Date published: 2025-9-7
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RICK Antibody (A-10) - Western Blotting - Image 373917
RICK 抗体 (A-10): sc-166765. K-562 (A), HeLa (B), Ramos (C), THP-1 (D) 和 Caki-1 (E) 全细胞裂解液中 RICK 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
RICK 抗体 (A-10): sc-166765. K-562 (A), HeLa (B), Ramos (C), THP-1 (D) 和 Caki-1 (E) 全细胞裂解液中 RICK 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

RICK 抗体 (A-10): sc-166765

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说明书
  • RICK 抗体 A-10 是小鼠单克隆 IgG1 κ，RICK抗体, 在7篇文献中引用，规格为200 µg/ml
  • 免疫human物种的RICK的氨基酸241-540.
  • RICK 抗体 (A-10) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 RICK
  • 抗RICK抗体（A-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RICK (A-10): sc-166765的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是RICK Antibody (A-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与RICK Antibody (A-10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

RICK抗体(A-10)是一种IgG1 κ型小鼠单克隆RICK抗体[也称为受体相互作用丝氨酸/苏氨酸激酶2 (RIPK2)抗体、RIP2抗体、含Caspase募集域(CARD3)激酶抗体、受体相互作用蛋白(RIP)家族丝氨酸/苏氨酸蛋白激酶抗体、含羧基端Caspase募集域(CARD)的固有免疫和适应性免疫相关信号蛋白激酶抗体]，或上游NF-κB (NF-κB, NF-κB)活化蛋白抗体)，通过WB、IP、IF和ELISA检测人源RICK蛋白。RICK抗体(A-10)既可以是非偶联的抗RICK抗体形式，也可以是多种偶联的抗RICK抗体形式，包括琼脂糖，HRP, PE, FITC和多个Alexa Fluor®偶联物。肿瘤坏死因子受体(tumor necrosis factor receptor, TNFR)家族成员在诱导NFkB活化和细胞死亡中发挥关键作用。这些受体募集并组装包含大量含死亡结构域蛋白(如RIP)的信号复合体。RICK也被命名为RIP2和CARDIAK，是一种rip样蛋白激酶，参与调节TNFR和cd95介导的细胞凋亡。RICK包含一个n端丝氨酸-苏氨酸激酶催化结构域和一个c端半胱氨酸蛋白酶招募结构域。NFkB的c端结构域足以发挥其凋亡功能，而整个蛋白则是NFkB激活所必需的。RICK与tnfr相关因子(TRA≠F)家族中的多种蛋白特异性结合，这些TRAF相互作用参与了将RICK招募到受体信号复合体中。过表达RICK导致caspase-8活化，增强Fas配体、FADD、CLARP和caspase-8诱导的细胞凋亡。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RICK 抗体 (A-10) 参考文献:

  1. SNO-MLP（肌肉LIM蛋白的S-亚硝基化）通过TLR3（Toll样受体3）介导的RIP3（受体相互作用蛋白激酶3）和NLRP3（NOD样受体皮林结构域含有3）炎症小体激活促进心肌肥大。  |  Tang, X., et al. 2020. Circulation. 141: 984-1000. PMID: 31902237
  2. 坏死介导受体相互作用蛋白激酶 3 在肾损伤中的新作用  |  Uni, R. and Choi, ME. 2022. Nephron. 146: 259-263. PMID: 34284405
  3. Caspase-8阻断胚胎发育过程中受体相互作用蛋白激酶-1激酶依赖性坏死过程  |  Zhang, H., et al. 2022. Immunohorizons. 6: 465-475. PMID: 35858757
  4. 研究受体相互作用蛋白激酶 3 在静脉血栓中的作用。  |  DeRoo, E., et al. 2022. JVS Vasc Sci. 3: 365-378. PMID: 36568281
  5. 受体相互作用蛋白激酶 3 在心血管疾病中的病理生理作用。  |  Zhang, J., et al. 2023. Biomed Pharmacother. 165: 114696. PMID: 37329707
  6. 受体相互作用蛋白激酶2（RIPK2）是导致中风后神经炎症和行为障碍的重要因素，而小胶质细胞是独特的肇事者。  |  Larochelle, J., et al. 2023. J Neuroinflammation. 20: 221. PMID: 37777791
  7. 受体互作蛋白激酶-3 在杜氏肌营养不良症临床前动物模型中介导肌病和心肌病。  |  Bencze, M., et al. 2023. J Cachexia Sarcopenia Muscle. 14: 2520-2531. PMID: 37909859
  8. 作为人类肌萎缩性脊髓侧索硬化症生物标志物的受体互作蛋白激酶 1 (RIPK1) 和 IL-8 的外周水平升高。  |  Wei, J., et al. 2023. Signal Transduct Target Ther. 8: 451. PMID: 38086800
  9. 膀胱癌外泌体circRNA_0013936通过上调脂肪酸转运蛋白2和下调PMN-MDSCs中的受体相互作用蛋白激酶3促进抑制性免疫。  |  Shi, X., et al. 2024. Mol Cancer. 23: 52. PMID: 38461272
  10. 药理抑制受体相互作用蛋白激酶 2 (RIPK2) 可在实验性缺血性中风后产生神经保护作用。  |  Larochelle, J., et al. 2024. Exp Neurol. 377: 114812. PMID: 38729551

