Date published: 2025-9-11
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SBP Tag 抗体 (SB19-C4): sc-101595

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说明书
  • SBP Tag 抗体 SB19-C4 是小鼠单克隆 IgG1 κ，SBP Tag抗体, 在58篇文献中引用，规格为200 µg/ml
  • 针对链霉亲和素结合肽
  • SBP Tag 抗体 (SB19-C4) 推荐用于 WB 和 IF，检测 SBP tag
  • SBP Tag抗体（SB19-C4）可与琼脂糖结合用于IP，与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SBP Tag (SB19-C4): sc-101595的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成SBP Tag 抗体 (SB19-C4)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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研究信息

関連項目

SBP Tag 抗体 (SB19-C4) 是一种 IgG1 κ 小鼠单克隆 SBP Tag 抗体（也称为链霉亲和素结合肽抗体、SBP-Tag 抗体、38 氨基酸 SBP 标记抗体、SBP 抗体或 MDEKTTGWRGGHVVEGLAGEELQLRARLEHHPQGQREP 抗体），可通过 WB 和 IF 检测 SBP Tag 蛋白。SBP Tag 抗体 (SB19-C4) 既有非结合型的抗 SBP Tag 抗体，也有多种结合型的抗 SBP Tag 抗体，包括琼脂糖、PE、FITC 和多种 Alexa Fluor® 结合物。链霉亲和素是从链霉菌 avidinii 中纯化出来的一种四聚体蛋白，与生物素的结合非常紧密，Kd 为 10-14 mol/l，是已知的最强的生物和非共价相互作用之一。每个链霉亲和素单体可结合一分子生物素。强力的链霉亲和素-生物素键可用于将各种化学物质 "粘 "在表面上，并将放射性同位素和单克隆抗体等分子连接在一起。链霉亲和素广泛应用于科学实验室，通常用于纯化免疫化学试剂，也是诊断和实验室试剂盒中最重要的成分之一。SBP Tag（Streptavidin binding protein Tag）是一种能与 Streptavidin 结合的 38 氨基酸蛋白亲和序列，可用于检测和纯化各种重组蛋白。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SBP Tag 抗体 (SB19-C4) 参考文献:

  1. 使用纳摩尔亲和力链霉亲和素结合肽 SBP-Tag 一步法纯化重组蛋白。  |  Keefe, AD., et al. 2001. Protein Expr Purif. 23: 440-6. PMID: 11722181
  2. 蛋白质之间的配体交换生物素和生物素衍生物在阿维菌素和链霉亲和素之间的交换。  |  Pazy, Y., et al. 2002. J Biol Chem. 277: 30892-900. PMID: 12055191
  3. 链霉亲和素结合肽标记委内瑞拉马脑炎病毒单链可变片段抗体的基因工程。  |  Hu, WG., et al. 2002. Hybrid Hybridomics. 21: 415-20. PMID: 12573105
  4. Nano-tag 是一种链霉亲和素结合肽，用于纯化和检测重组蛋白。  |  Lamla, T. and Erdmann, VA. 2004. Protein Expr Purif. 33: 39-47. PMID: 14680960
  5. 重组表达链霉亲和素的有利溶解伙伴。  |  Sørensen, HP., et al. 2003. Protein Expr Purif. 32: 252-9. PMID: 14965771
  6. 链霉亲和素-生物素系统中的氢键相互作用。  |  Hyre, DE., et al. 2006. Protein Sci. 15: 459-67. PMID: 16452627
  7. 具有单个飞摩尔生物素结合位点的单价链霉亲和素。  |  Howarth, M., et al. 2006. Nat Methods. 3: 267-73. PMID: 16554831
  8. 与链霉亲和素结合的 (+)-epi 生物素的高分辨率结构。  |  Le Trong, I., et al. 2006. Acta Crystallogr D Biol Crystallogr. 62: 576-81. PMID: 16699183
  9. 通过链霉亲和素-琼脂糖牵引分析蛋白质与 DNA 的结合。  |  Wu, KK. 2006. Methods Mol Biol. 338: 281-90. PMID: 16888365
  10. 链霉亲和素结合肽（SBP）标记的SMC2允许一步法亲和荧光、印迹或纯化压缩蛋白复合物。  |  Kim, JH., et al. 2010. BMC Biochem. 11: 50. PMID: 21194474
  11. 结构导向的链霉亲和素工程化设计，可重复用于纯化链霉亲和素结合肽标记的蛋白或生物素化蛋白。  |  Wu, SC. and Wong, SL. 2013. PLoS One. 8: e69530. PMID: 23874971
  12. 使用链霉亲和素结合肽 (SBP) 标签对 ERK 信号复合物进行单步亲和纯化。  |  Yang, L. and Veraksa, A. 2017. Methods Mol Biol. 1487: 113-126. PMID: 27924562

