Date published: 2025-9-7
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Sec31A Antibody (H-2) - Western Blotting - Image 377712
Sec31A 抗体 (H-2): sc-376587. Sec31A 在 HeLa (A) 和 MCF7 (B) 全细胞裂解液以及人大脑皮层组织提取物 (C) 中表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
Sec31A 抗体 (H-2): sc-376587. Sec31A 在 HeLa (A) 和 MCF7 (B) 全细胞裂解液以及人大脑皮层组织提取物 (C) 中表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

Sec31A 抗体 (H-2): sc-376587

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寫評論提問

说明书
  • Sec31A 抗体 H-2 是小鼠单克隆 IgG2a κ，Sec31A抗体, 在9篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列429-571位于human物种的Sec31A的内部区域内
  • Sec31A 抗体 (H-2) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 Sec31A
  • 抗Sec31A抗体（H-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Sec31A (H-2): sc-376587的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是Sec31A Antibody (H-2) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Sec31A Antibody (H-2) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Sec抗体，包括Sec10, Sec24D, Sec31A, Sec5, Sec6, Sec61 α, Sec61 β, Sec63和Sec8

Sec31A抗体（H-2）是一种IgG2a κ小鼠单克隆Sec31A抗体（也被称为Sec31A抗体），它通过WB、IP、IF和ELISA检测方法识别人类来源的Sec31A蛋白。Sec31A抗体（H-2）既可以作为非偶联形式的抗Sec31A抗体使用，也可以作为多种偶联形式的抗Sec31A抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。WD重复序列是在多种蛋白质中发现的模体，其特征是由40-60个氨基酸组成的保守核心，通常形成三级螺旋桨结构。虽然含有WD重复序列的蛋白质参与广泛的细胞功能，但它们通常与染色质组装、细胞周期控制、信号转导、RNA加工、凋亡和囊泡运输等调控机制有关。Sec31A，也被称为ABP125、ABP130或SEC31L1，是一个由1220个氨基酸组成的蛋白质，包含七个WD重复序列，并定位于细胞质和细胞质囊泡，以及内质网（ER）膜上。Sec31A普遍高水平表达，作为COP II（外被蛋白II）复合物的组成部分，并与其他蛋白质协同工作，促进ER运输囊泡的形成，并有助于货物分子的选择。涉及Sec31A基因的染色体畸变与炎症性肌纤维母细胞瘤（IMTs）相关，提示Sec31A在致癌作用中发挥作用。由于选择性剪接事件的存在，Sec31A存在多种异构体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Sec31A 抗体 (H-2) 参考文献:

  1. 酵母 sec31p 的哺乳动物同源物。一种普遍表达的形式定位于内质网（ER）出口位点，对ER-高尔基体转运至关重要。  |  Tang, BL., et al. 2000. J Biol Chem. 275: 13597-604. PMID: 10788476
  2. 活细胞中分泌蛋白的运输和细胞器的动力学。  |  Lippincott-Schwartz, J., et al. 2000. Annu Rev Cell Dev Biol. 16: 557-89. PMID: 11031247
  3. 制作囊泡的三种方法  |  Kirchhausen, T. 2000. Nat Rev Mol Cell Biol. 1: 187-98. PMID: 11252894
  4. 炎性肌纤维母细胞瘤中的 SEC31L1 和 ALK 基因融合。  |  Panagopoulos, I., et al. 2006. Int J Cancer. 118: 1181-6. PMID: 16161041
  5. Sec13/31 COPII 涂层笼的结构。  |  Stagg, SM., et al. 2006. Nature. 439: 234-8. PMID: 16407955
  6. Ca2+结合蛋白ALG-2被Sec31A招募到内质网出口位点，并稳定Sec31A的定域。  |  Yamasaki, A., et al. 2006. Mol Biol Cell. 17: 4876-87. PMID: 16957052
  7. Sec23-Sec24 与 Sec13-Sec31 的高效耦合驱动了 COPII 依赖性胶原蛋白分泌，对正常颅面发育至关重要。  |  Townley, AK., et al. 2008. J Cell Sci. 121: 3025-34. PMID: 18713835

