Date published: 2025-9-8

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RUNX3 抗体 (R3-5G4): sc-101553

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说明书
  • RUNX3 抗体 R3-5G4 是小鼠单克隆 IgG1 κ,RUNX3抗体, 在13篇文献中引用,规格为200 µg/ml
  • 免疫human物种的recombinant RUNX3的氨基酸191-300
  • RUNX3 抗体 (R3-5G4) 推荐用于WB, IP, IF 和 IHC(P),检测human 来源的 RUNX3
  • 抗RUNX3抗体(R3-5G4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RUNX3 (R3-5G4): sc-101553免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRPm-IgGκ BP-HRP 是 RUNX3 抗体 (R3-5G4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与RUNX3 抗体 (R3-5G4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

RUNX3抗体(R3-5G4)是一种IgG1 κ小鼠单克隆RUNX3抗体(也称作CBFA3抗体、AML2抗体或PEBP2A3抗体),它能够通过WB、IP、IF和IHC(P)检测人源RUNX3蛋白。RUNX3抗体(R3-5G4)既有非偶联形式的抗RUNX3抗体,也有多种偶联形式的抗RUNX3抗体,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®偶联物。哺乳动物Runt相关转录因子(RUNX)家族包括三个成员:RUNX1(也称作AML-1、PEBP2αB、CBFA2)、RUNX2(也称作AML-3、PEBP2αA、CBFA1、Osf2)和RUNX3(也称作AML-2、PEBPαC、CBFA3),属于急性髓系白血病(AML)家族。RUNX家族成员是DNA结合蛋白,调节参与细胞分化和细胞周期进展的基因的表达。RUNX3在造血起源的细胞中表达,包括髓系和B细胞系以及脾脏。RUNX3在控制胃上皮细胞生长和分化中发挥作用,是胃癌抑癌基因的有力候选者。具体来说,超甲基化会使编码RUNX3的基因失活。检测RUNX3 CpG岛内多个区域的超甲基化可能有助于胃癌的诊断和风险评估。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RUNX3 抗体 (R3-5G4) 参考文献:

  1. 通过视黄酸受体α依赖性信号通路调节造血细胞中的 AML2/CBFA3  |  Le, XF., et al. 1999. J Biol Chem. 274: 21651-8. PMID: 10419474
  2. RUNX 基因家族的致癌潜力:'概述'。  |  Ito, Y. 2004. Oncogene. 23: 4198-208. PMID: 15156173
  3. 肺癌中 RUNX3 基因的表观遗传失活。  |  Sato, K., et al. 2006. Oncol Rep. 15: 129-35. PMID: 16328045
  4. 胃癌中 RUNX3 CpG 岛内甲基化的扩散。  |  Homma, N., et al. 2006. Cancer Sci. 97: 51-6. PMID: 16367921
  5. Runx 蛋白在软骨形成过程中的作用  |  Yoshida, CA. and Komori, T. 2005. Crit Rev Eukaryot Gene Expr. 15: 243-54. PMID: 16390320
  6. Runx3 控制着哺乳动物胃上皮细胞的生长和分化。  |  Fukamachi, H. 2006. Dev Growth Differ. 48: 1-13. PMID: 16466388
  7. Runx3 控制 CD8+ T 细胞在非淋巴组织中的驻留。  |  Milner, JJ., et al. 2017. Nature. 552: 253-257. PMID: 29211713
  8. Runx3 的表观遗传重编程可增强 CD8+ T 细胞的功能,提高免疫治疗的临床反应。  |  Liu, Z., et al. 2023. Mol Cancer. 22: 84. PMID: 37189103
  9. 哺乳动物 Runt 结构域第三个编码基因 PEBP2 alpha C 的克隆, 绘图和表达。  |  Bae, SC., et al. 1995. Gene. 159: 245-8. PMID: 7622058
  10. AML-2是急性白血病中t(8;21)和t(12;21)融合蛋白转录调控的潜在靶标。  |  Meyers, S., et al. 1996. Oncogene. 13: 303-12. PMID: 8710369
  11. 与人类白血病有关的新转录因子家族。  |  Speck, NA. and Terryl, S. 1995. Crit Rev Eukaryot Gene Expr. 5: 337-64. PMID: 8834230
  12. 髓性白血病中 3;21 易位的 AML1/CBFA2 基因重排:体外和体内研究。  |  Zent, C., et al. 1997. Leukemia. 11 Suppl 3: 273-8. PMID: 9209363

