Date published: 2025-9-8
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SHP Antibody (H-5) - Immunohistochemistry - Image 285707
SHP 抗体 (H-5): sc-271511. 福尔马林固定, 石蜡包埋的人肝组织的免疫过氧化物酶染色, 显示肝细胞膜染色以及窦状细胞的胞浆和膜染色.
SHP Antibody (H-5) - Immunohistochemistry - Image 285707
SHP 抗体 (H-5): sc-271511. 福尔马林固定, 石蜡包埋的人肝组织的免疫过氧化物酶染色, 显示肝细胞膜染色以及窦状细胞的胞浆和膜染色.
SHP Antibody (H-5) - Western Blotting - Image 133903
SHP 抗体 (H-5): sc-271511. 非转染: sc-117752 (A) 和人 SHP 转染: sc-114141 (B) 293T 全细胞裂解液中 SHP 表达的 Western 印迹分析.
SHP Antibody (H-5) - Western Blotting - Image 370465
SHP 抗体 (H-5) HRP: sc-271511 HRP. 非转染: sc-117752 (A) 和人 SHP 转染: sc-114141 (B) 293T 全细胞裂解液中 SHP 表达的直接 western 印迹分析.
SHP 抗体 (H-5): sc-271511. 福尔马林固定, 石蜡包埋的人肝组织的免疫过氧化物酶染色, 显示肝细胞膜染色以及窦状细胞的胞浆和膜染色.

SHP 抗体 (H-5): sc-271511

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寫評論提問

说明书
  • SHP 抗体 H-5 是小鼠单克隆 IgG2a κ，SHP抗体, 在13篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-160位于human物种的SHP的N-terminus
  • SHP 抗体 (H-5) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 SHP
  • 抗SHP抗体（H-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SHP (H-5): sc-271511的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 SHP 抗体 (H-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SHP 抗体 (H-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

SHP抗体（H-5）是一种IgG2a κ小鼠单克隆SHP抗体（也称为核受体亚家族0组B成员2抗体、小异二聚体伴侣（SHP）抗体、孤儿核受体SHP抗体、核受体SHP抗体或SHP1抗体），通过WB、IP、IF、IHC(P)和ELISA检测人源SHP蛋白。SHP抗体（H-5）既有非共轭的抗SHP抗体形式，也有多种共轭形式的抗SHP抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。SHP（short heterodimer partner，小异二聚体伴侣）是一种孤儿核受体，包含在其他核受体中发现的二聚化和配体结合域，但缺乏保守的DNA结合域。SHP特异性地表达于肝脏和其他组织，包括胎儿肝脏和肾上腺，以及成人的脾脏和小肠。此外，SHP在小鼠巨噬细胞系RAW 264.7中高表达，但受到oxLDL和13-HODE的抑制，后者是PPARγ的配体。SHP与核受体相互作用，包括甲状腺激素受体、维甲酸受体（RAR和RXR）以及雌激素受体（ERα和ERβ）。SHP至少通过三种机制作为这些受体的负调节因子：通过二聚化抑制DNA结合，通过竞争直接拮抗共激活剂的功能，以及可能通过招募假定的共抑制因子进行反式抑制。在经oxLDL处理的静息巨噬细胞中，SHP作为NF-κB的转录共激活因子，这表明SHP是NF-κB转录活性调节中的一个调节成分。最后，在胆汁淤积条件下，为了保护肝细胞免受胆汁酸介导的损伤，胆汁酸激活的FXR通过诱导SHP来控制肝胆汁酸转运蛋白NTCP的负反馈调节。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SHP 抗体 (H-5) 参考文献:

  1. 孤儿核受体 SHP 利用保守的 LXXLL 相关基团与配体激活的雌激素受体相互作用。  |  Johansson, L., et al. 2000. Mol Cell Biol. 20: 1124-33. PMID: 10648597
  2. 短异构体伙伴（SHP）孤儿核受体抑制芳基烃受体（AHR）/AHR 核转运体（ARNT）的转录活性。  |  Klinge, CM., et al. 2001. Arch Biochem Biophys. 390: 64-70. PMID: 11368516
  3. 孤儿核受体 shp 介导了胆汁酸对大鼠胆汁酸转运体 ntcp 的抑制。  |  Denson, LA., et al. 2001. Gastroenterology. 121: 140-7. PMID: 11438503
  4. 在氧化低密度脂蛋白处理的巨噬细胞系 RAW 264.7 中，孤儿核受体小杂二聚体伙伴是核因子-kappa b 的新型核心调节因子。  |  Kim, YS., et al. 2001. J Biol Chem. 276: 33736-40. PMID: 11448950
  5. 在人类子宫内膜癌细胞中，他莫昔芬的激动剂活性受到短异构体伙伴孤儿核受体的抑制。  |  Klinge, CM., et al. 2002. Endocrinology. 143: 853-67. PMID: 11861507
  6. 人类前脂肪细胞中短异源二聚体伙伴通过启动子 II 抑制芳香化酶的转录物。  |  Kovacic, A., et al. 2004. Mol Endocrinol. 18: 252-9. PMID: 14593077
  7. 过表达短异源二聚体伙伴可恢复过表达 UCP2 的胰腺β细胞在葡萄糖刺激下受损的胰岛素分泌。  |  Suh, YH., et al. 2004. J Endocrinol. 183: 133-44. PMID: 15525581
  8. 核受体 SHP 在新陈代谢和癌症中的作用。  |  Zhang, Y., et al. 2011. Biochim Biophys Acta. 1812: 893-908. PMID: 20970497
  9. SHP-1调控CD8+ T细胞效应器功能，但由于阶段特异性非冗余功能中继，SHP-1与SHP-2在T细胞衰竭中发挥着微妙的作用。  |  Hou, B., et al. 2024. J Immunol. 212: 397-409. PMID: 38088801
  10. 一种孤儿核荷尔蒙受体，缺乏 DNA 结合结构域，可与其他受体异源二聚。  |  Seol, W., et al. 1996. Science. 272: 1336-9. PMID: 8650544
  11. 孤儿核受体 SHP 基因的结构和表达。  |  Lee, HK., et al. 1998. J Biol Chem. 273: 14398-402. PMID: 9603951
  12. 孤儿受体 SHP（短异二聚体伙伴）对雌激素受体作用的抑制。  |  Seol, W., et al. 1998. Mol Endocrinol. 12: 1551-7. PMID: 9773978

