

SIRT1 抗体 (B-7)

sc-74465
200 µg/ml
RMB2377.00

SIRT1 (B-7): m-IgG Fc BP-HRP 套装

sc-527022
200 µg Ab; 10 µg BP
RMB2662.00

SIRT1 (B-7): m-IgG1 BP-HRP 套装

sc-532395
200 µg Ab; 20 µg BP
RMB2662.00

SIRT1 抗体 (B-7) AC

sc-74465 AC
500 µg/ml, 25% agarose
RMB3129.00

SIRT1 抗体 (B-7) HRP

sc-74465 HRP
200 µg/ml
RMB2377.00

SIRT1 抗体 (B-7) FITC

sc-74465 FITC
200 µg/ml
RMB2482.00

SIRT1 抗体 (B-7) PE

sc-74465 PE
200 µg/ml
RMB2580.00

SIRT1 抗体 (B-7) Alexa Fluor® 488

sc-74465 AF488
200 µg/ml
RMB2685.00

SIRT1 抗体 (B-7) Alexa Fluor® 546

sc-74465 AF546
200 µg/ml
RMB2685.00

SIRT1 抗体 (B-7) Alexa Fluor® 594

sc-74465 AF594
200 µg/ml
RMB2685.00

SIRT1 抗体 (B-7) Alexa Fluor® 647

sc-74465 AF647
200 µg/ml
RMB2685.00

SIRT1 抗体 (B-7) Alexa Fluor® 680

sc-74465 AF680
200 µg/ml
RMB2685.00

SIRT1 抗体 (B-7) Alexa Fluor® 790

sc-74465 AF790
200 µg/ml
RMB2685.00

could we get a sample of SIRT1 antibody to try in our diabetic kidney homogenates?

Asked by: mjrane01
Thank you for your question. Please contact Customer Care so we can arrange shipping of antibody samples with you.
Answered by: Tech Support Europe
Date published: 2020-06-15

If use this antibody, should be use membrane size 0.2 or 0.4 um for transfers protein?

Asked by: cera care
Thank you for your question. We recommend using a PVDF membrane with pore size of 0.45 µm. We also provide this product, UltraCruz® PVDF Transfer membrane: sc-3723.
Answered by: Technical Support
Date published: 2019-02-13

Hi we are doing western blotting with sirt1 antibody conjugate and we don't have any results. we transfer proteins in wet(14 V, over night) or semidry system(25V,1h). We block PVDFor nitrocellulose paper with 3%milk. please help me

Asked by: amkoo
Thank you for your question. As this is a rather complex issue please contact our technical service for assistance.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29
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Rated 4 out of 5 by from Excelente en mamíferoEste anticuerpo funciono a la perfección en mamífero, pero quería determinar si funcionaba con una levadura (Rhodotorula mucilaginosa), pero no fue tan especifíco, me salieron varias bandas. Por eso no lo compre.
Date published: 2024-04-01
Rated 5 out of 5 by from SIRT-1 AntibodyThe Sirt-1 antibody was used in the publication for western blotting and IHC and quality of both turned out to be fabulous. Even with very little use of the antibody we were able to get desired results. The antibody showed good result with both cancerous cell lines and rat and mice tissues samples.
Date published: 2023-12-12
Rated 4 out of 5 by from Good antibody for WBStrong WB signal obtained at 1:1000 dilution in HUVECs cells
Date published: 2023-03-08
Rated 4 out of 5 by from Good antibodyMultiple bands were detected but still expected size band was detected
Date published: 2021-12-03
Rated 5 out of 5 by from very good antibodythe antibody works very well and I recommend it for western blot
Date published: 2021-11-07
Rated 5 out of 5 by from SIRT1 (B-7) antibody tested in western blotThis antibody worked very well in western blot, with standard conditions (25 mg protein, 5% non-fat milk blocking solution, HRP-conjugated anti-mouse antibody)
Date published: 2021-07-26
Rated 3 out of 5 by from satisfactorythis product is works well for western blot analysis
Date published: 2021-02-15
Rated 1 out of 5 by from non funzionaHo comprato un mese fa questo anticorpo e non funziona
Date published: 2020-05-29
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SIRT1 抗体 (B-7) is rated 4.1 out of 5 by 35.
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