Date published: 2025-9-7

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Rock-1 抗体 (G-6): sc-17794

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说明书
  • Rock-1抗体(G-6)是小鼠单克隆IgG1 κ, 在92篇文献中引用,规格为200 µg/ml
  • 免疫human来源Rock-1内部区域的755-840氨基酸
  • 抗-Rock-1 抗体 (G-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Rock-1
  • 抗Rock-1抗体(G-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rock-1 (G-6): sc-17794免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 Rock-1 抗体 (G-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Rock-1 抗体 (G-6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Rock-1抗体(G-6)是一种鼠单克隆IgG1 kappa轻链抗体,专门设计用于检测小鼠、大鼠和人类等多种物种中的Rho相关、含有螺旋线圈的蛋白激酶1(Rock-1)。Rock-1单克隆抗体(G-6)针对人类Rock-1蛋白755-840氨基酸内的表位,确保在应用中精确检测,如蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)。Rock-1是一种重要的丝氨酸/苏氨酸激酶,参与调节肌动蛋白细胞骨架动态,控制应力纤维、粘着斑和收缩环等结构,这些结构对于细胞分裂和细胞运动等过程至关重要。作为小GTP酶Rho的关键效应因子,Rock-1在维持细胞形状、激活影响细胞增殖和凋亡的信号转导通路以及促进细胞结构完整性方面发挥着关键作用。Rock-1 (G-6) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物,可满足各种实验需求。Anti-Rock-1抗体(G-6)有助于研究重要的信号传导通路,以及与细胞骨架异常和细胞运动失调相关的疾病。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rock-1 抗体 (G-6) 参考文献:

  1. 冠状动脉疾病患者的RhoA/ROCK-1信号通路和氧化应激。  |  Dokumacioglu, E., et al. 2022. Braz J Cardiovasc Surg. 37: 212-218. PMID: 34236817
  2. 胆固醇瘤患者中的RhoA、ROCK-1和ROCK-2基因表达和多态性。  |  Görür, K., et al. 2021. J Int Adv Otol. 17: 530-535. PMID: 35177391
  3. ROCK 1和2以不同方式影响源自人角膜基质成纤维细胞的3D球体的空间结构。  |  Ida, Y., et al. 2022. Sci Rep. 12: 7419. PMID: 35523828
  4. RhoA/ROCK抑制可减轻内皮素-1诱导的大鼠肾小球病变。  |  Saleh, MA., et al. 2023. Life Sci. 323: 121687. PMID: 37030613
  5. 一种新型丝氨酸/苏氨酸激酶与 Ras 相关的 RhoA GTPase 结合,从而将激酶转运到外周膜。  |  Leung, T., et al. 1995. J Biol Chem. 270: 29051-4. PMID: 7493923
  6. 大鼠睾丸脂肪酸活化蛋白激酶(PKN)的纯化和表征。  |  Kitagawa, M., et al. 1995. Biochem J. 310 (Pt 2): 657-64. PMID: 7654208
  7. 蛋白激酶 N(PKN)和 PKN 相关蛋白 rhophilin 是小 GTPase Rho 的靶标。  |  Watanabe, G., et al. 1996. Science. 271: 645-8. PMID: 8571126
  8. 确定丝氨酸-苏氨酸激酶蛋白激酶 N 是 Rho 的假定靶标。  |  Amano, M., et al. 1996. Science. 271: 648-50. PMID: 8571127
  9. PKN氨基末端,N端独特基团对其酶活性的作用。  |  Kitagawa, M., et al. 1996. Biochem Biophys Res Commun. 220: 963-8. PMID: 8607876
  10. 小 GTP 结合蛋白 Rho 与一个与肌营养不良症激酶同源的 160 kDa Ser/Thr 蛋白激酶结合并激活该激酶。  |  Ishizaki, T., et al. 1996. EMBO J. 15: 1885-93. PMID: 8617235
  11. PKN 与神经丝蛋白的头棒结构域结合并使其磷酸化。  |  Mukai, H., et al. 1996. J Biol Chem. 271: 9816-22. PMID: 8621664
  12. RhoA 与 PKN 氨基末端,N 端之间相互作用的特征。  |  Shibata, H., et al. 1996. FEBS Lett. 385: 221-4. PMID: 8647255

