Date published: 2025-9-7
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SHIP-1 Antibody (P1C1) - Western Blotting - Image 379367
SHIP-1 抗体 (P1C1): sc-8425. THP-1 (A), U-698-M (B), HEL 92. 1. 7 (C), Daudi (D) 和 M1 (E) 全细胞裂解液中 SHIP-1 表达的 Western 印迹分析.
SHIP-1 抗体 (P1C1): sc-8425. THP-1 (A), U-698-M (B), HEL 92. 1. 7 (C), Daudi (D) 和 M1 (E) 全细胞裂解液中 SHIP-1 表达的 Western 印迹分析.

SHIP-1 抗体 (P1C1): sc-8425

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寫評論提問

说明书
  • SHIP-1 抗体 P1C1 是小鼠单克隆 IgG1 κ，SHIP-1抗体, 在141篇文献中引用，规格为200 µg/ml
  • 免疫mouse来源SHIP-1位于C-terminus 的866-1020氨基酸
  • SHIP-1 抗体 (P1C1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 SHIP-1 p145
  • 抗SHIP-1抗体（P1C1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SHIP-1 (P1C1): sc-8425的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 SHIP-1 抗体 (P1C1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SHIP-1 抗体 (P1C1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 SHIP 抗体 包括 SHIP-2

SHIP-1抗体（P1C1）是一种小鼠单克隆IgG1 kappa轻链抗体，用于检测小鼠、大鼠和人类样本中的SHIP-1（也称为INPP5D）。SHIP-1是一种含有SH2的肌醇5-磷酸酶，是PI3K信号传导通路的关键调节因子。SHIP-1通过将磷脂酰肌醇-3,4,5-三磷酸（PIP3）去磷酸化为磷脂酰肌醇-4,5-二磷酸（PIP2），减弱免疫细胞激活、增殖和存活的信号级联。这种负调控对于维持免疫平衡和保护免受过度激活至关重要，过度激活会导致炎症或自身免疫性疾病。SHIP-1主要在造血细胞中表达，参与造血、肥大细胞脱颗粒和B细胞受体信号传导等关键过程。SHIP-1单克隆抗体（P1C1）靶向高度保守的C端表位，包括866-1020个氨基酸，可用于包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）在内的多种应用。SHIP-1 单克隆抗体（P1C1）有非结合型和结合型两种形式，如辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和 Alexa Fluor® 变体。SHIP-1 单克隆抗体（P1C1）推荐用于信号转导、免疫细胞调节和造血发育研究。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SHIP-1 抗体 (P1C1) 参考文献:

  1. 集落刺激因子 1 在细胞周期的 G1 阶段调节新型细胞周期蛋白。  |  Matsushime, H., et al. 1991. Cell. 65: 701-13. PMID: 1827757
  2. c-fms 原癌基因产物与单核吞噬细胞生长因子 CSF-1 的受体有关。  |  Sherr, CJ., et al. 1985. Cell. 41: 665-76. PMID: 2408759
  3. c-fms 原癌基因（CSF-1 受体）的转化潜能。  |  Roussel, MF., et al. Nature. 325: 549-52. PMID: 3027579
  4. 人类 c-fms 癌基因的染色体定位。  |  Groffen, J., et al. 1983. Nucleic Acids Res. 11: 6331-9. PMID: 6684766
  5. 由多种细胞因子诱导与 Shc 结合的 145 kDa 蛋白是一种三磷酸肌醇和 3,4,5-三磷酸肌醇 5-磷酸酶。  |  Damen, JE., et al. 1996. Proc Natl Acad Sci U S A. 93: 1689-93. PMID: 8643691
  6. p150Ship，一种具有肌醇多磷酸-5-磷酸酶活性的信号转导分子。  |  Lioubin, MN., et al. 1996. Genes Dev. 10: 1084-95. PMID: 8654924

