Date published: 2025-9-7
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Ribosomal Protein S14 Antibody (3G5) - Western Blotting - Image 368231
核糖体蛋白 S14 抗体 (3G5): sc-293478. K-562 (A), HeLa (B), Hep G2 (C) 和 RPE-J (D) 全细胞裂解液中核糖体蛋白 S14 表达的 Western 印迹分析.
Ribosomal Protein S14 Antibody (3G5) - Western Blotting - Image 368231
核糖体蛋白 S14 抗体 (3G5): sc-293478. K-562 (A), HeLa (B), Hep G2 (C) 和 RPE-J (D) 全细胞裂解液中核糖体蛋白 S14 表达的 Western 印迹分析.
Ribosomal Protein S14 Antibody (3G5) - Western Blotting - Image 352140
核糖体蛋白 S14 抗体 (3G5): sc-293478. 对 K-562 全细胞裂解液中核糖体蛋白 S14 的表达进行 Western 印迹分析.
Ribosomal Protein S14 Antibody (3G5) - Western Blotting - Image 348830
核糖体蛋白 S14 抗体 (3G5): sc-293478. 人重组核糖体蛋白 S14 融合蛋白的 Western 印迹分析.
核糖体蛋白 S14 抗体 (3G5): sc-293478. K-562 (A), HeLa (B), Hep G2 (C) 和 RPE-J (D) 全细胞裂解液中核糖体蛋白 S14 表达的 Western 印迹分析.

Ribosomal Protein S14 抗体 (3G5): sc-293478

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说明书
  • Ribosomal Protein S14 抗体 3G5 是小鼠单克隆 IgG2a κ，Ribosomal Protein S14抗体, 在3篇文献中引用，规格为100 µg/ml
  • 与氨基酸45-144结合，代表human起源的部分长度Ribosomal Protein S14
  • 推荐用于 mouse, rat 和 human 来源的Ribosomal Protein S14 WB, IP 和 ELISA检测
  • 关于如何获取Ribosomal Protein S14 (3G5): sc-293478的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Ribosomal Protein S14 抗体 (3G5)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

此外 Ribosomal Protein 抗体 包括 Ribosomal Protein L11, Ribosomal Protein L13, Ribosomal Protein L14, Ribosomal Protein L17, Ribosomal Protein L18, Ribosomal Protein L19, Ribosomal Protein L21, Ribosomal Protein L22, Ribosomal Protein L23a和Ribosomal Protein L30

核糖体蛋白S14抗体（3G5）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的核糖体蛋白S14（RPS14）。抗RPS14抗体（3G5）以非结合形式提供。RPS14，也称为EMTB，是小型核糖体40S亚基中一个关键的151个氨基酸的组成部分，在蛋白质合成中起着至关重要的作用。RPS14位于细胞质中，属于S11P家族核糖体蛋白，在各种物种中具有高度保守性，这凸显了其在细胞功能中的根本重要性。核糖体是一种复杂的分子机器，由小亚基（40S）和大亚基（60S）组成，包含80多种不同的核糖体蛋白，对于将信使RNA转化为蛋白质至关重要。RPS14的缺陷会导致对吐根碱（一种强效蛋白质合成抑制剂）产生抗药性，尤其是在中国仓鼠卵巢细胞中。RPS14的多种同源物由可变剪接事件产生，凸显了该蛋白在各种细胞环境中的多功能性和适应性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ribosomal Protein S14 抗体 (3G5) 参考文献:

