Date published: 2025-9-7

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SESN2 抗体 (D-4): sc-393195

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说明书
  • SESN2 抗体 D-4 是小鼠单克隆 IgG1 κ,SESN2抗体, 在7篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列45-106位于human物种的SESN2的N-terminus附近
  • SESN2 抗体 (D-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 SESN2
  • 抗SESN2抗体(D-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SESN2 (D-4): sc-393195免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 是 SESN2 抗体 (D-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与SESN2 抗体 (D-4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

SESN2抗体(D-4)是一种IgG1 κ小鼠单克隆SESN2抗体(也称为SEST2抗体、Sestrin-2抗体、SES2抗体或Hypoxia Induced Gene 95抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的SESN2蛋白。SESN2抗体(D-4)既可以是非偶联的抗-SESN2抗体形式,也可以是多种偶联形式的抗-SESN2抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。SESN2,也称为sestrin 2、HI95、SES2或SEST2,是一种由480个氨基酸组成的蛋白质,属于与PA26相关的sestrin家族蛋白质。SESN2在全身多种组织中表达,被认为参与细胞生长和存活的调节,并可能在介导应激诱导的细胞应答中发挥作用。在氧化应激或DNA损伤后,SESN2的表达会上调,导致细胞毒性和随后的凋亡,暗示SESN2在调节细胞活力中起着重要作用。相反,在乳腺癌细胞中过表达SESN2可以保护细胞免受凋亡,提示SESN2可能在肿瘤进展中发挥作用。因此,SESN2是细胞存活的关键调节因子,其功能根据细胞条件的不同而有所不同。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SESN2 抗体 (D-4) 参考文献:

  1. 鉴定参与细胞活力调控的新型应激反应基因 Hi95。  |  Budanov, AV., et al. 2002. Oncogene. 21: 6017-31. PMID: 12203114
  2. PA26 是人类异位症的候选基因:在人类和小鼠中鉴定出一个新的 PA26 相关基因家族。  |  Peeters, H., et al. 2003. Hum Genet. 112: 573-80. PMID: 12607115
  3. 由 p53 调节的 sestrins(细菌 AhpD 的同源物)再生过氧化物酶。  |  Budanov, AV., et al. 2004. Science. 304: 596-600. PMID: 15105503
  4. 胰岛素家族基因的抑制有助于致癌物质 Ras 诱导的活性氧上调和遗传不稳定性。  |  Kopnin, PB., et al. 2007. Cancer Res. 67: 4671-8. PMID: 17510393
  5. SESN2/雌激素2诱导介导的自噬和异黄酮甙元(ISO)对人类膀胱癌的抑制作用。  |  Liang, Y., et al. 2016. Autophagy. 12: 1229-39. PMID: 27171279
  6. SESN2 通过 ULK1 介导的 Beclin1 磷酸化帮助 Parkin 转位,从而促进有丝分裂。  |  Kumar, A. and Shaha, C. 2018. Sci Rep. 8: 615. PMID: 29330382
  7. 组蛋白去甲基化酶 JMJD3 通过抑制 SESN2 的表达介导多柔比星诱发的心肌病  |  Wang, P., et al. 2020. Front Cell Dev Biol. 8: 548605. PMID: 33117796
  8. SESN2通过AMPK/PGC-1α途径防止变性萎缩中肌纤维由慢到快的转变。  |  Yang, X., et al. 2022. Cell Mol Biol Lett. 27: 66. PMID: 35945510
  9. RUBCN/SESN2 介导的自噬抑制作为糖尿病肾病生物标志物的临床应用。  |  Watany, MM., et al. 2022. Mol Med. 28: 147. PMID: 36476132
  10. Sestrin2 通过 SESN2/AMPK 途径调节有丝分裂,从而保护缺氧性神经损伤。  |  Pan, C., et al. 2023. Front Mol Biosci. 10: 1266243. PMID: 37808523
  11. 西柚酮通过 SESN2-NRF2/ HO-1 通路抑制 NLRP3 相关炎症体,从而抑制肾小球纤维化  |  Xiao, T., et al. 2025. Diabetes Metab J. 49: 34-48. PMID: 39192821
  12. SETDB1靶向SESN2调节肾缺血再灌注损伤中的线粒体损伤和氧化应激。  |  Xia, K., et al. 2024. BMC Biol. 22: 246. PMID: 39443993

订购信息

产品名称产品编号规格价格数量收藏夹

SESN2 抗体 (D-4)

sc-393195
200 µg/ml
RMB2377.00

SESN2 (D-4): m-IgG1 BP-HRP 套装

sc-531756
200 µg Ab; 20 µg BP
RMB2662.00

SESN2 抗体 (D-4) AC

sc-393195 AC
500 µg/ml, 25% agarose
RMB3129.00

SESN2 抗体 (D-4) HRP

sc-393195 HRP
200 µg/ml
RMB2377.00

SESN2 抗体 (D-4) FITC

sc-393195 FITC
200 µg/ml
RMB2482.00

SESN2 抗体 (D-4) PE

sc-393195 PE
200 µg/ml
RMB2580.00

SESN2 抗体 (D-4) Alexa Fluor® 488

sc-393195 AF488
200 µg/ml
RMB2685.00

SESN2 抗体 (D-4) Alexa Fluor® 546

sc-393195 AF546
200 µg/ml
RMB2685.00

SESN2 抗体 (D-4) Alexa Fluor® 594

sc-393195 AF594
200 µg/ml
RMB2685.00

SESN2 抗体 (D-4) Alexa Fluor® 647

sc-393195 AF647
200 µg/ml
RMB2685.00

SESN2 抗体 (D-4) Alexa Fluor® 680

sc-393195 AF680
200 µg/ml
RMB2685.00

SESN2 抗体 (D-4) Alexa Fluor® 790

sc-393195 AF790
200 µg/ml
RMB2685.00

Please give me the reference (published data) on the usage of this AB ( sc-393195) in ELISA. Thanks.

Asked by: Meloman
Thank you for your question. We do not have any publications for ELISA. We have a on year warranty from date of purchase .
Answered by: BlakeJ
Date published: 2023-02-15

I am using SESN2 (D-4): sc-393195 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Very good in WBWB on 10µg of total mouse heart protein extract. Gel 10%, single band detected at 1/1000 and 1/10000 for secondary Ab
Date published: 2017-10-21
Rated 4 out of 5 by from No band detected in U87 cell lysates (WB)Protein expression was not visible for western blot with 25 ug U87 cell lysates at 1:1000 primary antibody dilution.
Date published: 2017-09-10
Rated 5 out of 5 by from works wellworked well with pancreatic cancer cell lines at 1/1000 dilution
Date published: 2017-09-01
Rated 5 out of 5 by from Great monoclonal antibodyNice and strong bands in various samples with low to no background. IHCP stain was robust and specific
Date published: 2017-02-02
Rated 5 out of 5 by from Good signal with WBI confirmed strong and specific signals using Sesn2 WT and KO MEFs
Date published: 2016-09-09
Rated 5 out of 5 by from Produced great immunoperoxidase nuclear andProduced great immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human colon tissue. -SCBT QC
Date published: 2015-03-09
Rated 5 out of 5 by from Great Western Blot data of SESN2 expression in nonGreat Western Blot data of SESN2 expression in non-transfected 293T, human SESN2 transfected 293T and U-87 MG whole cell lysates. -SCBT QC
Date published: 2014-11-03
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SESN2抗体 (D-4) is rated 4.9 out of 5 by 7.
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