Date published: 2025-9-7
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SA-2 Antibody (J-12) - Western Blotting - Image 33499
SA-2 抗体 (J-12): sc-81852. 对 HeLa 核提取物中 SA-2 的表达进行 Western 印迹分析.
SA-2 抗体 (J-12): sc-81852. 对 HeLa 核提取物中 SA-2 的表达进行 Western 印迹分析.

SA-2 抗体 (J-12): sc-81852

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说明书
  • SA-2抗体(J-12)是小鼠单克隆IgG1 κ, 在41篇文献中引用，规格为100 µg/ml
  • 针对重组蛋白的抗体，其表位位于human的SA-2的C-terminus
  • 推荐用于 mouse, rat 和 human 来源的SA-2 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取SA-2 (J-12): sc-81852的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 SA-2 抗体 (J-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SA-2 抗体 (J-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
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研究信息

関連項目

此外 SA 抗体包括 SA-1 和 SA-2

SA-2抗体（J-12）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人类样本中的SA-2。SA-2（J-12）抗体以非结合形式提供，可满足各种实验需求。SA-2（也称为STAG2）是凝聚复合物中一个关键的1231个氨基酸的组成部分，在确保细胞分裂过程中姐妹染色单体正确分离方面发挥着至关重要的作用。SA-2定位于细胞核中，与染色质结合并维持基因组稳定性。在受到Polo样激酶（Plk）磷酸化后，SA-2与染色质分离，从而促进染色体在后期正确分离。SA-2已被证明能够增强肿瘤坏死因子α（TNFα）的活性，表明其可能具有转录调节器的潜在作用，这可能对癌症生物学和治疗策略产生影响。SA-2与Rad21和凝聚复合物其他成分的相互作用凸显了SA-2在细胞周期调节和染色体完整性中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SA-2 抗体 (J-12) 参考文献:

  1. 脊椎动物凝聚蛋白复合物的特征及其在前期的调控。  |  Sumara, I., et al. 2000. J Cell Biol. 151: 749-62. PMID: 11076961
  2. STAG2 和 Rad21 哺乳动物有丝分裂粘合蛋白与减数分裂有关。  |  Prieto, I., et al. 2002. EMBO Rep. 3: 543-50. PMID: 12034751
  3. 证明粘合素 STAG/SA/Scc3 具有转录共激活剂功能。  |  Lara-Pezzi, E., et al. 2004. J Biol Chem. 279: 6553-9. PMID: 14660624
  4. 有丝分裂早期，凝聚素与染色体臂的分离以及染色体臂凝聚力的丧失取决于 SA2 的磷酸化。  |  Hauf, S., et al. 2005. PLoS Biol. 3: e69. PMID: 15737063
  5. 在脊椎动物细胞的有丝分裂过程中，舒戈欣能防止凝聚素与中心粒分离。  |  McGuinness, BE., et al. 2005. PLoS Biol. 3: e86. PMID: 15737064
  6. 粘合蛋白存在于与禽类灯泡染色体缔合作用的中心粒蛋白体中。  |  Krasikova, A., et al. 2005. Chromosome Res. 13: 675-85. PMID: 16235117
  7. Ewing肉瘤的基因组图谱定义了一种具有STAG2和TP53突变共同关联的侵袭性亚型。  |  Tirode, F., et al. 2014. Cancer Discov. 4: 1342-53. PMID: 25223734
  8. STAG2在膀胱癌中的作用。  |  Aquila, L., et al. 2018. Pharmacol Res. 131: 143-149. PMID: 29501732
  9. Cohesin成员Stag1和Stag2在染色质可及性和HSC自我更新和分化的拓扑控制中发挥不同的作用。  |  Viny, AD., et al. 2019. Cell Stem Cell. 25: 682-696.e8. PMID: 31495782
  10. STAG2的缺失重构了致癌和发育程序，促进了尤文肉瘤的转移。  |  Adane, B., et al. 2021. Cancer Cell. 39: 827-844.e10. PMID: 34129824
  11. STAG2 通过增强子环路重编程调节黑色素瘤中的干扰素信号。  |  Chu, Z., et al. 2022. Nat Commun. 13: 1859. PMID: 35388001
  12. STAG2调节同源重组修复和对ATM抑制的敏感性。  |  Zhou, J., et al. 2023. Adv Sci (Weinh). 10: e2302494. PMID: 37985839

订购信息

产品名称产品编号规格价格数量收藏夹

SA-2 抗体 (J-12)

sc-81852
100 µg/ml
$333.00

SA-2 (J-12): m-IgG Fc BP-HRP 套装

sc-539668
100 µg Ab; 10 µg BP
$354.00

SA-2 (J-12): m-IgG1 BP-HRP 套装

sc-541647
100 µg Ab; 20 µg BP
$354.00

Hi Sir/ Mrs, I am interested in STAG2 antibody, but I do need to know the sequence of the antigen you used to make this antibody because I need it to do matching with STAG2 of dog. I need to know if this human STAG2 is suitable for dog...Thanks a lot.

Asked by: IJLafta
Thank you for your question. The specific antigen used to make this antibody, SA-2 (J-12): sc-81852, is considered proprietary. Please contact our Technical Service Department and we are happy to perform the sequence analysis for you to predict whether or not the antibody may cross-react with STAG2 of dog origin. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2018-01-16

I am wondering if you have STAG2 (SA-2) monoclonal antibody that is suitable to be used in IHC of dog tissues.   Thanks D. Inam Lafta

Asked by: I J L
Thank you for your question. This monoclonal antibody, SA-2 Antibody (J-12): sc-81852, has not been tested for reactivity with dog, and we are not aware of any publications citing use of the antibody in this species. Unfortunately, none of our SA-2 antibodies have been tested in, or are currently recommended for detection of the protein in dog tissues.
Answered by: Technical Support
Date published: 2017-09-28

Do you recommend using this mouse monoclonal SA-2 antibody for staining mouse tissue?

Asked by: cjMara
Thank you for your question. Yes, SA-2 Antibody (J-12): sc-81852 can be used for IF and IHC(P) on mouse tissue. We recommend using our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP): sc-516102 for specific detection of the primary antibody with minimal background.
Answered by: Technical Support 13
Date published: 2017-01-09
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Rated 5 out of 5 by from good quality antibodyGives good and specific signal for both unmodified and mono-ubiquitinated SA-2 on WB (use no less than 1:800).
Date published: 2017-06-03
Rated 5 out of 5 by from Great productStrong, specific western data; also nice product for IHC and IF staining applications
Date published: 2017-01-26
Rated 5 out of 5 by from Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-01-09
Rated 4 out of 5 by from Nice band seen in WB using HeLa nuclear extractNice band seen in WB using HeLa nuclear extract. -SCBT QC
Date published: 2015-07-14
Rated 4 out of 5 by from Publishable data in WB using human H4 cellsPublishable data in WB using human H4 cells -SCBT Publication Review
Date published: 2015-06-25
Rated 4 out of 5 by from Various publishable data in IHC(P) usingVarious publishable data in IHC(P) using human H4 cells and bladder tumors. -SCBT Publication Review
Date published: 2015-06-10
Rated 4 out of 5 by from Robust immunoperoxidase nuclear stainingRobust immunoperoxidase nuclear staining in formalin-fixed, paraffin-embedded human lymph node tissue. -SCBT QC
Date published: 2015-03-18
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SA-2 抗体 (J-12) is rated 4.4 out of 5 by 7.
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