Date published: 2025-9-8
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RPA 32 kDa subunit Antibody (9H8) - Western Blotting - Image 19886
RPA 32 kDa 亚基抗体 (9H8): sc-56770. H69AR (A), HeLa (B), Saos-2 (C), T-47D (D) 和 MCF7 (E) 全细胞裂解液中 RPA 32 kDa 亚基表达的 Western 印迹分析.
RPA 32 kDa 亚基抗体 (9H8): sc-56770. H69AR (A), HeLa (B), Saos-2 (C), T-47D (D) 和 MCF7 (E) 全细胞裂解液中 RPA 32 kDa 亚基表达的 Western 印迹分析.

RPA 32 kDa subunit 抗体 (9H8): sc-56770

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说明书
  • RPA 32 kDa subunit 抗体 9H8 是小鼠单克隆 IgG1 κ，RPA 32 kDa subunit抗体, 在43篇文献中引用，规格为200 µg/ml
  • 针对全长RPA 32 kDa subunit，human起源
  • RPA 32 kDa subunit 抗体 (9H8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 RPA 32 kDa subunit
  • 抗RPA 32 kDa subunit抗体（9H8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RPA 32 kDa subunit (9H8): sc-56770的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 RPA 32 kDa subunit 抗体 (9H8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与RPA 32 kDa subunit 抗体 (9H8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Replication Protein A 抗体包括 RPA 14 kDa subunit, RPA 32 kDa subunit 和 RPA 70 kDa subunit

RPA 32 kDa 亚基抗体 (9H8) 是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF)、石蜡包埋切片免疫组织化学 (IHCP) 和酶联免疫吸附试验 (ELISA) 检测小鼠、大鼠和人源的 RPA 32 kDa 亚基蛋白。RPA 32 kDa 亚基（9H8）抗体有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 共轭物。RPA 32 kDa 亚基是复制蛋白 A（RPA）复合物的重要组成部分，对真核细胞中的 DNA 复制、重组和修复至关重要。RPA 32 kDa 亚基与细胞核中的单链 DNA 结合，稳定 DNA 并防止二级结构妨碍复制和修复过程。RPA 32 kDa 亚基的核定位允许在关键的细胞过程中有效地访问 DNA，确保基因组的稳定性和完整性。RPA 32 kDa 亚基与参与 DNA 修复的各种蛋白质（包括 UNG2、XPA 和 Rad52）相互作用，协调细胞对 DNA 损伤的反应。RPA 32 kDa 亚基与 Stat3 的 SH2 结构域特异性结合，表明它参与了调节细胞生长和存活的信号通路，这使得 RPA 32 kDa 亚基在 DNA 维护和细胞信号传导方面都具有重要意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RPA 32 kDa subunit 抗体 (9H8) 参考文献:

  1. 与 RFA1 基因相互作用的特征：RPA 在 DNA 复制和端粒维持中的作用。  |  Smith, J., et al. 2000. Biochimie. 82: 71-8. PMID: 10717390
  2. RPA 通过蛋白质与蛋白质之间的相互作用，稳定 XPA 破坏的 DNA 复合物。  |  Wang, M., et al. 2000. Biochemistry. 39: 6433-9. PMID: 10828957
  3. 复制蛋白 a 32 kDa 亚基（RPA p32）与 STAT3 的 SH2 结构域结合，并调节其转录活性。  |  Kim, J., et al. 2000. Cell Biol Int. 24: 467-73. PMID: 10875894
  4. 人类复制蛋白 A（hRPA）与伪原 DNA 底物结合的 5' --> 3' 分子极性。  |  Iftode, C. and Borowiec, JA. 2000. Biochemistry. 39: 11970-81. PMID: 11009611
  5. 复制因子 RPA 识别 DNA 修复蛋白 UNG2, XPA 和 RAD52 的结构基础。  |  Mer, G., et al. 2000. Cell. 103: 449-56. PMID: 11081631
  6. 编码人类复制蛋白 A 的 70 kDa 单链 DNA 结合亚基的 cDNA 的特征及其在 DNA 复制中的作用。  |  Erdile, LF., et al. 1991. J Biol Chem. 266: 12090-8. PMID: 2050703
  7. 人类复制蛋白 A 32 kDa 亚基的主要结构。  |  Erdile, LF., et al. 1990. J Biol Chem. 265: 3177-82. PMID: 2406247
  8. 与 DNA 结合的复制蛋白 A 单链 DNA 结合域的结构。  |  Bochkarev, A., et al. 1997. Nature. 385: 176-81. PMID: 8990123