订购信息

产品名称产品编号规格价格数量收藏夹

SBP Tag 抗体 (SB19-C4)

sc-101595
200 µg/ml
RMB2377.00

SBP Tag 抗体 (SB19-C4) AC

sc-101595 AC
500 µg/ml, 25% agarose
RMB3129.00

SBP Tag 抗体 (SB19-C4) FITC

sc-101595 FITC
200 µg/ml
RMB2482.00

SBP Tag 抗体 (SB19-C4) PE

sc-101595 PE
200 µg/ml
RMB2580.00

SBP Tag 抗体 (SB19-C4) Alexa Fluor® 488

sc-101595 AF488
200 µg/ml
RMB2685.00

SBP Tag 抗体 (SB19-C4) Alexa Fluor® 546

sc-101595 AF546
200 µg/ml
RMB2685.00

SBP Tag 抗体 (SB19-C4) Alexa Fluor® 594

sc-101595 AF594
200 µg/ml
RMB2685.00

SBP Tag 抗体 (SB19-C4) Alexa Fluor® 647

sc-101595 AF647
200 µg/ml
RMB2685.00

SBP Tag 抗体 (SB19-C4) Alexa Fluor® 680

sc-101595 AF680
200 µg/ml
RMB2685.00

SBP Tag 抗体 (SB19-C4) Alexa Fluor® 790

sc-101595 AF790
200 µg/ml
RMB2685.00

I am looking for an antibody that recognizes the SBP tag and can be used for immunoprecipitation (ChIP). Will this antibody work? If not, could you point me to one that will?

Asked by: MTStan
Thank you for your question. This antibody has not yet been tested for IP or ChIP applications. We are aware of a couple of product citations using this antibody for IP (PMID's: 2402161 and 25412508), but nothing yet for ChIP. The agarose conjugated antibody is recommended and warrantied for immunoprecipitation (IP), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2020-02-06

I have (sc-101595) SBP Tag-HRP ab. What is the dilution you recommend for ELISA? Thanks,

Asked by: yangm6
Thank you for your question. We do not have established dilutions for ELISA for our antibodies as they require titration. You can find our ELISA protocol on the website here: https://www.scbt.com/scbt/resources/protocols/elisa-assays
Answered by: Technical Service
Date published: 2017-07-14

I have run out of the goat anti-mouse secondary antibody I usually use for WB detection. What do you recommend I use?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-30
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Rated 4 out of 5 by from Clean backgroundThe antibody didn't detect the bands I expected to see, but I believe that my sample is more responsible than the antibody. The background, however, was much cleaner than other strep-tag antibodies.
Date published: 2017-09-06
Rated 5 out of 5 by from clear stripsI used it at 1:500 for western blots, and the result is very good.
Date published: 2017-09-05
Rated 5 out of 5 by from Good resultsI got strong band at dilution 1:500, my experimental result was very well.
Date published: 2017-05-13
Rated 5 out of 5 by from positive resultsThis antibody was used in WB is excellent, strong signal
Date published: 2017-04-24
Rated 5 out of 5 by from Good for wbWe used this in WB and we got a strong signal and clean background.
Date published: 2017-02-07
Rated 5 out of 5 by from Published antibodyPublished in several publications with drosophila and human samples by IP, WB and IF
Date published: 2017-01-13
Rated 5 out of 5 by from Good wbGood for western blot application;includes full set of diterct conjugates
Date published: 2017-01-01
Rated 4 out of 5 by from Used it in WB and worked well at 1:250 dilutionUsed it in WB and worked well at 1:250 dilution.
Date published: 2015-06-16
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SBP Tag 抗体 (SB19-C4) is rated 4.4 out of 5 by 10.
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