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Rhotekin 抗体 (B-4): sc-393833

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说明书
  • Rhotekin 抗体 B-4 是小鼠单克隆 IgG2a (kappa轻链),Rhotekin抗体,规格为200 µg/ml
  • 免疫原氨基酸序列216-467位于human物种的Rhotekin的内部区域内
  • Rhotekin 抗体 (B-4) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Rhotekin
  • 抗Rhotekin抗体(B-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rhotekin (B-4): sc-393833免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是Rhotekin Antibody (B-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Rhotekin Antibody (B-4) 打包提供(请参阅下面的订购信息)。

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    関連項目

    Rhotekin抗体(B-4)是一种IgG2a κ小鼠单克隆Rhotekin抗体(也称为Rhotekin抗体),可通过WB、IP、IF和ELISA等方法检测小鼠、大鼠和人类来源的Rhotekin蛋白。Rhotekin抗体(B-4)既可作为非偶联的抗Rhotekin抗体形式提供,也可作为多种抗Rhotekin抗体的偶联形式提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Ras相关的小GTP酶Rho负责调节基于肌动蛋白的细胞骨架结构,包括应力纤维、粘着斑和收缩环装置。Rho蛋白作为分子开关,能够开启和关闭细胞质分裂。尽管人们对Rho下游的信号传导知之甚少,但已经描述了许多假定的Rho效应蛋白,包括rhophilin、Rhotekin、citron以及丝氨酸/苏氨酸激酶、蛋白激酶N。另外还描述了两个Rho激活的丝氨酸/苏氨酸激酶,分别命名为Rock-1和Rock-2(也称为Roka),即Rho相关含卷曲蛋白激酶。Rock-1和Rock-2与强直性肌营养不良激酶具有结构相似性。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Rhotekin 抗体 (B-4) 参考文献:

    1. 一种新型丝氨酸/苏氨酸激酶与 Ras 相关的 RhoA GTPase 结合,从而将激酶转运到外周膜。  |  Leung, T., et al. 1995. J Biol Chem. 270: 29051-4. PMID: 7493923
    2. 大鼠睾丸脂肪酸活化蛋白激酶(PKN)的纯化和表征。  |  Kitagawa, M., et al. 1995. Biochem J. 310 (Pt 2): 657-64. PMID: 7654208
    3. 蛋白激酶 N(PKN)和 PKN 相关蛋白 rhophilin 是小 GTPase Rho 的靶标。  |  Watanabe, G., et al. 1996. Science. 271: 645-8. PMID: 8571126
    4. 确定丝氨酸-苏氨酸激酶蛋白激酶 N 是 Rho 的假定靶标。  |  Amano, M., et al. 1996. Science. 271: 648-50. PMID: 8571127
    5. PKN氨基末端,N端独特基团对其酶活性的作用。  |  Kitagawa, M., et al. 1996. Biochem Biophys Res Commun. 220: 963-8. PMID: 8607876
    6. 小 GTP 结合蛋白 Rho 与一个与肌营养不良症激酶同源的 160 kDa Ser/Thr 蛋白激酶结合并激活该激酶。  |  Ishizaki, T., et al. 1996. EMBO J. 15: 1885-93. PMID: 8617235
    7. PKN 与神经丝蛋白的头棒结构域结合并使其磷酸化。  |  Mukai, H., et al. 1996. J Biol Chem. 271: 9816-22. PMID: 8621664
    8. RhoA 与 PKN 氨基末端,N 端之间相互作用的特征。  |  Shibata, H., et al. 1996. FEBS Lett. 385: 221-4. PMID: 8647255

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Rhotekin 抗体 (B-4)

    sc-393833
    200 µg/ml
    RMB2377.00

    Rhotekin (B-4): m-IgG Fc BP-HRP 套装

    sc-530596
    200 µg Ab; 10 µg BP
    RMB2662.00

    Rhotekin (B-4): m-IgGκ BP-HRP 套装

    sc-524110
    200 µg Ab, 40 µg BP
    RMB2662.00

    Rhotekin (B-4): m-IgG2a BP-HRP 套装

    sc-547528
    200 µg Ab; 10 µg BP
    RMB2662.00

    Rhotekin 抗体 (B-4) AC

    sc-393833 AC
    500 µg/ml, 25% agarose
    RMB3129.00

    Rhotekin 抗体 (B-4) HRP

    sc-393833 HRP
    200 µg/ml
    RMB2377.00

    Rhotekin 抗体 (B-4) FITC

    sc-393833 FITC
    200 µg/ml
    RMB2482.00

    Rhotekin 抗体 (B-4) PE

    sc-393833 PE
    200 µg/ml
    RMB2580.00

    Rhotekin 抗体 (B-4) Alexa Fluor® 488

    sc-393833 AF488
    200 µg/ml
    RMB2685.00

    Rhotekin 抗体 (B-4) Alexa Fluor® 546

    sc-393833 AF546
    200 µg/ml
    RMB2685.00

    Rhotekin 抗体 (B-4) Alexa Fluor® 594

    sc-393833 AF594
    200 µg/ml
    RMB2685.00

    Rhotekin 抗体 (B-4) Alexa Fluor® 647

    sc-393833 AF647
    200 µg/ml
    RMB2685.00

    Rhotekin 抗体 (B-4) Alexa Fluor® 680

    sc-393833 AF680
    200 µg/ml
    RMB2685.00

    Rhotekin 抗体 (B-4) Alexa Fluor® 790

    sc-393833 AF790
    200 µg/ml
    RMB2685.00

    I need to use an antibody to detect the protein in samples of canine origin. Do you know if Rhotekin (B-4): sc-393833 mouse monoclonal antibody will work?

    Asked by: Trav11
    Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
    Answered by: Technical Support
    Date published: 2017-03-01
    • y_2025, m_9, d_7, h_4CST
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    Rated 5 out of 5 by from Good staining.The antibody was tested on mouse sections and it works well, with a clear enough signal. IF dilution 1:100.
    Date published: 2022-07-12
    Rated 5 out of 5 by from No signal w/ primary BMMs, works w/ HEK and NIH3T3I tested free sample for Western Blot (1 ug/ml Ab, o/n incubation, Li-Cor detection) with mouse primary macrophages and did not see any signal around 60 kDa. Antibody reacts with HEK cells and NIH 3T3 that I run on the same gel. It looks like RTKN expression level is low in BMMs. Under stimulation conditions there were no signal as well. Thanks for the free sample program!
    Date published: 2018-02-06
    Rated 5 out of 5 by from Satisfactory Western blot data of RhotekinSatisfactory Western blot data of Rhotekin expression in non-transfected 293T, human Rhotekin transfected 293T A-431 and Jurkat whole cell lysates. -SCBT QC
    Date published: 2013-07-16
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    Rhotekin 抗体 (B-4) is rated 5.0 out of 5 by 3.
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