Date published: 2026-10-24

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SDHB Antibody (G-10) - Western Blotting - Image 355515
SDHB 抗体 (G-10): sc-271548. U-698-M (A), Jurkat (B) 和 A-431 (C) 全细胞裂解液及小鼠肝组织提取物 (D) 中 SDHB 表达的 Western 印迹分析.
SDHB 抗体 (G-10): sc-271548. U-698-M (A), Jurkat (B) 和 A-431 (C) 全细胞裂解液及小鼠肝组织提取物 (D) 中 SDHB 表达的 Western 印迹分析.

SDHB 抗体 (G-10): sc-271548

4.2(21)
寫評論提問
说明书
  • SDHB 抗体 G-10 是小鼠单克隆 IgG1 κ，SDHB抗体, 在21篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1-280的抗性，代表全长琥珀酸脱氢酶铁硫蛋白，human起源
  • SDHB 抗体 (G-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 precursor and mature SDHB
  • 抗SDHB抗体（G-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是SDHB Antibody (G-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与SDHB Antibody (G-10) 打包提供（请参阅下面的订购信息）。
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関連項目

额外SDH抗体，包括SDHB

SDHB抗体（G-10）是一种IgG1κ小鼠单克隆SDHB抗体（也称为琥珀酸脱氢酶复合物铁硫亚基B抗体、复合物II的铁硫亚基抗体、琥珀酸脱氢酶[泛醌]铁硫亚基线粒体抗体、琥珀酸脱氢酶复合物亚基B铁硫（Ip）抗体、SDH1抗体、SDH抗体、SDHIP抗体、CWS2抗体、PGL4抗体或SDH2抗体），可以通过WB、IP、IF和ELISA等方法检测来自小鼠、大鼠和人类的SDHB蛋白。SDHB抗体（G-10）有两种形式：非偶联型抗SDHB抗体以及多种偶联型抗SDHB抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。在有氧呼吸反应中，琥珀酸脱氢酶（SDH）催化琥珀酸和泛醌氧化为延胡索酸和泛醇。SDH蛋白复合物由四个亚基组成：一个黄素蛋白亚基（SDHA）、一个铁硫蛋白（SDHB）和两个膜结合亚基（SDHC和SDHD），它们锚定在线粒体内膜上。这些亚基的突变会导致线粒体功能障碍，与几种不同的疾病相对应。膜结合组分的突变可能导致遗传性副神经节瘤，而SDHA突变与青少年脑病以及Leigh综合征（一种严重的神经系统疾病）有关。SDHB中的失活突变与遗传性（但不一定是散发性）嗜铬细胞瘤病例相关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SDHB 抗体 (G-10) 参考文献:

  1. 琥珀酸脱氢酶亚基 SDHB 的基因突变导致家族性嗜铬细胞瘤和家族性副神经节瘤的易感性。  |  Astuti, D., et al. 2001. Am J Hum Genet. 69: 49-54. PMID: 11404820
  2. 家族性嗜铬细胞瘤和副神经节瘤中出现新的琥珀酸脱氢酶亚基 B (SDHB) 突变，但散发性嗜铬细胞瘤中没有体细胞 SDHB 突变。  |  Benn, DE., et al. 2003. Oncogene. 22: 1358-64. PMID: 12618761
  3. 肾细胞癌中 FIH-1, FH 和 SDHB 候选肿瘤抑制基因的分子遗传分析。  |  Morris, MR., et al. 2004. J Clin Pathol. 57: 706-11. PMID: 15220362
  4. 与 SDHB 基因种系突变有关的恶性嗜铬细胞瘤。  |  Allibhai, Z., et al. 2004. J Urol. 172: 1409-10. PMID: 15371856
  5. 琥珀酸脱氢酶亚基 B（SDHB）的缺失是晚期回肠分化良好的神经内分泌肿瘤的预后因素。  |  Milione, M., et al. 2017. Endocrine. 57: 512-517. PMID: 27905048
  6. 与琥珀酸脱氢酶亚基基因 SDHB、SDHC 和 SDHD 的种系变异有关的肿瘤风险和基因型-表型相关性。  |  Andrews, KA., et al. 2018. J Med Genet. 55: 384-394. PMID: 29386252
  7. SDHB 的缺失会导致铁平衡失调、氧化应激和对抗坏血酸的敏感性。  |  Goncalves, J., et al. 2021. Cancer Res. 81: 3480-3494. PMID: 34127497
  8. 量化罕见表型的致病性证据：琥珀酸脱氢酶基因 SDHB 和 SDHD 的案例。  |  Garrett, A., et al. 2022. Genet Med. 24: 41-50. PMID: 34906457
  9. 关于 SDHB 基因突变及相关疾病发病机制的研究进展。  |  Liu, C., et al. 2023. Biomed Pharmacother. 167: 115500. PMID: 37734265
  10. 种系 SDHB 致病变体患者的嗜铬细胞瘤和副神经节瘤的管理：国际专家共识声明。  |  Taïeb, D., et al. 2024. Nat Rev Endocrinol. 20: 168-184. PMID: 38097671
  11. 核琥珀酸脱氢酶基因突变导致线粒体呼吸链缺乏。  |  Bourgeron, T., et al. 1995. Nat Genet. 11: 144-9. PMID: 7550341
  12. 人类复合体 II（琥珀酸-泛醌氧化还原酶）：肝线粒体黄蛋白（Fp）亚基的 cDNA 克隆。  |  Hirawake, H., et al. 1994. J Biochem. 116: 221-7. PMID: 7798181

