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SerpinB8 抗体 (PI-8): sc-101371

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说明书
  • SerpinB8 抗体 PI-8 是小鼠单克隆 IgG1 κ,SerpinB8抗体, 在1篇文献中引用,规格为200 µg/ml
  • 免疫human物种的SerpinB8
  • SerpinB8 抗体 (PI-8) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 SerpinB8
  • 抗SerpinB8抗体(PI-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SerpinB8 (PI-8): sc-101371免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 SerpinB8 抗体 (PI-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SerpinB8 抗体 (PI-8) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

SerpinB8抗体(PI-8)是一种IgG1 κ小鼠单克隆SerpinB8抗体(也称为CAP2抗体),可通过WB、IP、IF、IHC(P)和ELISA检测人源SerpinB8蛋白。SerpinB8抗体(PI-8)既可以是非偶联的抗SerpinB8抗体形式,也可以是多种偶联形式的抗SerpinB8抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。丝氨酸蛋白酶抑制剂(serpins)构成了一个具有多种功能的蛋白质超家族,包括控制血液凝固、补体激活、程序性细胞死亡和组织发育等。SerpinB8(丝氨酸蛋白酶抑制剂,分支B(卵清蛋白),成员8),也称为P18或CAP2,是一种由374个氨基酸组成的蛋白质,定位于细胞质中,属于丝氨酸蛋白酶抑制剂家族。SerpinB8在肺、肝、心和骨骼肌中高表达,以多种选择性剪接异构体形式存在,被认为在血小板聚集和血小板调节的病理生理反应中发挥作用。编码SerpinB8的基因定位于人类18号染色体上的一组Serpin基因中,该基因簇包含300多个蛋白质编码基因,含有近7600万个碱基。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SerpinB8 抗体 (PI-8) 参考文献:

  1. 人类细胞内丝氨酸蛋白酶抑制剂 8 在人体组织中的分布。  |  Strik, MC., et al. 2002. J Histochem Cytochem. 50: 1443-54. PMID: 12417609
  2. 血清素对凝血系统的控制。在糖胺聚糖的帮助下度过难关。  |  Pike, RN., et al. 2005. FEBS J. 272: 4842-51. PMID: 16176258
  3. 丝蛋白蛋白酶抑制剂 8:一种从人体血小板中释放的内源性呋喃抑制剂。  |  Leblond, J., et al. 2006. Thromb Haemost. 95: 243-52. PMID: 16493485
  4. α-1-抗胰蛋白酶三维结构对丝氨酸结构和功能的影响。  |  Huber, R. and Carrell, RW. 1989. Biochemistry. 28: 8951-66. PMID: 2690952
  5. 卵清蛋白家族丝氨酸蛋白酶抑制剂两个新成员的分子克隆, 表达和部分表征。  |  Sprecher, CA., et al. 1995. J Biol Chem. 270: 29854-61. PMID: 8530382
  6. 细胞质抗蛋白酶 2 (PI8) 和 bomapin (PI10) 映射到位于 18q21.3 的 serpin 簇。  |  Bartuski, AJ., et al. 1997. Genomics. 43: 321-8. PMID: 9268635
  7. 人蛋白酶抑制剂 8 对可溶性重组呋喃酶的抑制作用。  |  Dahlen, JR., et al. 1998. J Biol Chem. 273: 1851-4. PMID: 9442015

订购信息

产品名称产品编号规格价格数量收藏夹

SerpinB8 抗体 (PI-8)

sc-101371
200 µg/ml
RMB2377.00

SerpinB8 (PI-8): m-IgG Fc BP-HRP 套装

sc-527066
200 µg Ab; 10 µg BP
RMB2662.00

SerpinB8 (PI-8): m-IgG1 BP-HRP 套装

sc-532439
200 µg Ab; 20 µg BP
RMB2662.00

SerpinB8 抗体 (PI-8) AC

sc-101371 AC
500 µg/ml, 25% agarose
RMB3129.00

SerpinB8 抗体 (PI-8) HRP

sc-101371 HRP
200 µg/ml
RMB2377.00

SerpinB8 抗体 (PI-8) FITC

sc-101371 FITC
200 µg/ml
RMB2482.00

SerpinB8 抗体 (PI-8) PE

sc-101371 PE
200 µg/ml
RMB2580.00

SerpinB8 抗体 (PI-8) Alexa Fluor® 488

sc-101371 AF488
200 µg/ml
RMB2685.00

SerpinB8 抗体 (PI-8) Alexa Fluor® 546

sc-101371 AF546
200 µg/ml
RMB2685.00

SerpinB8 抗体 (PI-8) Alexa Fluor® 594

sc-101371 AF594
200 µg/ml
RMB2685.00

SerpinB8 抗体 (PI-8) Alexa Fluor® 647

sc-101371 AF647
200 µg/ml
RMB2685.00

SerpinB8 抗体 (PI-8) Alexa Fluor® 680

sc-101371 AF680
200 µg/ml
RMB2685.00

SerpinB8 抗体 (PI-8) Alexa Fluor® 790

sc-101371 AF790
200 µg/ml
RMB2685.00

Can SerpinB8 (PI-8): sc-101371 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, SerpinB8 (PI-8): sc-101371 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from WesternblotGood quality Mab.Used it in WB,tried dilution 1:200 and 1:500, bot strong signal for human colon cell lines detection.
Date published: 2018-01-27
Rated 5 out of 5 by from Used for western blot analysis of SerpinB8Used for western blot analysis of SerpinB8 expression in mucosa oropharynx, spleen, placenta, pituitary glad, epidermis, stomach, colon, lung, kidney, liver, heart, brain and pancreas human tissue lysates. Reproduced, with permission, from Strik MC, Bladergroen BA, Wouters D, Kisiel W, Hooijberg JH, Verlaan AR, Hordijk PL, Schneider P, Hack CE, Kummer JA: Distribution of the human intracellular serpin protease inhibitor 8 in human tissues. Journal of Histochemistry & Cytochemistry. 50(11):1443-54, 2002. -SCBT QC
Date published: 2015-03-27
Rated 5 out of 5 by from Produced beautiful immunoperoxidase nuclearProduced beautiful immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human esophagus. -SCBT QC
Date published: 2015-03-03
Rated 5 out of 5 by from Works well for western blotting in humanWorks well for western blotting in human SerpinB8 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-02-06
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SerpinB8 抗体 (PI-8) is rated 5.0 out of 5 by 4.
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