Date published: 2025-9-6
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Shc Antibody (PG-797) - Western Blotting - Image 354858
Shc 抗体 (PG-797): sc-967. A549 (A), HEK293 (B), NIH/3T3 (C), NRK (D) 和 A-10 (E) 全细胞裂解液中 Shc 表达的 Western 印迹分析.
Shc 抗体 (PG-797): sc-967. A549 (A), HEK293 (B), NIH/3T3 (C), NRK (D) 和 A-10 (E) 全细胞裂解液中 Shc 表达的 Western 印迹分析.

Shc 抗体 (PG-797): sc-967

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说明书
  • Shc抗体(PG-797)是小鼠单克隆IgG1 κ, 在83篇文献中引用，规格为200 µg/ml
  • 针对氨基酸残基476-583的Shc的human起源
  • 抗-Shc 抗体 (PG-797) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 Shc p66, p52 and p46
  • 抗Shc抗体（PG-797）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Shc (PG-797): sc-967的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 是 Shc 抗体 (PG-797) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Shc 抗体 (PG-797) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
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研究信息

関連項目

Shc抗体（PG-797）是一种鼠单克隆IgG1 kappa轻链抗体，可通过应用诸如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）等方法检测小鼠、大鼠和人类样本中的Shc。Shc 单克隆抗体（PG-797）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 结合物。Shc在细胞信号传导通路中发挥着关键作用，特别是在生长因子受体激活的情况下。Shc是一种连接受体酪氨酸激酶和下游信号分子的适配蛋白，有助于信号的传递，从而引发细胞反应，如增殖和分化。已知Shc与多种关键蛋白相互作用，包括GRB2和Sos，它们对于激活Ras信号通路至关重要，而Ras信号通路是细胞生长和存活的关键通路。Shc能够与这些蛋白质形成复合物，这凸显了它在胰岛素信号传导和其他有丝分裂反应中的重要性，也突出了它在复杂的细胞通讯网络中扮演的关键角色。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Shc 抗体 (PG-797) 参考文献:

  1. Shc 蛋白被 v-Src 和 v-Fps 蛋白-酪氨酸激酶磷酸化并受其调节。  |  McGlade, J., et al. 1992. Proc Natl Acad Sci U S A. 89: 8869-73. PMID: 1409579
  2. 一种具有 SH2 结构域的新型转化蛋白（SHC）与有丝分裂信号转导有关。  |  Pelicci, G., et al. 1992. Cell. 70: 93-104. PMID: 1623525
  3. 转化和有丝分裂酪氨酸激酶对 GAP 和 GAP 相关蛋白的磷酸化作用。  |  Ellis, C., et al. 1990. Nature. 343: 377-81. PMID: 1689011
  4. 血小板衍生生长因子（PDGF）依赖磷脂酶 C-gamma 与 PDGF 受体信号传导复合物的缔合作用。  |  Morrison, DK., et al. 1990. Mol Cell Biol. 10: 2359-66. PMID: 1691440
  5. SH2 和 SH3 结构域：控制细胞质信号蛋白相互作用的元件。  |  Koch, CA., et al. 1991. Science. 252: 668-74. PMID: 1708916
  6. 癌基因和信号转导  |  Cantley, LC., et al. 1991. Cell. 64: 281-302. PMID: 1846320
  7. 具有酪氨酸激酶活性的受体的信号转导。  |  Ullrich, A. and Schlessinger, J. 1990. Cell. 61: 203-12. PMID: 2158859
  8. Shc与细胞锚定和转移的机械传导。  |  Terada, LS. 2019. Small GTPases. 10: 64-71. PMID: 28632027
  9. 阿尔茨海默病中的神经元 Shc 适应子  |  Triaca, V., et al. 2018. Aging (Albany NY). 10: 5-6. PMID: 29362290
  10. Shc与钙网织蛋白介导的酒精性肝炎无菌性炎症有关  |  Li, Y., et al. 2023. Cell Mol Gastroenterol Hepatol. 15: 197-211. PMID: 36122677
  11. p66Shc 氧化还原蛋白与新出现的糖尿病并发症。  |  Biondi, G., et al. 2023. Int J Mol Sci. 25: PMID: 38203279
  12. T 细胞激活时 Shc 与 T 细胞受体 zeta 链的相互作用。  |  Ravichandran, KS., et al. 1993. Science. 262: 902-5. PMID: 8235613