订购信息

产品名称产品编号规格价格数量收藏夹

SDHB 抗体 (G-10)

sc-271548
200 µg/ml
CNY2422.00

SDHB (G-10): m-IgG Fc BP-HRP 套装

sc-529209
200 µg Ab; 10 µg BP
CNY2715.00

SDHB (G-10): m-IgGκ BP-HRP 套装

sc-521977
200 µg Ab, 40 µg BP
CNY2715.00

SDHB (G-10): m-IgG1 BP-HRP 套装

sc-543387
200 µg Ab; 20 µg BP
CNY2715.00

SDHB 抗体 (G-10) AC

sc-271548 AC
500 µg/ml, 25% agarose
CNY3189.00

SDHB 抗体 (G-10) HRP

sc-271548 HRP
200 µg/ml
CNY2422.00

SDHB 抗体 (G-10) FITC

sc-271548 FITC
200 µg/ml
CNY2527.00

SDHB 抗体 (G-10) PE

sc-271548 PE
200 µg/ml
CNY2625.00

SDHB 抗体 (G-10) Alexa Fluor® 488

sc-271548 AF488
200 µg/ml
CNY2738.00

SDHB 抗体 (G-10) Alexa Fluor® 546

sc-271548 AF546
200 µg/ml
CNY2738.00

SDHB 抗体 (G-10) Alexa Fluor® 594

sc-271548 AF594
200 µg/ml
CNY2738.00

SDHB 抗体 (G-10) Alexa Fluor® 647

sc-271548 AF647
200 µg/ml
CNY2738.00

SDHB 抗体 (G-10) Alexa Fluor® 680

sc-271548 AF680
200 µg/ml
CNY2738.00

SDHB 抗体 (G-10) Alexa Fluor® 790

sc-271548 AF790
200 µg/ml
CNY2738.00

What is the recommended fixation method for immunofluorescence staining with SDHB (G-10): sc-271548 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by Chia87 from It worksI tried a sample and it works fine 1:500 with 20 micrograms of proteins.
Date published: 2023-09-20
Rated 1 out of 5 by moirr01 from doesn't work in westernOther mitochondrial antibodies from Santa Cruz Biotechnology work well for westerns of mouse cerebral cortex. This one does not generate any signal at all.
Date published: 2021-10-27
Rated 2 out of 5 by Linlin1 from Don't work well!Without clean Western blot band in LO2 cells extract，diluent at 1:200
Date published: 2020-05-17
Rated 1 out of 5 by karra from no bands appearedWe used both dillutions 1/250 and 1/500 but bands didn't appear in neither.
Date published: 2019-04-01
Rated 5 out of 5 by Hongx from Great antibody for SDHB.I got this free trial sample, and worked in HepG2 and BV2 cells. Works well.
Date published: 2019-03-01
Rated 5 out of 5 by CMTatMCCT from works well on WBThis antibody gives clean background on Western blots.
Date published: 2018-06-22
Rated 5 out of 5 by lixueguang from Good AntibodySDHB antibody has very nice western blot results in mouse liver tissue extract, strong signal with low background and the baseband is perfect.
Date published: 2017-10-22
Rated 5 out of 5 by mayr from SDHB antibody (G-10)I tested this antibody in 2% PFA-fixed and permeabilized (0.3% TritonX-100) cells, and it worked very well at 1:200 dilution.
Date published: 2017-09-26
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SDHB 抗体 (G-10) is rated 4.2 out of 5 by 21.
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