订购信息

产品名称产品编号规格价格数量收藏夹

RICK 抗体 (A-10)

sc-166765
200 µg/ml
RMB2377.00

RICK (A-10): m-IgG Fc BP-HRP 套装

sc-529043
200 µg Ab; 10 µg BP
RMB2662.00

RICK (A-10): m-IgGκ BP-HRP 套装

sc-521737
200 µg Ab, 40 µg BP
RMB2662.00

RICK (A-10): m-IgG1 BP-HRP 套装

sc-543286
200 µg Ab; 20 µg BP
RMB2662.00

RICK 抗体 (A-10) AC

sc-166765 AC
500 µg/ml, 25% agarose
RMB3129.00

RICK 抗体 (A-10) HRP

sc-166765 HRP
200 µg/ml
RMB2377.00

RICK 抗体 (A-10) FITC

sc-166765 FITC
200 µg/ml
RMB2482.00

RICK 抗体 (A-10) PE

sc-166765 PE
200 µg/ml
RMB2580.00

RICK 抗体 (A-10) Alexa Fluor® 488

sc-166765 AF488
200 µg/ml
RMB2685.00

RICK 抗体 (A-10) Alexa Fluor® 546

sc-166765 AF546
200 µg/ml
RMB2685.00

RICK 抗体 (A-10) Alexa Fluor® 594

sc-166765 AF594
200 µg/ml
RMB2685.00

RICK 抗体 (A-10) Alexa Fluor® 647

sc-166765 AF647
200 µg/ml
RMB2685.00

RICK 抗体 (A-10) Alexa Fluor® 680

sc-166765 AF680
200 µg/ml
RMB2685.00

RICK 抗体 (A-10) Alexa Fluor® 790

sc-166765 AF790
200 µg/ml
RMB2685.00

Can RICK (A-10): sc-166765 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, RICK (A-10): sc-166765 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from RICK AbRICK antibody worked well in histology in human tissues
Date published: 2024-07-23
Rated 4 out of 5 by from Nice endogenous Signal in Western Blot from HeLaThe antibody gives a nice specific signal of HeLa endogenous RIP2 in Western Blot. Unfortunately in my hands it did not give a nice cytoplasmic IF staining in PFA-fixed HeLa cells.
Date published: 2018-01-29
Rated 4 out of 5 by from Anti-RICK1 (RIPK2) AntibodyMouse anti-RICK1 antibody (sc-166765) works great on immunoblotting at 1:1000 in 2.5 % milk powder. It works well on IHC at 1:200 and can be reused for immunoblotting. It also works great on IP but not for in vitro kinase assays. It recognizes a 60 kDa band in all cell lines tested so far.
Date published: 2017-08-24
Rated 5 out of 5 by from Brilliant StainingBeautiful cytoplasmic staining seen in IF at a 1:50 dilution.
Date published: 2017-01-17
Rated 4 out of 5 by from Potent and quite specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium detection of the ~61 kDa target protein in a HeLa/Jurkat cell lysate mix, but not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested. A ~46 kDa weak cross-reactive protein was evident in the HeLa/Jurkat cell lysate mix.
Date published: 2016-11-10
Rated 5 out of 5 by from Beautiful immunofluorescence cytoplasmicBeautiful immunofluorescence cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-03
Rated 5 out of 5 by from Produced positive Western Blot data of RICKProduced positive Western Blot data of RICK expression in SK-MEL-28 whole cell lysate. -SCBT QC
Date published: 2014-07-08
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RICK 抗体 (A-10) is rated 4.4 out of 5 by 7.
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