订购信息

产品名称产品编号规格价格数量收藏夹

Rock-1 抗体 (G-6)

sc-17794
200 µg/ml
RMB2377.00

Rock-1 (G-6): m-IgG Fc BP-HRP 套装

sc-525486
200 µg Ab; 10 µg BP
RMB2662.00

Rock-1 抗体 (G-6) AC

sc-17794 AC
500 µg/ml, 25% agarose
RMB3129.00

Rock-1 抗体 (G-6) HRP

sc-17794 HRP
200 µg/ml
RMB2377.00

Rock-1 抗体 (G-6) FITC

sc-17794 FITC
200 µg/ml
RMB2482.00

Rock-1 抗体 (G-6) PE

sc-17794 PE
200 µg/ml
RMB2580.00

Rock-1 抗体 (G-6) Alexa Fluor® 488

sc-17794 AF488
200 µg/ml
RMB2685.00

Rock-1 抗体 (G-6) Alexa Fluor® 546

sc-17794 AF546
200 µg/ml
RMB2685.00

Rock-1 抗体 (G-6) Alexa Fluor® 594

sc-17794 AF594
200 µg/ml
RMB2685.00

Rock-1 抗体 (G-6) Alexa Fluor® 647

sc-17794 AF647
200 µg/ml
RMB2685.00

Rock-1 抗体 (G-6) Alexa Fluor® 680

sc-17794 AF680
200 µg/ml
RMB2685.00

Rock-1 抗体 (G-6) Alexa Fluor® 790

sc-17794 AF790
200 µg/ml
RMB2685.00

Can you recommend a positive control to use with Rock-1 (G-6): sc-17794 for Western blot?

Asked by: jenni
We recommend Caki-1 (sc-2224), Hep G2 (sc-2227), NIH/3T3 (sc-2210) or KNRK (sc-2214) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of Rock-1 in these cell lines.
Answered by: Technical Support 13
Date published: 2023-04-10

How can Rock-1 (G-6): sc-17794 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. Rock-1 (G-6): sc-17794 monoclonal antibody is currently available conjugated to phycoerythrin (sc-17794 PE), fluorescein (sc-17794 FITC), Alexa Fluor® 488 (sc-17794 AF488) or Alexa Fluor® 647 (sc-17794 AF647)
Answered by: Technical Support 1
Date published: 2023-04-26
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Rated 3 out of 5 by from WB ROCK1Used for detection by immunoblot of human ROCK1: low sensitivity, some background
Date published: 2021-12-02
Rated 5 out of 5 by from Great antibody!I received a sample and it works very well in immunofluorescence
Date published: 2021-10-07
Rated 5 out of 5 by from superb antibodyI got this as a sample,. It works well, so I am buying it
Date published: 2020-08-26
Rated 5 out of 5 by from Great.We use it for the expression using Western blot analysis in HeLa and the products were good.
Date published: 2018-10-12
Rated 5 out of 5 by from worked well in western blotI got the free sample and works very well at 1:200 for BMDM lysate
Date published: 2018-03-23
Rated 4 out of 5 by from Very good in WB!!!I bought it 2 months ago. It works really good at 15ug protein lysate for HCC154 human cell line
Date published: 2018-02-01
Rated 5 out of 5 by from 效果很好该抗体是单克隆抗体,特异性提高了,用于检测人体组织标本效果也很好,检出率比较高,背景不明显。效果不错。
Date published: 2017-09-28
Rated 4 out of 5 by from 检出效果满意申请了该抗体的单克隆试用装,效果不错,但有个问题是对应的分子量和建议的分子量有点出入。条带的显示还好,背景不高。
Date published: 2017-09-25
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Rock-1 抗体 (G-6) is rated 4.7 out of 5 by 31.
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