订购信息

产品名称产品编号规格价格数量收藏夹

Shc 抗体 (PG-797)

sc-967
200 µg/ml
RMB2377.00

Shc (PG-797): m-IgG1 BP-HRP 套装

sc-531625
200 µg Ab; 20 µg BP
RMB2662.00

Shc 抗体 (PG-797) AC

sc-967 AC
500 µg/ml, 25% agarose
RMB3129.00

Shc 抗体 (PG-797) HRP

sc-967 HRP
200 µg/ml
RMB2377.00

Shc 抗体 (PG-797) FITC

sc-967 FITC
200 µg/ml
RMB2482.00

Shc 抗体 (PG-797) PE

sc-967 PE
200 µg/ml
RMB2580.00

Shc 抗体 (PG-797) Alexa Fluor® 488

sc-967 AF488
200 µg/ml
RMB2685.00

Shc 抗体 (PG-797) Alexa Fluor® 546

sc-967 AF546
200 µg/ml
RMB2685.00

Shc 抗体 (PG-797) Alexa Fluor® 594

sc-967 AF594
200 µg/ml
RMB2685.00

Shc 抗体 (PG-797) Alexa Fluor® 647

sc-967 AF647
200 µg/ml
RMB2685.00

Shc 抗体 (PG-797) Alexa Fluor® 680

sc-967 AF680
200 µg/ml
RMB2685.00

Shc 抗体 (PG-797) Alexa Fluor® 790

sc-967 AF790
200 µg/ml
RMB2685.00

Can I use Shc (PG-797): sc-967 mouse monoclonal antibody for immunofluorescence staining of mouse tissue?

Asked by: Anonymous
Thank you for your question. Yes, this mouse monoclonal antibody can be used for immunofluorescence staining (IF) of mouse tissue. We recommend you include a secondary antibody control (omit primary antibody) to identify any possible non-specific staining from your anti-mouse IgG secondary antibody alone. Alternatively, please consider using one of the directly conjugated forms of this antibody for IF.
Answered by: Technical Support
Date published: 2019-05-13

Can I use Shc (PG-797): sc-967 mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: cjMara
Yes. We recommend using either a directly conjugated monoclonal antibody. Shc (PG-797): sc-967 monoclonal antibody is available directly conjugated to either HRP, FITC, PE, Alexa Fluor® 488 or Alexa Fluor® 647. Alternatively, we recommend performing indirect staining using our recommended mouse IgG binding protein conjugated to HRP (sc-516102). https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp?requestFrom=search
Answered by: Technical Support 5
Date published: 2017-01-20
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Rated 5 out of 5 by from Great antibody in specificityIt works well in WB at 1:200 and has a good specificity.
Date published: 2017-08-17
Rated 5 out of 5 by from SuperbGreat antibody for both western blot and IHC staining
Date published: 2017-02-05
Rated 5 out of 5 by from Good antibody!I use this antibody in WB with rat protein ,the result is wonderful,recommen with a dilution 1:500
Date published: 2017-01-13
Rated 5 out of 5 by from Popular antibody!Shc Antibody (PG-797) is a well established clone with over 35 product citations, and popular choice for use in a variety of research applications.
Date published: 2016-12-28
Rated 4 out of 5 by from Nice publishable data on Western blot onNice publishable data on Western blot on mouse 32D cells. -SCBT Publication Review
Date published: 2015-06-22
Rated 5 out of 5 by from Good specificity and signal strength in MCF7Good specificity and signal strength in MCF7 whole cell lysate. -SCBT QC
Date published: 2015-05-27
Rated 5 out of 5 by from Produced exceptional intracellular FCM dataProduced exceptional intracellular FCM data for fixed and permeabilized MCF7 cells. -SCBT QC
Date published: 2015-05-20
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic stainingExcellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human small intestine tissue. -SCBT QC
Date published: 2015-04-17
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Shc 抗体 (PG-797) is rated 4.7 out of 5 by 15.
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