Date published: 2025-9-7

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Sigma Receptor 抗体 (B-5): sc-137075

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说明书
  • Sigma Receptor 抗体 B-5 是小鼠单克隆 IgG1 κ,Sigma Receptor抗体, 在78篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Sigma Receptor的内部区域内的氨基酸136-169之间
  • Sigma Receptor 抗体 (B-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Sigma Receptor; 也和以下物种反应,包括: 和 canine, bovine and porcine
  • 抗Sigma Receptor抗体(B-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Sigma Receptor (B-5): sc-137075免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Sigma Receptor 抗体 (B-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Sigma Receptor 抗体 (B-5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Sigma受体抗体(B-5)是一种IgG1 κ小鼠单克隆Sigma受体抗体(也称为SIGMAR1抗体或Sigma1受体抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Sigma受体蛋白。Sigma受体抗体(B-5)既可作为非偶联的抗Sigma受体抗体形式提供,也可作为多种偶联形式的抗Sigma受体抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Sigma受体,也称为阿片类受体,sigma 1(Oprs 1),起着可卡因的作用。在细胞水平上,Sigma受体激动剂调节细胞内调节系统,影响阿片类药物的镇痛活性。例如,Sigma受体的激活是在早期效应r钙动员和细胞外钙流入、NMDA介导的反应和乙酰胆碱释放过程中诱导的。此外,Sigma受体激动剂改变单胺能系统。在行为水平上,Sigma受体参与学习和记忆过程、对应激的反应、抑郁、神经保护和药物依赖。孕烯醇酮、脱氢表雄酮及其硫酸酯表现为Sigma受体激动剂,而孕酮是一种有效的拮抗剂。Sigma受体在内分泌、免疫和其他外周器官系统中表达,并在多种人类肿瘤中表达。Sigma受体负责某些精神疾病的发病机制,并可能涉及中枢神经系统的几种疾病。阿片类镇痛作用受多种因素影响,包括Sigma受体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Sigma Receptor 抗体 (B-5) 参考文献:

  1. 大鼠 sigma1 受体的分子克隆和药理学特征。  |  Mei, J. and Pasternak, GW. 2001. Biochem Pharmacol. 62: 349-55. PMID: 11434908
  2. 神经活性类固醇与 sigma1 受体功能之间的相互作用:行为后果与治疗机会。  |  Maurice, T., et al. 2001. Brain Res Brain Res Rev. 37: 116-32. PMID: 11744080
  3. 小鼠 Sigma1 受体对阿片类镇痛的调节作用  |  Mei, J. and Pasternak, GW. 2002. J Pharmacol Exp Ther. 300: 1070-4. PMID: 11861817
  4. 一种推定的sigma1受体拮抗剂NE-100可减轻氯胺酮对大鼠的辨别刺激效应。  |  Narita, M., et al. 2001. Addict Biol. 6: 373-376. PMID: 11900615
  5. 西格玛受体:从分子到人。  |  Ferris, CD., et al. 1991. J Neurochem. 57: 729-37. PMID: 1650393
  6. 西格玛受体。神经, 内分泌和免疫系统之间的潜在联系。  |  Su, TP. 1991. Eur J Biochem. 200: 633-42. PMID: 1655424
  7. 西格玛受体:生物学和功能。  |  Walker, JM., et al. 1990. Pharmacol Rev. 42: 355-402. PMID: 1964225
  8. Sigma受体配体在癌症诊断和治疗中的潜在应用。  |  van Waarde, A., et al. 2015. Biochim Biophys Acta. 1848: 2703-14. PMID: 25173780
  9. 具有抗增殖和抗癌活性的Sigma受体(σR)配体。  |  Georgiadis, MO., et al. 2017. Molecules. 22: PMID: 28841173
  10. Sigma Receptor Ligands Are Potent Antiprion Compounds that Act Independently of Sigma Receptor Binding.  |  Mercer, RCC., et al. 2024. ACS Chem Neurosci. 15: 2265-2282. PMID: 38743607
  11. 人类 1 型西格玛受体(hSigmaR1)的克隆和功能表达。  |  Kekuda, R., et al. 1996. Biochem Biophys Res Commun. 229: 553-8. PMID: 8954936
  12. 人类 1 型 sigma 受体基因的外显子内含子结构, 启动子区域分析和染色体定位。  |  Prasad, PD., et al. 1998. J Neurochem. 70: 443-51. PMID: 9453537

