Date published: 2025-9-7
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SEMA3A Antibody (A-12) - Western Blotting - Image 378847
SEMA3A 抗体 (A-12): sc-74554. T98G (A), H4 (B) 和 SK-N-MC (C) 全细胞裂解液中 SEMA3A 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
SEMA3A 抗体 (A-12): sc-74554. T98G (A), H4 (B) 和 SK-N-MC (C) 全细胞裂解液中 SEMA3A 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

SEMA3A 抗体 (A-12): sc-74554

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说明书
  • SEMA3A 抗体 A-12 是小鼠单克隆 IgG2b κ，SEMA3A抗体, 在10篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Semaphorin 3A的氨基酸103-402
  • SEMA3A 抗体 (A-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 SEMA3A
  • 抗SEMA3A抗体（A-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SEMA3A (A-12): sc-74554的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 SEMA3A 抗体 (A-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SEMA3A 抗体 (A-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Semaphorin 抗体 包括 SEMA, SEMA4C, SEMA4G, SEMA6A, SEMA6B, SEMA6C, SEMA6D和SEMA7A

SEMA3A抗体（A-12）是一种IgG2b κ小鼠单克隆SEMA3A抗体（也被称为信号素-3A抗体或SEMAD抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的SEMA3A蛋白。SEMA3A抗体（A-12）既有非偶联形式的抗SEMA3A抗体，也有多种偶联形式的抗SEMA3A抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。信号素（SEMAs）由一系列从昆虫到人类都保守存在的细胞表面和分泌蛋白组成。该家族蛋白的成员大约由750个氨基酸组成（包括信号序列），其定义为一个保守的细胞外"信号素"结构域，大约由500个氨基酸组成，包含14-16个半胱氨酸，许多保守序列块，且没有明显的重复。已知分泌型和细胞结合型信号素在化学上可以吸引和排斥神经轴突的生长，引导复杂神经网络的发展。除了它们在轴突导向中的作用外，信号素也与癌症的发展有关。许多信号素与受体神经纤毛蛋白和神经纤毛蛋白-2结合，从而与VEGF竞争这种相互作用，最终导致血管生成的抑制。功能型信号素的丧失与VEGF的竞争可能在肿瘤发展中起重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SEMA3A 抗体 (A-12) 参考文献:

  1. 前裂定位需要半角蛋白 3B 的双重功能活性。  |  Falk, J., et al. 2005. Neuron. 48: 63-75. PMID: 16202709
  2. 上皮性卵巢肿瘤中的半等位蛋白, 血管内皮生长因子及其共同受体神经纤毛蛋白的表达以及半等位蛋白基因座的等位基因缺失：血管内皮生长因子与半等位蛋白比例的增加是卵巢癌的不良预后因素。  |  Osada, R., et al. 2006. Hum Pathol. 37: 1414-25. PMID: 17010410
  3. Semaphorin 3A对骨骼和软骨代谢的影响：基本机制和临床潜力。  |  Li, Z., et al. 2017. Front Cell Neurosci. 11: 40. PMID: 28293171
  4. 孤立性皮肤恶性神经鞘瘤。免疫组织化学和超微结构研究。  |  Zhang, X., et al. 2024. Oral Dis. 30: 1887-1896. PMID: 37771213
  5. Semaphorin 3A在骨骼系统中的作用。  |  Jeon, HM., et al. 2023. JCI Insight. 8: PMID: 37788099
  6. 大鼠S-100α和β亚基mRNA的组织分布。  |  Sang, Y., et al. 2023. Diagnostics (Basel). 13: PMID: 37835781
  7. 小鼠半aphorin D/collapsin是一个多样化基因家族的成员，可创建抑制轴突延伸的结构域。  |  Püschel, AW., et al. 1995. Neuron. 14: 941-8. PMID: 7748561
  8. Semaphorin III 可作为一种选择性趋光剂，对脊髓中的感觉投射进行模式化。  |  Messersmith, EK., et al. 1995. Neuron. 14: 949-59. PMID: 7748562
  9. 轴突导向：排斥生长锥的有力证据  |  Dodd, J. and Schuchardt, A. 1995. Cell. 81: 471-4. PMID: 7758101
  10. Semaphorin II 可作为特定突触分支的选择性抑制剂发挥作用。  |  Matthes, DJ., et al. 1995. Cell. 81: 631-9. PMID: 7758117
  11. semaphorin基因编码一系列跨膜和分泌型生长锥导向分子。  |  Kolodkin, AL., et al. 1993. Cell. 75: 1389-99. PMID: 8269517
  12. 引导分子 semaphorin III 表达于脊髓和外周区域，这些区域被不断生长的感觉轴突所避开。  |  Wright, DE., et al. 1995. J Comp Neurol. 361: 321-33. PMID: 8543665

