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用法 :
Gram stain procedure
• Prepare and heat-fix bacterial smears.
• Flood the slides crystal violet for one minute.
• Pour off excess dye and wash gently in tap water and drain the slide against a paper towel.
• Expose the smears to Gram's iodine (sc-358803) for one minute by washing with iodine, then adding more iodine and leaving it on the smear until the minute is over.
• Wash with tap water and drain carefully. (Do not blot.)
• Wash with 95% alcohol for 30 seconds.
• Wash with tap water at the end of the 30 seconds to stop the decolorization. Drain.
• Counterstain with 25% Safranin T(sc-203758) for 30 seconds.
• Wash, drain, blot, and examine under oil.
外观 :
Powder
物理状态 :
Solid
溶解度 :
Soluble in water (50 mg/ml at 20° C), and alcohol.