Date published: 2025-9-7

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Ribophorin II 抗体 (A-1): sc-166421

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说明书
  • Ribophorin II 抗体 A-1 是小鼠单克隆 IgG2a κ,Ribophorin II抗体, 在8篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Ribophorin II的氨基酸332-631
  • Ribophorin II 抗体 (A-1) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Ribophorin II
  • 抗Ribophorin II抗体(A-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ribophorin II (A-1): sc-166421免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是Ribophorin II Antibody (A-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Ribophorin II Antibody (A-1) 打包提供(请参阅下面的订购信息)。

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関連項目

Ribophorin II抗体(A-1)是一种IgG2a κ小鼠单克隆Ribophorin II抗体(也称为RPN2抗体、RPN-II抗体、RIBIIR抗体或Ribophorin-2抗体),它通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的Ribophorin II蛋白。Ribophorin II抗体(A-1)既可作为非偶联的抗Ribophorin II抗体形式提供,也可作为多种偶联的抗Ribophorin II抗体形式提供,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®偶联物。内质网(ER)的膜蛋白可能通过涉及滞留、回收或两者结合的机制进行定位。ER定位信息已在细胞质、跨膜或腔域中发现。已经为含有KDEL结构域的腔ER蛋白和携带双赖氨酸基序的I型跨膜蛋白确定了特定的回收机制。哺乳动物的寡糖基转移酶(OST)是一个由四个粗糙ER特异的I型跨膜蛋白组成的蛋白质复合物:Ribophorins I和II(RI和RII)、OST48和DAD1(也称为抗凋亡死亡防御者)。Ribophorins是完整的膜糖蛋白,专门定位于粗糙ER。DAD1的细胞质定位N端区域与OST48的短细胞质尾部之间存在亲和力,使DAD1牢固地结合到OST复合物中。OST复合物影响新合成多肽的共翻译N-糖基化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ribophorin II 抗体 (A-1) 参考文献:

  1. 低聚糖基转移酶复合物亚基在内质网中的保留。  |  Fu, J. and Kreibich, G. 2000. J Biol Chem. 275: 3984-90. PMID: 10660554
  2. 核糖蛋白 II 在内质网中的定域涉及其跨膜结构域和胞质结构域。  |  Fu, J., et al. 2000. Eur J Cell Biol. 79: 219-28. PMID: 10826490
  3. 哺乳动物寡糖基转移酶复合体的 48 kDa 亚基与酵母必需蛋白 WBP1 同源。  |  Silberstein, S., et al. 1992. J Biol Chem. 267: 23658-63. PMID: 1429707
  4. Ribophorin II促进食管癌细胞在体外和体内的增殖、迁移和侵袭。  |  Li, Y., et al. 2019. Biosci Rep. 39: PMID: 30940778
  5. 核糖核酸酶II在骨髓增生异常综合征中上调,可防止细胞凋亡和细胞周期进展。  |  Ren, J., et al. 2020. Exp Biol Med (Maywood). 245: 1009-1015. PMID: 32447991
  6. 辣椒素通过自噬介导的Ribophorin II下调和坏死性凋亡增强抗癌药物在口腔鳞状细胞癌细胞中的疗效。  |  Huang, YC., et al. 2021. Front Pharmacol. 12: 676813. PMID: 34512323
  7. 在P16阴性的局部晚期头颈部鳞状细胞癌中,Ribophorin II的过度表达与多西他赛、顺铂和氟尿嘧啶诱导化疗的不良反应有关。  |  Chen, WS., et al. 2021. J Clin Med. 10: PMID: 34575229
  8. Ribophorin II通过调节活性氧介导的磷脂酰肌醇-3-激酶/蛋白激酶B的激活,促进喉鳞状细胞癌的上皮-间质转化和有氧糖酵解。  |  Zhou, J., et al. 2022. Bioengineered. 13: 5141-5151. PMID: 35156537
  9. 核糖核酸酶II的下调抑制鼻咽癌的生长、迁移和侵袭[撤回]。  |  . 2023. Onco Targets Ther. 16: 541-542. PMID: 37441363
  10. 防止细胞凋亡的卫士 DAD1 是哺乳动物寡糖基转移酶的一个亚基。  |  Kelleher, DJ. and Gilmore, R. 1997. Proc Natl Acad Sci U S A. 94: 4994-9. PMID: 9144178
  11. 低聚糖基转移酶复合体亚基之间的相互作用。  |  Fu, J., et al. 1997. J Biol Chem. 272: 29687-92. PMID: 9368036
  12. 低聚糖基转移酶复合物的功能和结构完整性需要 DAD1。  |  Sanjay, A., et al. 1998. J Biol Chem. 273: 26094-9. PMID: 9748289