订购信息

产品名称产品编号规格价格数量收藏夹

SHP 抗体 (H-5)

sc-271511
200 µg/ml
RMB2377.00

SHP (H-5): m-IgG Fc BP-HRP 套装

sc-525811
200 µg Ab; 10 µg BP
RMB2662.00

SHP (H-5): m-IgG2a BP-HRP 套装

sc-546423
200 µg Ab; 10 µg BP
RMB2662.00

SHP 抗体 (H-5) AC

sc-271511 AC
500 µg/ml, 25% agarose
RMB3129.00

SHP 抗体 (H-5) HRP

sc-271511 HRP
200 µg/ml
RMB2377.00

SHP 抗体 (H-5) FITC

sc-271511 FITC
200 µg/ml
RMB2482.00

SHP 抗体 (H-5) PE

sc-271511 PE
200 µg/ml
RMB2580.00

SHP 抗体 (H-5) Alexa Fluor® 488

sc-271511 AF488
200 µg/ml
RMB2685.00

SHP 抗体 (H-5) Alexa Fluor® 546

sc-271511 AF546
200 µg/ml
RMB2685.00

SHP 抗体 (H-5) Alexa Fluor® 594

sc-271511 AF594
200 µg/ml
RMB2685.00

SHP 抗体 (H-5) Alexa Fluor® 647

sc-271511 AF647
200 µg/ml
RMB2685.00

SHP 抗体 (H-5) Alexa Fluor® 680

sc-271511 AF680
200 µg/ml
RMB2685.00

SHP 抗体 (H-5) Alexa Fluor® 790

sc-271511 AF790
200 µg/ml
RMB2685.00

Can this antibody detect a truncated SHP which is only 1-52 aa? Has the epitope been mapped? I am looking for one that can detect the truncated mutant. Thanks.

Asked by: MLKK
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-01-26

Is there anti-shp for mouse?

Asked by: Hanwang
Thank you for your question. We currently offer 3 different mouse monoclonal antibodies against SHP, and all 3 are recommended for detection of SHP of human origin. We have not yet confirmed reactivity with mouse or rat SHP for these antibodies. The antibodies are raised against a recombinant protein that does share 77% sequence identity with the mouse SHP protein, so there is a very good chance that they will also be reactive with mouse. For a limited time, we are offering a free 10 µg sample of our mouse monoclonal antibody, SHP (H-5): sc-271511. Please contact our Technical Service Department if you would like to request a sample for testing. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-05-10

Can SHP (H-5): sc-271511 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, SHP (H-5): sc-271511 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from SHP antibodyI detect the cell protein by SHP antibody sample and find the band when 1:100 dilution.
Date published: 2018-01-05
Rated 5 out of 5 by from specific band using mice liver lysate at 1:200very specific bands when using mice liver lysate at 1:200
Date published: 2017-10-12
Rated 5 out of 5 by from worked well for WBStrong, specific band was showed in WB in protein lysates of HepG2 cells.
Date published: 2017-09-17
Rated 4 out of 5 by from WB ResultsI applied for a sample two week ago.My sample is the mouse liver. Incubated the antibody for overnight at 4℃, 1:1000 dilution, and  m-IgGκ BP-HRP  incubated for 1 hour at RT , 1:1000 dilution.The results are shown in the figure.
Date published: 2017-09-16
Rated 5 out of 5 by from Service is goodService is good. However, it may be not applicable to experiment about fish.
Date published: 2017-05-18
Rated 5 out of 5 by from great featuresWB on mice Liver sample，diluted with 1：500,which shows good quality and got bands very clearly.
Date published: 2017-05-08
Rated 5 out of 5 by from SHP(H-5) recognize recombinant SHPThe SHP(H-5) antibody was used at 1:500 dilution in WB. It successfully detected significant bands for recombinant mouse and human SHP proteins expressed in HEK293T cells.
Date published: 2017-01-13
Rated 5 out of 5 by from Exceptional immunoperoxidase membrane stainingExceptional immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human liver tissue. -SCBT QC
Date published: 2015-02-05
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SHP 抗体 (H-5) is rated 4.8 out of 5 by 9.
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