订购信息

产品名称产品编号规格价格数量收藏夹

RUNX3 抗体 (R3-5G4)

sc-101553
200 µg/ml
RMB2377.00

RUNX3 (R3-5G4): m-IgGκ BP-HRP 套装

sc-521200
200 µg Ab, 40 µg BP
RMB2662.00

RUNX3 (R3-5G4): m-IgG1 BP-HRP 套装

sc-533482
200 µg Ab; 20 µg BP
RMB2662.00

RUNX3 抗体 (R3-5G4) AC

sc-101553 AC
500 µg/ml, 25% agarose
RMB3129.00

RUNX3 抗体 (R3-5G4) HRP

sc-101553 HRP
200 µg/ml
RMB2377.00

RUNX3 抗体 (R3-5G4) FITC

sc-101553 FITC
200 µg/ml
RMB2482.00

RUNX3 抗体 (R3-5G4) PE

sc-101553 PE
200 µg/ml
RMB2580.00

RUNX3 抗体 (R3-5G4) Alexa Fluor® 488

sc-101553 AF488
200 µg/ml
RMB2685.00

RUNX3 抗体 (R3-5G4) Alexa Fluor® 546

sc-101553 AF546
200 µg/ml
RMB2685.00

RUNX3 抗体 (R3-5G4) Alexa Fluor® 594

sc-101553 AF594
200 µg/ml
RMB2685.00

RUNX3 抗体 (R3-5G4) Alexa Fluor® 647

sc-101553 AF647
200 µg/ml
RMB2685.00

RUNX3 抗体 (R3-5G4) Alexa Fluor® 680

sc-101553 AF680
200 µg/ml
RMB2685.00

RUNX3 抗体 (R3-5G4) Alexa Fluor® 790

sc-101553 AF790
200 µg/ml
RMB2685.00

Hallo, ich würde gerne ein 10 µg GRATIS Sample vom RUNX3 (R3-5G4): sc-101553 erhalten. Ist das möglich? MfG, Anja Nitzsche

Asked by: Anja Nitzsche
Sehr geehrte Frau Nitzsche, vielen Dank für Ihre Anfrage. Wir freuen uns wenn Sie unsere Samples testen möchten. Dazu brauchen wir Ihre Adresse sowie Telefonnummer. Bitte schreiben Sie uns ihre Kontaktdaten an [email protected] oder rufen uns an unter der Telefonnummer: 06221 4503 0.
Answered by: Technical Support Europe
Date published: 2017-08-29

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with RUNX3 (R3-5G4): sc-101553 monoclonal antibody?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2017-01-18
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Rated 5 out of 5 by from 试用RUNX3抗体抗体效果特别好!特异性强!淋巴细胞强阳性,周围上皮阴性。抗体效果特别好!特异性强!淋巴细胞强阳性,周围上皮阴性。
Date published: 2018-04-03
Rated 5 out of 5 by from Great western and IHCGreat western blot data; product also has superb IHC results
Date published: 2017-01-31
Rated 5 out of 5 by from Works Great!This antibody works great for western blot analysis of RUNX3 expression in MEG-01 nuclear extract and U266 whole cell lysate.
Date published: 2016-12-22
Rated 5 out of 5 by from Superb immunoperoxidase nuclear stainingSuperb immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human skin tissue. -SCBT QC
Date published: 2015-04-26
Rated 5 out of 5 by from Robust signal in WB in MEGRobust signal in WB in MEG-01 nuclear extract and U266 whole cell lysate. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Good publishable data in WB in human HEKGood publishable data in WB in human HEK-293 cells. -SCBT Publication Review
Date published: 2015-02-02
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RUNX3 抗体 (R3-5G4) is rated 5.0 out of 5 by 6.
  • y_2025, m_8, d_30, h_9
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