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RNase L Antibody (2E9.2G5) - Western Blotting - Image 8670 — RNase L 抗体 (2E9. 2G5): sc-23955. DU 145 (A) 和 PC-3 (B) 全细胞裂解液中 RNase L 表达的 Western 印迹分析.

RNase L 抗体 (2E9. 2G5): sc-23955. DU 145 (A) 和 PC-3 (B) 全细胞裂解液中 RNase L 表达的 Western 印迹分析.

RNase L 抗体 (2E9.2G5): sc-23955

Name: RNase L抗体 (2E9.2G5)
Brand: SCBIO
SKU: sc-23955
Price: 2377.0 CNY
Availability: InStock

5.0(2)

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说明书

RNase L抗体(2E9.2G5)是小鼠单克隆IgG₁ κ, 在2篇文献中引用，规格为200 µg/ml
针对纯化的重组human RNase L
推荐用于 human 来源的RNase L WB, IP, IF 和 IHC(P)检测
m-IgG Fc BP-HRP, m-IgG₁ BP-HRP 和 m-IgGκ BP-HRP 是 RNase L 抗体 (2E9.2G5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与RNase L 抗体 (2E9.2G5) 组合成套装提供。 (请参阅下面的订购信息)。

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RNase L 抗体 (2E9.2G5) 参考文献:

RNase-L失调对免疫和增殖控制的病理影响 | Ezelle, HJ. and Hassel, BA. 2012. Front Biosci (Schol Ed). 4: 767-86. PMID: 22202089
RNase-L 对细胞 mRNA 的控制：在生物功能中的作用和底物靶向机制。 | Brennan-Laun, SE., et al. 2014. J Interferon Cytokine Res. 34: 275-88. PMID: 24697205
RNase-L 在抗微生物免疫和细胞骨架相关先天反应中的作用 | Ezelle, HJ., et al. 2016. Int J Mol Sci. 17: PMID: 26760998
作为抗癌剂的 RNase L 结合型 2- 氨基噻吩的合成与评估。 | Hwang, J., et al. 2022. Bioorg Med Chem. 58: 116653. PMID: 35152173
细胞质中的 RNase L 激活会诱导细胞核中 mRNA 的异常处理。 | Burke, JM., et al. 2022. PLoS Pathog. 18: e1010930. PMID: 36318584
轮状病毒 NSP1 通过诱导 RNase L 降解颠覆了抗病毒寡腺苷酸合成酶-RNase L 途径。 | Dai, J., et al. 2022. mBio. 13: e0299522. PMID: 36413023
病毒感染过程中 RNase L 对核糖核蛋白缩聚物的调控 | Burke, JM. 2023. Wiley Interdiscip Rev RNA. 14: e1770. PMID: 36479619
与 SARS-CoV-2 相关的儿童多系统炎症综合征中 OAS-RNase L 的先天性错误。 | Lee, D., et al. 2023. Science. 379: eabo3627. PMID: 36538032
先天免疫内切核酸酶 RNase L 启动 ZAK&alpha 依赖性核糖毒性应激反应 | Xi, J., et al. 2024. Cell Rep. 43: 113998. PMID: 38551960
RNase L诱导体封存亚基因组黄病毒RNA，促进病毒RNA衰变。 | Watkins, JM. and Burke, JM. 2024. Cell Rep. 43: 114694. PMID: 39196777

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
RNase L 抗体 (2E9.2G5)	sc-23955	200 µg/ml	RMB2377.00
RNase L (2E9.2G5): m-IgG Fc BP-HRP 套装	sc-536694	200 µg Ab; 10 µg BP	RMB2662.00
RNase L (2E9.2G5): m-IgGκ BP-HRP 套装	sc-533854	200 µg Ab; 40 µg BP	RMB2662.00
RNase L (2E9.2G5): m-IgG₁ BP-HRP 套装	sc-544770	200 µg Ab; 20 µg BP	RMB2662.00

Can RNase L (2E9.2G5): sc-23955 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc

Thank you for your question. Yes, RNase L (2E9.2G5): sc-23955 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support

Date published: 2017-02-25

Dear Santa Cruz,  I use this antibody to detect endogenous RNase L. Activated RNase L is dimerized and it can show two bands but in case of this antibody is it possible to see difference between activated and non activated RNase L? Thank you!

Asked by: Idee

Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody, RNase L (2E9.2G5): sc-23955, has not been determined. Thus, it would have to be determined experimentally if the antibody can distinguish between activated and non-activated RNase L. This experiment has not been performed by Santa Cruz Biotechnology.

Answered by: Technical Support

Date published: 2020-10-13

Rated 5 out of 5 by HC from Positive results in western blot analysisPositive results in western blot analysis of RNase L expression in DU 145 and PC-3 whole cell lysates. -SCBT QC

Date published: 2015-04-09

Rated 5 out of 5 by JM from Excellent immunoperoxidase cytoplasmic stainingExcellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human liver tissue. -SCBT QC

Date published: 2015-01-27

RNase L 抗体 (2E9.2G5) is rated 5.0 out of 5 by 2.

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RNase L 抗体 (2E9.2G5): sc-23955

RNase L 抗体 (2E9.2G5) 参考文献:

優選的 RNase L 抗體是 RNase L 抗體 (E-9): sc-74405.

RNase L 抗体 (2E9.2G5)

RNase L (2E9.2G5): m-IgG Fc BP-HRP 套装

RNase L (2E9.2G5): m-IgGκ BP-HRP 套装

RNase L (2E9.2G5): m-IgG1 BP-HRP 套装

Can RNase L (2E9.2G5): sc-23955 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Dear Santa Cruz,&nbsp; I use this antibody to detect endogenous RNase L. Activated RNase L is dimerized and it can show two bands but in case of this antibody is it possible to see difference between activated and non activated RNase L? Thank you!

RNase L (2E9.2G5): m-IgG₁ BP-HRP 套装

Dear Santa Cruz, I use this antibody to detect endogenous RNase L. Activated RNase L is dimerized and it can show two bands but in case of this antibody is it possible to see difference between activated and non activated RNase L? Thank you!