订购信息

产品名称产品编号规格价格数量收藏夹

SEMA3A 抗体 (A-12)

sc-74554
200 µg/ml
RMB2377.00

SEMA3A (A-12): m-IgG Fc BP-HRP 套装

sc-528691
200 µg Ab; 10 µg BP
RMB2662.00

SEMA3A (A-12): m-IgGκ BP-HRP 套装

sc-521141
200 µg Ab, 40 µg BP
RMB2662.00

SEMA3A (A-12): m-IgG2b BP-HRP 套装

sc-550066
200 µg Ab; 10 µg BP
RMB2662.00

SEMA3A 抗体 (A-12) AC

sc-74554 AC
500 µg/ml, 25% agarose
RMB3129.00

SEMA3A 抗体 (A-12) HRP

sc-74554 HRP
200 µg/ml
RMB2377.00

SEMA3A 抗体 (A-12) FITC

sc-74554 FITC
200 µg/ml
RMB2482.00

SEMA3A 抗体 (A-12) PE

sc-74554 PE
200 µg/ml
RMB2580.00

SEMA3A 抗体 (A-12) Alexa Fluor® 488

sc-74554 AF488
200 µg/ml
RMB2685.00

SEMA3A 抗体 (A-12) Alexa Fluor® 546

sc-74554 AF546
200 µg/ml
RMB2685.00

SEMA3A 抗体 (A-12) Alexa Fluor® 594

sc-74554 AF594
200 µg/ml
RMB2685.00

SEMA3A 抗体 (A-12) Alexa Fluor® 647

sc-74554 AF647
200 µg/ml
RMB2685.00

SEMA3A 抗体 (A-12) Alexa Fluor® 680

sc-74554 AF680
200 µg/ml
RMB2685.00

SEMA3A 抗体 (A-12) Alexa Fluor® 790

sc-74554 AF790
200 µg/ml
RMB2685.00

Instead of old c-17 antibody, this new antibody, as it's written that the molecular weight of sema family is around 80-140kDa. Does it mean this antibody is not specific to sema3a? which protein the 140kDa represent for?

Asked by: luoyuan123
Thank you for your question. The Sema3a protein can also show up at higher molecular weights than the different human and murine isoforms between 80 and 90 kDa. More information can be found in PMID 15485501: “The 125 kDa form detected in hippocampus probably originates in cells bearing the dendritic punctate surface pattern that are observed in subiculum. The 160 kDa and higher mass forms were observed mainly in adult aged hippocampus and may be related to Sema3A bands of similar mass observed [in PMID 9118808] in lectin‐enriched extracts from adult chicken brain” For more information please contact our technical service directly.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Can SEMA (A-12): sc-74554 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, SEMA (A-12): sc-74554 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from WBの結果は大変良好でした膵臓がん細胞株の細胞から抽出したタンパクを18μg泳動し、データーシートにあるように100倍希釈で、1.5時間室温でシェイクし、2次抗体10000倍希釈で1時間反応させた結果、80kDa あたりに、クリアなバンドを確認することが出来た。
Date published: 2022-01-05
Rated 1 out of 5 by from IF failed on mouse brain tissue.Tested IF on frozen sections from embryonic mouse brain, no specific signal was detected.
Date published: 2019-01-02
Rated 5 out of 5 by from Great SEMA antibodyThis SEMA antibody works well in mouse brain tissues. Strong band and clean background
Date published: 2017-11-03
Rated 5 out of 5 by from Strong antibody for IHC and WBSuperb antibody for SEMA research, great for both Western blot and paraffin tissue staining. Great choice!
Date published: 2017-02-08
Rated 4 out of 5 by from nice WB resulti buy a TLR4 antibody in satacruze 2 months ago, it shows a nice WB result and IP result. so i apply a Sema antibody sample for my expertiment, it also shows a good WB result and IP result.
Date published: 2016-12-16
Rated 5 out of 5 by from Produced great immunoperoxidase cytoplasmicProduced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-04-27
Rated 5 out of 5 by from Positive result in WB in T98G whole cell lysatePositive result in WB in T98G whole cell lysate. -SCBT QC
Date published: 2015-04-22
Rated 5 out of 5 by from ClearClear, crisp band detected in WB in H4, SK-N-MC and T3 671 subline no.2 whole cell lysates. -SCBT QC
Date published: 2015-02-06
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SEMA3A 抗体 (A-12) is rated 4.4 out of 5 by 8.
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