  1. 编码水稻线粒体 RPS14 和 SDHB 的单个核转录本通过替代剪接加工：不同蛋白质共同使用相同的线粒体靶向信号。  |  Kubo, N., et al. 1999. Proc Natl Acad Sci U S A. 96: 9207-11. PMID: 10430921
  2. 核编码的 SDH2-RPS14 前体在 SDH2 和 RPS14 之间进行蛋白水解处理，生成玉米线粒体 RPS14。  |  Figueroa, P., et al. 2000. Biochem Biophys Res Commun. 271: 380-5. PMID: 10799306
  3. 酵母核糖体蛋白 rpS14 与 RNA 的相互作用。  |  Antúnez de Mayolo, P. and Woolford, JL. 2003. J Mol Biol. 333: 697-709. PMID: 14568531
  4. 酵母核糖体蛋白 S14 的羧基末端延伸是 43S 前核糖体成熟所必需的。  |  Jakovljevic, J., et al. 2004. Mol Cell. 14: 331-42. PMID: 15125836
  5. 原核生物基因组中 S10, spc 和 alpha 核糖体蛋白基因簇的组织。  |  Coenye, T. and Vandamme, P. 2005. FEMS Microbiol Lett. 242: 117-26. PMID: 15621428
  6. 推测的 NTP 酶 Fap7 通过与 Rps14 直接相互作用，介导细胞质 20S pre-RNA 的加工。  |  Granneman, S., et al. 2005. Mol Cell Biol. 25: 10352-64. PMID: 16287850
  7. 通过 RNA 干扰筛选确定 RPS14 为 5q 综合征基因  |  Ebert, BL., et al. 2008. Nature. 451: 335-9. PMID: 18202658
  8. 核糖体应激时，核糖体蛋白 S14 会解开 MDM2-p53 环路。  |  Zhou, X., et al. 2013. Oncogene. 32: 388-96. PMID: 22391559
  9. hCINAP通过控制核糖体蛋白S14的NEDDylation，成为核糖体蛋白-HDM2-p53通路的新型调节因子。  |  Zhang, J., et al. 2014. Oncogene. 33: 246-54. PMID: 23246961
  10. 核糖体蛋白S14沉默通过激活p53抑制由骨髓增生异常综合征转化的急性髓细胞白血病生长。  |  Wang, L., et al. 2014. Hematology. 19: 225-31. PMID: 24074450
  11. 人类 RPS14 等全基因空位的功能分析。  |  Martin-Nieto, J. and Roufa, DJ. 1997. J Cell Sci. 110 (Pt 8): 955-63. PMID: 9152021
  12. 酿酒酵母的核糖体蛋白 S14 通过与 RPS14B 前 mRNA 和 18S rRNA 结合来调节其表达。  |  Fewell, SW. and Woolford, JL. 1999. Mol Cell Biol. 19: 826-34. PMID: 9858605

订购信息

产品名称产品编号规格价格数量收藏夹

Ribosomal Protein S14 抗体 (3G5)

sc-293478
100 µg/ml
$316.00

你们的抗体序列是全长还是部分

Asked by: hailiang
感谢您的提问。sc-293478 Ribosomal Protein S14 (3G5)是针对人源核糖体蛋白S14的部分氨基酸长度作为抗原生产的抗体，抗原氨基酸序列为45-144 Thank you for your question. sc-293478 Ribosomal Protein S14 (3G5) is raised against amino acids 45-144 representing partial length Ribosomal Protein S14 of human origin.
Answered by: Technical Support 8
Date published: 2018-12-21

i work on zebrafish and generally only use polyclonal antibodies for this reason. there is only a single amino acid difference between the human and zebrafish in this region . do you know how likely this is to work? Happy to test an aliquot 

Asked by: Bethpay
If you are working with a cleaved or modified protein or with a protein from a different animal species and if you are concerned about reactivity, please send us your sequence or the protein accession number ([email protected]). We will be more than happy to check the homology and let you know if any of our antibodies will be appropriate for your experiments.
Answered by: Technical Support Europe
Date published: 2017-06-28

What is the recommended starting dilution for Western blot for Ribosomal Protein S14 (3G5): sc-293478 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-293478.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-04-05
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Rated 5 out of 5 by from Good for Western BlotAntibody works for Western blot with extracts from ribosomes isolated from human CS (Cockayne syndrome) fibroblast cells in published research. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from Nice specificity!Detects human and Neurospora crassa RPS14 readily (does not recognize yeast RPS14). Protein easily detected in Western blot (50 µg of total protein, primary Ab diluted 1:200, secondary antibody diluted 1:10,000, detection with Super Signal Pico).
Date published: 2018-02-20
Rated 5 out of 5 by from a fantastic antibody for WBThis antibody recognizes a very clear and strong band of predicted M.W. on the western blot (1:500 dilution, 40 ug U251 lysate).
Date published: 2017-08-28
Rated 5 out of 5 by from Good specificity in WB in target band!In target region of the membrane, this antibody shows a good specificity in WB in PLC whole cell lysate.
Date published: 2017-03-31
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Ribosomal Protein S14抗体 (3G5) is rated 5.0 out of 5 by 4.
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