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Septin 10 抗体 (A-2): sc-365775

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说明书
  • Septin 10 抗体 A-2 是小鼠单克隆 IgG2b (kappa轻链),Septin 10抗体,规格为200 µg/ml
  • 免疫原氨基酸序列1-45位于human物种的Septin 10的N-terminus
  • Septin 10 抗体 (A-2) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 Septin 10 isoforms 1 and 2
  • 抗Septin 10抗体(A-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Septin 10 (A-2): sc-365775免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成Septin 10 抗体 (A-2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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    関連項目

    Septin 10抗体(A-2)是一种IgG2b κ小鼠单克隆Septin 10抗体(也称为SEPT10抗体),可通过WB、IP、IF和ELISA检测人类来源的Septin 10蛋白。Septin 10抗体(A-2)既可作为非共轭抗Septin 10抗体形式,也可作为多种共轭形式的抗Septin 10抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。septins是一类GTP酶,其中一些是细胞分裂所必需的,而另一些则与胞吐作用有关。septin家族的成员可以形成异聚体复合物,并在生物体的新生长组织的构成中发挥作用。所有septins的转录调控都很复杂,会产生可选择的剪接变体。Septin 10是一种含有517个残基的多肽,定位于细胞质和细胞核,与Septin 6和Septin 8具有最密切的同源性。Septin 10在生物体中普遍表达,但在胎盘、肺、肾、心脏、骨骼肌、肝脏和各种肿瘤细胞系中表达最丰富。与其他septin家族成员一样,Septin 10具有GTP结合和GTP酶活性。此外,Septin 10可能参与细胞分裂。在脂多糖(LPS)诱导成熟后,树突状细胞表达上调的Septin 10。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Septin 10 抗体 (A-2) 参考文献:

    1. 赛普特蛋白:高度保守的膜缔合 GTP 酶家族,在细胞分裂及其他方面具有功能。  |  Trimble, WS. 1999. J Membr Biol. 169: 75-81. PMID: 10341029
    2. 隔蛋白在哺乳动物细胞分裂机制中的作用  |  Kinoshita, M. and Noda, M. 2001. Cell Struct Funct. 26: 667-70. PMID: 11942624
    3. 人类septin-septin相互作用:CDCrel-1 与 KIAA0202 的相互作用  |  Bläser, S., et al. 2002. FEBS Lett. 519: 169-72. PMID: 12023038
    4. 从树突状细胞中克隆的septin家族新成员--人septin 10的克隆和功能特征。  |  Sui, L., et al. 2003. Biochem Biophys Res Commun. 304: 393-8. PMID: 12711328
    5. 细胞蛋白:细胞分裂交叉点的交通管制。  |  Joo, E., et al. 2005. Traffic. 6: 626-34. PMID: 15998319
    6. 赛普特蛋白可能构成了一个无处不在的细胞骨架细丝家族。  |  Cooper, JA. and Kiehart, DP. 1996. J Cell Biol. 134: 1345-8. PMID: 8830765

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Septin 10 抗体 (A-2)

    sc-365775
    200 µg/ml
    RMB2377.00

    Septin 10 抗体 (A-2) AC

    sc-365775 AC
    500 µg/ml, 25% agarose
    RMB3129.00

    Septin 10 抗体 (A-2) HRP

    sc-365775 HRP
    200 µg/ml
    RMB2377.00

    Septin 10 抗体 (A-2) FITC

    sc-365775 FITC
    200 µg/ml
    RMB2482.00

    Septin 10 抗体 (A-2) PE

    sc-365775 PE
    200 µg/ml
    RMB2580.00

    Septin 10 抗体 (A-2) Alexa Fluor® 488

    sc-365775 AF488
    200 µg/ml
    RMB2685.00

    Septin 10 抗体 (A-2) Alexa Fluor® 546

    sc-365775 AF546
    200 µg/ml
    RMB2685.00

    Septin 10 抗体 (A-2) Alexa Fluor® 594

    sc-365775 AF594
    200 µg/ml
    RMB2685.00

    Septin 10 抗体 (A-2) Alexa Fluor® 647

    sc-365775 AF647
    200 µg/ml
    RMB2685.00

    Septin 10 抗体 (A-2) Alexa Fluor® 680

    sc-365775 AF680
    200 µg/ml
    RMB2685.00

    Septin 10 抗体 (A-2) Alexa Fluor® 790

    sc-365775 AF790
    200 µg/ml
    RMB2685.00

    What is the recommended starting dilution for Western blot for Septin 10 (A-2): sc-365775 monoclonal antibody?

    Asked by: Dr Ninau Qelp
    Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-365775.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
    Answered by: Technical Support
    Date published: 2017-03-01
    • y_2025, m_9, d_7, h_4CST
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    Rated 5 out of 5 by from WBGood Western Blot results of Septin expression in Huh7 cells,1:600 dilution was used.
    Date published: 2018-09-15
    Rated 4 out of 5 by from ImmunostainingImmunostaining of In human cells with septin 10 shows is working good
    Date published: 2017-11-01
    Rated 5 out of 5 by from Septin 10Excelent antibody for western blot. Detected Septin-10 at expected size in 15 ug total proteine lysate, wet transfer one hour at 450 mAmps on PVDF membrane, overnight blocking with 5% nonfat dry milk in PBS, one hour incubation at RT with 1:500 anti Septin-10 in 5% PBS with 0,2%Tween followed by one hour incubation with 1:10.000 anti mouse (sc-358914) HRP conjugated antibody and detection with ECL prime substrate followed by short film exposure (under 1 minute) and automatic processing.
    Date published: 2017-09-25
    Rated 5 out of 5 by from Produced nice Western blot data of SeptinProduced nice Western blot data of Septin 10 expression in Caki-1 whole cell lysate. -SCBT QC
    Date published: 2014-05-26
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    Septin 10 抗体 (A-2) is rated 4.8 out of 5 by 4.
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