订购信息

产品名称产品编号规格价格数量收藏夹

Sigma Receptor 抗体 (B-5)

sc-137075
200 µg/ml
RMB2377.00

Sigma Receptor (B-5): m-IgG Fc BP-HRP 套装

sc-528853
200 µg Ab; 10 µg BP
RMB2662.00

Sigma Receptor (B-5): m-IgGκ BP-HRP 套装

sc-521416
200 µg Ab, 40 µg BP
RMB2662.00

Sigma Receptor (B-5): m-IgG1 BP-HRP 套装

sc-543169
200 µg Ab; 20 µg BP
RMB2662.00

Sigma Receptor 抗体 (B-5) AC

sc-137075 AC
500 µg/ml, 25% agarose
RMB3129.00

Sigma Receptor 抗体 (B-5) HRP

sc-137075 HRP
200 µg/ml
RMB2377.00

Sigma Receptor 抗体 (B-5) FITC

sc-137075 FITC
200 µg/ml
RMB2482.00

Sigma Receptor 抗体 (B-5) PE

sc-137075 PE
200 µg/ml
RMB2580.00

Sigma Receptor 抗体 (B-5) Alexa Fluor® 488

sc-137075 AF488
200 µg/ml
RMB2685.00

Sigma Receptor 抗体 (B-5) Alexa Fluor® 546

sc-137075 AF546
200 µg/ml
RMB2685.00

Sigma Receptor 抗体 (B-5) Alexa Fluor® 594

sc-137075 AF594
200 µg/ml
RMB2685.00

Sigma Receptor 抗体 (B-5) Alexa Fluor® 647

sc-137075 AF647
200 µg/ml
RMB2685.00

Sigma Receptor 抗体 (B-5) Alexa Fluor® 680

sc-137075 AF680
200 µg/ml
RMB2685.00

Sigma Receptor 抗体 (B-5) Alexa Fluor® 790

sc-137075 AF790
200 µg/ml
RMB2685.00

Sigma Receptor (B-5) 中和勝肽

sc-137075 P
100 µg/0.5 ml
RMB511.00

Hello, I am looking to use immunofluorescence to view this protein in cancer cells. Is there a recommended permeabilization technique for this membrane embedded receptor (saponin/triton x100 etc)?

Asked by: reo1
Thank you for your question. We have not tested this mouse monoclonal, Sigma Receptor Antibody (B-5): sc-137075, by immunofluorescence in cancer cells and have not determined the optimal permeablization technique for this application. We have tested the antibody in formalin-fixed paraffin-embedded tissue sections and recommend using sodium citrate buffer (pH 6) and heat treatment for antigen retrieval. We would recommend consulting some of the product citations on our website to see how other researchers have used this antibody for IF, e.g., PMID: 24803543.
Answered by: Technical Support
Date published: 2024-10-17

I want to perform a double stain on my mouse tissues. How can I do this with a mouse monoclonal antibody?

Asked by: Cweed
We recommend using conjugated primary antibodies. This product, sc-137075, is available directly conjugated to FITC, AF488, and AF 647.
Answered by: TechService7
Date published: 2024-09-29
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Rated 5 out of 5 by from HjjHjjhkjggh uuuiiiiiiiiiiiiiiiiiii huityiytyuiiiuyggh
Date published: 2024-09-02
Rated 5 out of 5 by from EpicoCompré este producto en estos meses y me encantó..
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Rated 5 out of 5 by from BogosI love what i bought it brins me joy and i use ot evry day
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Rated 5 out of 5 by from SIGMAR1 in FFPWS of adult mouse lungExcellent positivity in macrophages, after Citric acid HIER
Date published: 2024-05-18
Rated 5 out of 5 by from Perfect in Western-blotWe tried this antibody on western-blot with subcellular fractions. It gave a single sharp band at the expected size.
Date published: 2020-10-19
Rated 5 out of 5 by from Great antibody for WesternsStrong, clean band at 1:200 dilution in multiple cancer cell lines. One of my most reliable antibodies.
Date published: 2019-06-18
Rated 5 out of 5 by from Great antibodyUsed this antibody on mouse and human tissues - works very well.
Date published: 2018-05-25
Rated 5 out of 5 by from Effective in C2C12 lysatesWe used this antibody in western blots of C2C12 lysates. At a 1:1000 dilution, the antibody gave faint but visible bands at the correct M.W. of 30 kDa.
Date published: 2017-12-05
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Sigma Receptor 抗体 (B-5) is rated 4.9 out of 5 by 18.
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