订购信息

产品名称产品编号规格价格数量收藏夹

Ribophorin II 抗体 (A-1)

sc-166421
200 µg/ml
RMB2377.00

Ribophorin II (A-1): m-IgG Fc BP-HRP 套装

sc-528964
200 µg Ab; 10 µg BP
RMB2662.00

Ribophorin II (A-1): m-IgGκ BP-HRP 套装

sc-521603
200 µg Ab, 40 µg BP
RMB2662.00

Ribophorin II (A-1): m-IgG2a BP-HRP 套装

sc-547217
200 µg Ab; 10 µg BP
RMB2662.00

Ribophorin II 抗体 (A-1) AC

sc-166421 AC
500 µg/ml, 25% agarose
RMB3129.00

Ribophorin II 抗体 (A-1) HRP

sc-166421 HRP
200 µg/ml
RMB2377.00

Ribophorin II 抗体 (A-1) FITC

sc-166421 FITC
200 µg/ml
RMB2482.00

Ribophorin II 抗体 (A-1) PE

sc-166421 PE
200 µg/ml
RMB2580.00

Ribophorin II 抗体 (A-1) Alexa Fluor® 488

sc-166421 AF488
200 µg/ml
RMB2685.00

Ribophorin II 抗体 (A-1) Alexa Fluor® 546

sc-166421 AF546
200 µg/ml
RMB2685.00

Ribophorin II 抗体 (A-1) Alexa Fluor® 594

sc-166421 AF594
200 µg/ml
RMB2685.00

Ribophorin II 抗体 (A-1) Alexa Fluor® 647

sc-166421 AF647
200 µg/ml
RMB2685.00

Ribophorin II 抗体 (A-1) Alexa Fluor® 680

sc-166421 AF680
200 µg/ml
RMB2685.00

Ribophorin II 抗体 (A-1) Alexa Fluor® 790

sc-166421 AF790
200 µg/ml
RMB2685.00

What is the recommended fixation method for immunofluorescence staining with Ribophorin II (A-1): sc-166421 monoclonal antibody?

Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent Antibody! Highly recommend :)I got the free sample of RPN2 antibody, and I checked on different prostate cancer cell lines. Well, The results were amazing! I got a strong and specific band, I am really happy with this Ab, and today I am going to order this Ab for further experiments :) Thank Santa Cruz very much!!!
Date published: 2018-02-21
Rated 5 out of 5 by from 效果非常好第一次跑就跑出来了,效果非常好,会继续购买正品的,感谢贵公司的试用装,对我们实验室帮助非常大,非常感谢
Date published: 2017-12-18
Rated 5 out of 5 by from Excellent antibody in WB at 1:1000.Excellent antibody in WB at 1:1000. Antibody with high specificity can be used for further experiments.
Date published: 2017-11-23
Rated 5 out of 5 by from Nice detection of Ribophorin2 in HeLa cellsWe used this antibody for expression detection of Ribophorin2 by western blot. We got a very nice signal around the correct molecular weight in HeLa cells.
Date published: 2017-02-09
Rated 5 out of 5 by from IP and WB publishable data with human 293T cellsIP and WB publishable data with human 293T cells - SCBT Publication Review
Date published: 2015-04-11
Rated 5 out of 5 by from Single strong band at correct mw in NIH/3T3Single strong band at correct mw in NIH/3T3 whole cell lysate. -SCBT QC
Date published: 2015-03-09
Rated 5 out of 5 by from Superb immunofluorescence cytoplasmic stainingSuperb immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2013-12-09
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Ribophorin II 抗体 (A-1) is rated 5.0 out of 5 by 7.
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