Date published: 2025-9-7
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Ribosomal Protein L19 Antibody (K-12) - Western Blotting - Image 368280
核糖体蛋白 L19 抗体 (K-12): sc-100830. 核糖体蛋白 L19 在 HeLa (A), K-562 (B), MCF7 (C), RAW 264. 7 (D) 和 NIH/3T3 (E) 全细胞裂解液及大鼠肺组织提取物 (F) 中表达的 Western 印迹分析.
核糖体蛋白 L19 抗体 (K-12): sc-100830. 核糖体蛋白 L19 在 HeLa (A), K-562 (B), MCF7 (C), RAW 264. 7 (D) 和 NIH/3T3 (E) 全细胞裂解液及大鼠肺组织提取物 (F) 中表达的 Western 印迹分析.

Ribosomal Protein L19 抗体 (K-12): sc-100830

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寫評論提問

说明书
  • Ribosomal Protein L19抗体(K-12)是小鼠单克隆IgG2a λ Ribosomal Protein L19抗体, 在19篇文献中引用，规格为100 µg/ml
  • 针对 human 起源的 Ribosomal Protein L19 氨基酸 1-101 内的重组蛋白
  • 推荐用于 mouse, rat 和 human 来源的Ribosomal Protein L19 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取Ribosomal Protein L19 (K-12): sc-100830的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Ribosomal Protein L19 抗体 (K-12)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
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関連項目

此外 Ribosomal Protein 抗体 包括 Ribosomal Protein L11, Ribosomal Protein L13, Ribosomal Protein L14, Ribosomal Protein L17, Ribosomal Protein L18, Ribosomal Protein L19, Ribosomal Protein L21, Ribosomal Protein L22, Ribosomal Protein L23a和Ribosomal Protein L30

核糖体蛋白L19抗体（K-12）是一种IgG2aλ小鼠单克隆核糖体蛋白L19抗体（也称为核糖体蛋白L19抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的核糖体蛋白L19蛋白。核糖体蛋白L19抗体（K-12）可作为非偶联抗核糖体蛋白L19抗体。核糖体是催化蛋白质合成的细胞器，由一个小亚基（40S）和一个由80多种不同核糖体蛋白组成的大亚基（60S）组成。哺乳动物核糖体蛋白由多基因家族编码，这些家族在其编码区中包含加工过的假基因和一个含功能性内含子的基因。核糖体蛋白L19是一种由196个氨基酸组成的蛋白质，是60S亚基的组成部分。核糖体蛋白L19定位于细胞质，属于核糖体蛋白L19e家族，在蛋白质合成中起作用。核糖体蛋白L19的表达在乳腺癌中上调，其过度表达被用作前列腺癌症的预后标志物。与大多数核糖体蛋白一样，核糖体蛋白L19以多个加工过的假基因存在，这些假基因散布在整个基因组中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ribosomal Protein L19 抗体 (K-12) 参考文献:

  1. 乳腺癌细胞系 SKBR3 中差异表达基因的鉴定：新预后生物标志物的潜在鉴定。  |  Leirdal, M., et al. 2004. Int J Mol Med. 14: 217-22. PMID: 15254768
  2. 7 个含内含子的核糖体蛋白基因的图谱显示，它们在人类基因组中是不相连的。  |  Feo, S., et al. 1992. Genomics. 13: 201-7. PMID: 1577483
  3. 大鼠胎儿大脑发育过程中的保守基因表达--通过半定量 RT-PCR 验证。  |  Al-Bader, MD. and Al-Sarraf, HA. 2005. Brain Res Dev Brain Res. 156: 38-45. PMID: 15862626
  4. 核糖体蛋白 l19 是人类前列腺癌的预后标志物。  |  Bee, A., et al. 2006. Clin Cancer Res. 12: 2061-5. PMID: 16609016
  5. 粪便核糖体蛋白 L19 是影响结直肠癌患者生存期的遗传预后因素。  |  Huang, CJ., et al. 2008. J Cell Mol Med. 12: 1936-43. PMID: 18266979
  6. 核糖体蛋白L19被鉴定为肺腺癌中自体细胞毒性T淋巴细胞识别的一种新型肿瘤抗原。  |  Kuroda, K., et al. 2010. Cancer Sci. 101: 46-53. PMID: 19799608
  7. 核糖体蛋白L19的过度表达会激活未折叠蛋白反应，并使MCF7乳腺癌细胞对内质网应激诱导的细胞死亡更敏感。  |  Hong, M., et al. 2014. Biochem Biophys Res Commun. 450: 673-8. PMID: 24950402
  8. 核糖体蛋白 L19 的改变会降低翻译的准确性。  |  VanNice, J., et al. 2016. Biochimie. 128-129: 122-6. PMID: 27477481
  9. 在存在多个加工假基因的情况下检测和分离含内含子基因的策略。  |  Davies, B., et al. 1989. Proc Natl Acad Sci U S A. 86: 6691-5. PMID: 2771953
  10. 核糖体蛋白基因序列映射到人类第 5, 8 和 17 号染色体上。  |  Nakamichi, NN., et al. 1986. Somat Cell Mol Genet. 12: 225-36. PMID: 3459254
  11. 大鼠核糖体蛋白L19的主要结构。根据cDNA中的核苷酸序列和蛋白质中的氨基酸序列确定。  |  Chan, YL., et al. 1987. J Biol Chem. 262: 1111-5. PMID: 3542997
  12. L19 核糖体蛋白基因（RPL19）：基因组织, 染色体图谱和新型启动子区域。  |  Davies, B. and Fried, M. 1995. Genomics. 25: 372-80. PMID: 7789970

订购信息

产品名称产品编号规格价格数量收藏夹

Ribosomal Protein L19 抗体 (K-12)

sc-100830
100 µg/ml
RMB2504.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Ribosomal Protein L19 (K-12): sc-100830 monoclonal antibody?

Asked by: jenniferc
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGλ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Works but relatively weakPurchased for IF application. HEK cell fixed in Methanol (top) and 4% Formaldehyde (bottom) stained with 1:100 @RT for 1hr. Used sc-516190 for 2Ab. It worked in both stain which makes me happy, but signal was pretty weak. (I am using super high gage here...) Also, be aware that this is LAMBDA FRAGMENT. If you are using recent SCBT secondary antibody, most likely it is Kappa which will not bind to lambda. Use either sc-516190, or general anti-mouse (H+L) so that it has affinity to lambda fragment.
Date published: 2019-04-10
Rated 5 out of 5 by from Good signal on WBI tested this antibody in mouse pancreatic cancer line. This produced strong and specific signals (5% milk, 1:200).
Date published: 2018-11-13
Rated 3 out of 5 by from not optimalTested this antibody at the same time as several other monoclonal antibodies against ribosomal proteins. This one had the weakest signal, and the most non-specific bands showing up in Western blots.
Date published: 2018-02-21
Rated 5 out of 5 by from Good publishable data in WB in mouse testesGood publishable data in WB in mouse testes. -SCBT Publication Review
Date published: 2015-05-13
Rated 5 out of 5 by from Strong signal in WB in mouse transfectedStrong signal in WB in mouse transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2015-05-06
Rated 5 out of 5 by from Single band in WB in HeLa whole cell lysateSingle band in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-02-24
Rated 5 out of 5 by from Produced great immunoperoxidase cytoplasmicProduced great immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human small Intestine tissue. -SCBT QC
Date published: 2015-01-24
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Ribosomal Protein L19 抗体 (K-12) is rated 4.6 out of 5 by 7.
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