订购信息

产品名称产品编号规格价格数量收藏夹

SHIP-1 抗体 (P1C1)

sc-8425
200 µg/ml
RMB2377.00

SHIP-1 (P1C1): m-IgG Fc BP-HRP 套装

sc-528229
200 µg Ab; 10 µg BP
RMB2662.00

SHIP-1 (P1C1): m-IgGκ BP-HRP 套装

sc-520555
200 µg Ab, 40 µg BP
RMB2662.00

SHIP-1 (P1C1): m-IgG1 BP-HRP 套装

sc-542828
200 µg Ab; 20 µg BP
RMB2662.00

SHIP-1 抗体 (P1C1) AC

sc-8425 AC
500 µg/ml, 25% agarose
RMB3129.00

SHIP-1 抗体 (P1C1) HRP

sc-8425 HRP
200 µg/ml
RMB2377.00

SHIP-1 抗体 (P1C1) FITC

sc-8425 FITC
200 µg/ml
RMB2482.00

SHIP-1 抗体 (P1C1) PE

sc-8425 PE
200 µg/ml
RMB2580.00

SHIP-1 抗体 (P1C1) Alexa Fluor® 488

sc-8425 AF488
200 µg/ml
RMB2685.00

SHIP-1 抗体 (P1C1) Alexa Fluor® 546

sc-8425 AF546
200 µg/ml
RMB2685.00

SHIP-1 抗体 (P1C1) Alexa Fluor® 594

sc-8425 AF594
200 µg/ml
RMB2685.00

SHIP-1 抗体 (P1C1) Alexa Fluor® 647

sc-8425 AF647
200 µg/ml
RMB2685.00

SHIP-1 抗体 (P1C1) Alexa Fluor® 680

sc-8425 AF680
200 µg/ml
RMB2685.00

SHIP-1 抗体 (P1C1) Alexa Fluor® 790

sc-8425 AF790
200 µg/ml
RMB2685.00

Is there information on the domain/residues this antibody recognizes? I suppose this is specific to the SH2 domain or phosphatase domain?

Asked by: pwandy
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-04-07

想问下这个抗体是看ship1磷酸化的吗？

Asked by: essssss
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Is a blocking buffer for mouse Fc recommended when reacting with mouse tissue (specifically, mouse brain)?

Asked by: dzaj
Thank you for your question. You can block mouse Fc receptors by pre-incubating with normal mouse IgG1: sc-3877 when using sc-8425 or any other IgG1 primary antibody.
Answered by: Tech Support Europe
Date published: 2020-12-14

Wir sind eine Protein Produktions-Core Facility und sollen Ship1 herstellen. Wäre es möglich eine Gratisprobe von SHIP-1 (P1C1): sc-8425 zu bekommen? Können an Agarose gekoppelte anti-Ship-1 Antikörper auch zur Reinigung der Ship benutzt werden?

Asked by: Anonymous
Vielen Dank für Ihre Anfrage. Bitte kontaktieren Sie unseren technischen Support um ein 10 µg gratis Sample vom SHIP-1 (P1C1): sc-8425 zu erhalten.
Answered by: Tech Support Europe
Date published: 2020-12-08

Can this mouse monoclonal antibody be used in IF or IHC on mouse tissue without background or non-specific staining issues?

Asked by: AbPolly
Thank you for your question. Yes, it can be used successfully for this application. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody SHIP-1 (P1C1) is currently available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Support 13
Date published: 2020-09-21
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Rated 2 out of 5 by from Correct band only with specific conditionRequire a transfer overnight and detection with prime product on primary human macrophages.
Date published: 2017-10-17
Rated 5 out of 5 by from anti-SHIP1 for WBThis antibody works very well for ship1 detection by immunoblotting on B cell line.
Date published: 2017-08-31
Rated 5 out of 5 by from anti-SHIP-1 is effectiveI diluted anti SHIP as 1 :200, and it can be use as more than 3 times, all of the results is perfect.
Date published: 2017-06-30
Rated 5 out of 5 by from Excellent!!Strong western blot data;nice signal with low background
Date published: 2017-01-03
Rated 5 out of 5 by from The only SHIP-1 antibody that you will need.The best choice you can make when studying SHIP-1. This antibody performs in all standard applications and is reactive with mouse, rat, and human. It has good data and over 75 citations to support it.
Date published: 2016-12-29
Rated 5 out of 5 by from SHIP1 antibodyThis has worked very well in WB. It is predictable and reliable.
Date published: 2016-10-19
Rated 5 out of 5 by from Have used this for yearsHave used this for years. Very good for WB and IP. Very clean results.
Date published: 2015-10-09
Rated 3 out of 5 by from Working okayWorking okay, about the same as other SHIP-1 antibodies from Santa Cruz.
Date published: 2015-06-16
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SHIP-1 抗体 (P1C1) is rated 4.3 out of 5 by 15.
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