订购信息

产品名称产品编号规格价格数量收藏夹

Sec31A 抗体 (H-2)

sc-376587
200 µg/ml
RMB2377.00

Sec31A (H-2): m-IgG Fc BP-HRP 套装

sc-529908
200 µg Ab; 10 µg BP
RMB2662.00

Sec31A (H-2): m-IgGκ BP-HRP 套装

sc-523020
200 µg Ab, 40 µg BP
RMB2662.00

Sec31A (H-2): m-IgG2a BP-HRP 套装

sc-547399
200 µg Ab; 10 µg BP
RMB2662.00

Sec31A 抗体 (H-2) AC

sc-376587 AC
500 µg/ml, 25% agarose
RMB3129.00

Sec31A 抗体 (H-2) HRP

sc-376587 HRP
200 µg/ml
RMB2377.00

Sec31A 抗体 (H-2) FITC

sc-376587 FITC
200 µg/ml
RMB2482.00

Sec31A 抗体 (H-2) PE

sc-376587 PE
200 µg/ml
RMB2580.00

Sec31A 抗体 (H-2) Alexa Fluor® 488

sc-376587 AF488
200 µg/ml
RMB2685.00

Sec31A 抗体 (H-2) Alexa Fluor® 546

sc-376587 AF546
200 µg/ml
RMB2685.00

Sec31A 抗体 (H-2) Alexa Fluor® 594

sc-376587 AF594
200 µg/ml
RMB2685.00

Sec31A 抗体 (H-2) Alexa Fluor® 647

sc-376587 AF647
200 µg/ml
RMB2685.00

Sec31A 抗体 (H-2) Alexa Fluor® 680

sc-376587 AF680
200 µg/ml
RMB2685.00

Sec31A 抗体 (H-2) Alexa Fluor® 790

sc-376587 AF790
200 µg/ml
RMB2685.00

What is the recommend I.F dilution for this antibody?

Asked by: Richik
Thank you for your question. We recommend a starting dilution of 1:50 with this mouse monoclonal, Sec31A Antibody (H-2): sc-376587. The typical range of dilutions we recommend testing for optimization is 1:50-1:500.
Answered by: Technical Support
Date published: 2017-04-17

What is the recommended fixation method for immunofluorescence staining with Sec31A (H-2): sc-376587 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good antibody for WBI used this AB for WesternBlots with MCF7 whole cell lysate : good signal detected by ECL, but some unspecific weak bands.
Date published: 2020-07-31
Rated 5 out of 5 by from Works for western blot /MCF7 cell extractsI used this antibody in a 1:1000 dilution in TBST-5%milk, on MCF7 cell extracts. It produces a clear band at the expected size (ECL reagent).
Date published: 2020-02-14
Rated 5 out of 5 by from Perfect antibody to COP-II vesicle marker (Sec31)I used this clone successfully for immunocytostaining, western blotting of human and mouse cell and tissues. For staining of cartilages this antibody totally co-localized with Sec23a which is also COP-II vesicle marker. So this antibody is very useful for multiple application.
Date published: 2019-08-28
Rated 5 out of 5 by from good for immunofluorescenceIt was used in immuofluorescence 1:100 on primary endothelial cells fixed by PFA
Date published: 2018-05-04
Rated 5 out of 5 by from sec31抗体很有效做Western Blot很好，条带比较清晰，杂带很少，位是置也对，在130kd的位置。在INS1细胞和293T细胞中 做的
Date published: 2018-03-25
Rated 4 out of 5 by from GoodTested this antibody for IF at 1:50-75 dilution on PFA fixed HeLa cells. The results were fair enough.
Date published: 2017-05-05
Rated 5 out of 5 by from Specific COP-II vesicle staining with Sec23aIt worked in both 4% PFA and cold MeOH-fixed cells and it perfectly colocalized with sec23a which is same COP-II component. It looked like dot like structures.
Date published: 2017-02-02
Rated 5 out of 5 by from Quality IF stainingBright and clear staining seen in IF with methanol fixed cells.
Date published: 2017-01-19
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Sec31A 抗体 (H-2) is rated 4.8 out of 5 by 12.
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