订购信息

产品名称产品编号规格价格数量收藏夹

RPA 32 kDa subunit 抗体 (9H8)

sc-56770
200 µg/ml
RMB2377.00

RPA 32 kDa subunit (9H8): m-IgG Fc BP-HRP 套装

sc-528620
200 µg Ab; 10 µg BP
RMB2662.00

RPA 32 kDa subunit (9H8): m-IgGκ BP-HRP 套装

sc-521044
200 µg Ab, 40 µg BP
RMB2662.00

RPA 32 kDa subunit (9H8): m-IgG1 BP-HRP 套装

sc-543032
200 µg Ab; 20 µg BP
RMB2662.00

RPA 32 kDa subunit 抗体 (9H8) AC

sc-56770 AC
500 µg/ml, 25% agarose
RMB3129.00

RPA 32 kDa subunit 抗体 (9H8) HRP

sc-56770 HRP
200 µg/ml
RMB2377.00

RPA 32 kDa subunit 抗体 (9H8) FITC

sc-56770 FITC
200 µg/ml
RMB2482.00

RPA 32 kDa subunit 抗体 (9H8) PE

sc-56770 PE
200 µg/ml
RMB2580.00

RPA 32 kDa subunit 抗体 (9H8) Alexa Fluor® 488

sc-56770 AF488
200 µg/ml
RMB2685.00

RPA 32 kDa subunit 抗体 (9H8) Alexa Fluor® 546

sc-56770 AF546
200 µg/ml
RMB2685.00

RPA 32 kDa subunit 抗体 (9H8) Alexa Fluor® 594

sc-56770 AF594
200 µg/ml
RMB2685.00

RPA 32 kDa subunit 抗体 (9H8) Alexa Fluor® 647

sc-56770 AF647
200 µg/ml
RMB2685.00

RPA 32 kDa subunit 抗体 (9H8) Alexa Fluor® 680

sc-56770 AF680
200 µg/ml
RMB2685.00

RPA 32 kDa subunit 抗体 (9H8) Alexa Fluor® 790

sc-56770 AF790
200 µg/ml
RMB2685.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with RPA 32 kDa subunit Antibody (9H8): sc-56770?

Asked by: jenniferc
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-10
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Rated 5 out of 5 by from Excellent antibody for IFI used this antibody at a 1:200 dilution on pancreatic cancer cell lines. It works well and gives repeatable results in IF. I haven't used it for Western blot yet.
Date published: 2023-08-03
Rated 5 out of 5 by from Works great for western blotThis is one of our commonly used antibody that works really well, especially as a control following probing with other phospho-specific RPA antibodies on western blot. Working dilution 1:6000 - 1:8000 depending on the amount of protein loaded.
Date published: 2021-11-04
Rated 5 out of 5 by from RPA32 (9H8) antibodyWorked very well for western blot. The signal was strong and specific.
Date published: 2019-04-09
Rated 5 out of 5 by from Great as loading controlThis antibody works very well. I have used it as loading control diluted 1:1000 in 5% skimmed milk.
Date published: 2018-10-29
Rated 4 out of 5 by from works well for IFgood detection of RPA32 foci in HEp-2 cells after etoposide treatment however for good detection, the antibody has to be used at 1:75 dilution in BSA for immunofluorescence staining
Date published: 2018-08-01
Rated 5 out of 5 by from Fantastic for WBWorks great for WB at 1:1000 dilution in HeLa and 293T cells.
Date published: 2017-09-06
Rated 5 out of 5 by from Good feature!A strong band corresponding to topBP1 expression was detected with this antibody from the whole cell extract of Hela cells.
Date published: 2017-08-25
Rated 5 out of 5 by from Good antibody for western blotThe antibody detects a single band of the right size from HuH7 cell lysates at a 1:8000X dilution. It may work with an even higher dilution ratio.
Date published: 2017-07-26
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RPA 32 kDa subunit 抗体 (9H8) is rated 4.8 out of 5 by 16.
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