Date published: 2025-9-8

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RING1B 抗体 (N-32): sc-101109

3.5(6)
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说明书
  • RING1B 抗体 N-32 是小鼠单克隆 IgG2a κ,RING1B抗体, 在17篇文献中引用,规格为100 µg/ml
  • 免疫human物种的重组RING1B
  • 推荐用于 mouse, rat 和 human 来源的RING1B WB, IP 和 ELISA检测
  • 关于如何获取RING1B (N-32): sc-101109免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是RING1B Antibody (N-32) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与RING1B Antibody (N-32) 打包提供(请参阅下面的订购信息)。

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関連項目

RING1B抗体(N-32)是一种IgG2a κ小鼠单克隆RING1B抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的RING1B蛋白。RING1B抗体(N-32)为非偶联形式的抗RING1B抗体。Polycomb group(PcG)蛋白形成多蛋白复合物,调节发育和细胞增殖基因的表达模式。RING1B(E3泛素蛋白连接酶RING2),也称为RING2、RING finger蛋白BAP-1、DinG蛋白或HIP2相互作用蛋白3,是一种参与蛋白质降解和泛素化途径的PcG蛋白。作为介导组蛋白H2A单泛素化的E3泛素蛋白连接酶,RING1B是染色质相关II类PcG抑制复合物1(PRC1/hPRC-H)的重要组成部分,该复合物在整个发育过程中抑制许多基因的转录。RING1B含有一个RING型锌指结构域,该结构域在E3泛素连接酶活性期间与PRC1复合物内的亨廷顿相互作用蛋白2(HIP2)相互作用。RING1B定位于细胞核内,可能参与雌性哺乳动物X染色体的随机和印记失活。RING1B在小鼠中的表达不足会导致轴性骨骼异常和一些HOX基因的表达减少,而完全缺乏RING1B的小鼠则会出现原肠胚形成停滞。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RING1B 抗体 (N-32) 参考文献:

  1. 与人类泛素结合酶 Hip-2 相互作用的 RING 手指蛋白的 E3 连接酶活性。  |  Lee, SJ., et al. 2001. FEBS Lett. 503: 61-4. PMID: 11513855
  2. RING 多聚核蛋白与发育转录因子谷粒头样家族复合物中的特定靶基因结合。  |  Tuckfield, A., et al. 2002. Mol Cell Biol. 22: 1936-46. PMID: 11865070
  3. 多聚核糖体组基因 Ring1B 参与小鼠前后轴的规范化。  |  Suzuki, M., et al. 2002. Development. 129: 4171-83. PMID: 12183370
  4. Rnf2(Ring1b)缺乏会导致胃形成停滞和细胞周期抑制。  |  Voncken, JW., et al. 2003. Proc Natl Acad Sci U S A. 100: 2468-73. PMID: 12589020
  5. 组蛋白 H2A 泛素化在多聚酶沉默中的作用  |  Wang, H., et al. 2004. Nature. 431: 873-8. PMID: 15386022
  6. 多聚胞群蛋白Ring1A/B将组蛋白H2A的泛素化与遗传性基因沉默和X失活联系起来。  |  de Napoles, M., et al. 2004. Dev Cell. 7: 663-76. PMID: 15525528
  7. 多聚胞复合体抑制小鼠胚胎干细胞中的发育调节因子。  |  Boyer, LA., et al. 2006. Nature. 441: 349-53. PMID: 16625203
  8. 多聚酶蛋白Ring1B产生自身非典型混合泛素链,这是其体外组蛋白H2A连接酶活性所必需的。  |  Ben-Saadon, R., et al. 2006. Mol Cell. 24: 701-711. PMID: 17157253

订购信息

产品名称产品编号规格价格数量收藏夹

RING1B 抗体 (N-32)

sc-101109
100 µg/ml
RMB2504.00

RING1B (N-32): m-IgG Fc BP-HRP 套装

sc-537299
100 µg Ab; 10 µg BP
RMB2662.00

RING1B (N-32): m-IgGκ BP-HRP 套装

sc-534531
100 µg Ab; 40 µg BP
RMB2662.00

RING1B (N-32): m-IgG2a BP-HRP 套装

sc-547969
100 µg Ab; 10 µg BP
RMB2662.00

For Western Blot, is it recommended to use denatured or non-denatured conditions with RING1B (N-32): sc-101109 antibody?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 3 out of 5 by from Work but with specificity issueCertainly can detect endogenous RING1B but with many non-specific bands.
Date published: 2018-04-11
Rated 3 out of 5 by from RING1BWorks but is a little messy, shows up with extra bands
Date published: 2018-03-29
Rated 4 out of 5 by from Good for IP and western blotSuccessfully IPs endogenous RING1B from HEK293T cells. Detected decent and specific band at expected size.
Date published: 2018-01-14
Rated 4 out of 5 by from Good WB antibodyWorks for WB (1:500-1:1000). It is not suitable for IF (lots of background)
Date published: 2017-10-09
Rated 3 out of 5 by from work for Western BlotTried the sample with MCF-7 cell lysate. Has a major band between 37-50kD.
Date published: 2017-04-03
Rated 4 out of 5 by from WB publishable data with human breast cancerWB publishable data with human breast cancer cell lines - SCBT Publication Review
Date published: 2013-03-08
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RING1B 抗体 (N-32) is rated 3.5 out of 5 by 6.
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